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FRET-mediated long-range wavelength transformation by photoconvertible fluorescent proteins as an efficient mechanism to generate orange-red light in symbiotic deep water corals

FRET-mediated long-range wavelength transformation by photoconvertible fluorescent proteins as an efficient mechanism to generate orange-red light in symbiotic deep water corals
FRET-mediated long-range wavelength transformation by photoconvertible fluorescent proteins as an efficient mechanism to generate orange-red light in symbiotic deep water corals
Photoconvertible fluorescent proteins (pcRFPs) are a group of fluorophores that undergo an irreversible green-to-red shift in emission colour upon irradiation with near-ultraviolet (near-UV) light. Despite their wide application in biotechnology, the high-level expression of pcRFPs in mesophotic and depth-generalist coral species currently lacks a biological explanation. Additionally, reduced penetration of near-UV wavelengths in water poses the question whether light-driven photoconversion is relevant in the mesophotic zone, or whether a different mechanism is involved in the post-translational pigment modification in vivo. Here, we show in a long-term mesocosm experiment that photoconversion in vivo is entirely dependent on near-UV wavelengths. However, a near-UV intensity equivalent to the mesophotic underwater light field at 80 m depth is sufficient to drive the process in vitro, suggesting that photoconversion can occur near the lower distribution limits of these corals. Furthermore, live coral colonies showed evidence of efficient Förster Resonance Energy Transfer (FRET). Our simulated mesophotic light field maintained the pcRFP pool in a partially photoconverted state in vivo, maximising intra-tetrameric FRET and creating a long-range wavelength conversion system with higher quantum yield than other native RFPs. We hypothesise that efficient conversion of blue wavelengths, abundant at depth, into orange-red light could constitute an adaptation of corals to life in light-limited environments. View Full-Text
1422-0067
Bollati, Elena
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Plimmer, Daniel
490d1ab5-ed69-4756-9727-deab5ca064c7
D'Angelo, Cecilia
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Wiedenmann, Jörg
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Bollati, Elena
5d9ec6e5-83e3-41b3-91ce-aa112949cd1f
Plimmer, Daniel
490d1ab5-ed69-4756-9727-deab5ca064c7
D'Angelo, Cecilia
0d35b03b-684d-43aa-a57a-87212ab07ee1
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7

Bollati, Elena, Plimmer, Daniel, D'Angelo, Cecilia and Wiedenmann, Jörg (2017) FRET-mediated long-range wavelength transformation by photoconvertible fluorescent proteins as an efficient mechanism to generate orange-red light in symbiotic deep water corals. International Journal of Molecular Sciences, 18 (7), [1174]. (doi:10.3390/ijms18071174).

Record type: Article

Abstract

Photoconvertible fluorescent proteins (pcRFPs) are a group of fluorophores that undergo an irreversible green-to-red shift in emission colour upon irradiation with near-ultraviolet (near-UV) light. Despite their wide application in biotechnology, the high-level expression of pcRFPs in mesophotic and depth-generalist coral species currently lacks a biological explanation. Additionally, reduced penetration of near-UV wavelengths in water poses the question whether light-driven photoconversion is relevant in the mesophotic zone, or whether a different mechanism is involved in the post-translational pigment modification in vivo. Here, we show in a long-term mesocosm experiment that photoconversion in vivo is entirely dependent on near-UV wavelengths. However, a near-UV intensity equivalent to the mesophotic underwater light field at 80 m depth is sufficient to drive the process in vitro, suggesting that photoconversion can occur near the lower distribution limits of these corals. Furthermore, live coral colonies showed evidence of efficient Förster Resonance Energy Transfer (FRET). Our simulated mesophotic light field maintained the pcRFP pool in a partially photoconverted state in vivo, maximising intra-tetrameric FRET and creating a long-range wavelength conversion system with higher quantum yield than other native RFPs. We hypothesise that efficient conversion of blue wavelengths, abundant at depth, into orange-red light could constitute an adaptation of corals to life in light-limited environments. View Full-Text

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ijms-18-01174 - Version of Record
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Accepted/In Press date: 17 May 2017
e-pub ahead of print date: 4 July 2017

Identifiers

Local EPrints ID: 413325
URI: http://eprints.soton.ac.uk/id/eprint/413325
ISSN: 1422-0067
PURE UUID: d3279e77-5edb-449e-8086-d2fb214b67cf
ORCID for Elena Bollati: ORCID iD orcid.org/0000-0003-3536-4587
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

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Date deposited: 22 Aug 2017 16:31
Last modified: 10 Dec 2019 01:42

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