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Extracellular vesicle flow cytometry analysis and standardization

Extracellular vesicle flow cytometry analysis and standardization
Extracellular vesicle flow cytometry analysis and standardization
The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics.
extracellular vesicles, Flow Cytometry, scattering, Fluorescence, standardisation, scattering standardisation
Welsh, Joshua A.
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Wilkinson, James S.
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Holloway, Judith A.
f22f45f3-6fc8-4a4c-bc6c-24add507037c
Englyst, Nicola A.
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Welsh, Joshua A.
fd455949-5aab-442f-83f4-74ff62f41635
Wilkinson, James S.
73483cf3-d9f2-4688-9b09-1c84257884ca
Holloway, Judith A.
f22f45f3-6fc8-4a4c-bc6c-24add507037c
Englyst, Nicola A.
f84399af-7265-4224-b556-102c3aa272b0

Welsh, Joshua A., Wilkinson, James S., Holloway, Judith A. and Englyst, Nicola A. (2017) Extracellular vesicle flow cytometry analysis and standardization. Frontiers in Cell and Developmental Biology, 5. (doi:10.3389/fcell.2017.00078).

Record type: Review

Abstract

The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics.

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Accepted/In Press date: 16 August 2017
e-pub ahead of print date: 30 August 2017
Published date: August 2017
Keywords: extracellular vesicles, Flow Cytometry, scattering, Fluorescence, standardisation, scattering standardisation

Identifiers

Local EPrints ID: 413432
URI: https://eprints.soton.ac.uk/id/eprint/413432
PURE UUID: dd0055df-259c-45b2-8bc0-140c7e53ecae
ORCID for James S. Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697
ORCID for Judith A. Holloway: ORCID iD orcid.org/0000-0002-2268-3071

Catalogue record

Date deposited: 24 Aug 2017 16:30
Last modified: 10 Dec 2019 01:59

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