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DNA methylation profiles between airway epithelium and proxy tissues in children

DNA methylation profiles between airway epithelium and proxy tissues in children
DNA methylation profiles between airway epithelium and proxy tissues in children

Aim:

Epidemiological studies of deoxyribonucleic acid (DNA) methylation in airway disease have largely been conducted using blood or buccal samples. However, given tissue specificity of DNA methylation, these surrogate tissues may not allow reliable inferences about methylation in the lung. We sought to compare the pattern of DNA methylation in blood, buccal and nasal epithelial cells to that in airway epithelial cells from children.


Methods:

Samples of blood, and buccal, nasal and airway epithelium were obtained from six children undergoing elective anaesthesia for adenotonsillectomy. DNA methylation was assessed at 450 000 5′-C—phosphate—G-3′ (CpG) sites using the Illumina HumanMethylation450 array.


Results:

Eighteen samples from all sites were suitable for analysis. Hierarchical clustering demonstrated that the methylation profile in nasal epithelium was most representative of that in airway epithelium; the profile in buccal cells was moderately similar and that in blood was least similar.


Conclusion:

DNA methylation in blood poorly reflects methylation in airway epithelium. Future epidemiological studies of DNA methylation and airway diseases should consider measurement of methylation either in buccal cells or, preferably, in nasal epithelial cells.
0803-5253
2011-2016
Brugha, Rossa
6637a95d-0b09-4e3b-838b-b62329462844
Lowe, Robert
0939e340-8ae4-4f27-a560-33545b392755
Henderson, A. John
2053b8a0-1b2d-41f6-8b3f-1cf360c88c21
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Rakyan, Vardhman
dde7e10b-0c19-4d55-83e0-beeed948f82c
Wozniak, Eva
6755b2d1-4542-4b3f-add1-d8e0197014e8
Mahmud, Nadiya
20065183-dcc8-4628-962e-eb33c7663d04
Seymour, Kay
04dac188-30c0-48cd-bd97-417338715b1f
Grigg, Jonathan
587e2313-7a36-4d61-8010-45447565cfcd
Shaheen, Seif O.
42e3b3fc-c70c-49e7-8c88-64f2606129ec
Brugha, Rossa
6637a95d-0b09-4e3b-838b-b62329462844
Lowe, Robert
0939e340-8ae4-4f27-a560-33545b392755
Henderson, A. John
2053b8a0-1b2d-41f6-8b3f-1cf360c88c21
Holloway, John
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Rakyan, Vardhman
dde7e10b-0c19-4d55-83e0-beeed948f82c
Wozniak, Eva
6755b2d1-4542-4b3f-add1-d8e0197014e8
Mahmud, Nadiya
20065183-dcc8-4628-962e-eb33c7663d04
Seymour, Kay
04dac188-30c0-48cd-bd97-417338715b1f
Grigg, Jonathan
587e2313-7a36-4d61-8010-45447565cfcd
Shaheen, Seif O.
42e3b3fc-c70c-49e7-8c88-64f2606129ec

Brugha, Rossa, Lowe, Robert, Henderson, A. John, Holloway, John, Rakyan, Vardhman, Wozniak, Eva, Mahmud, Nadiya, Seymour, Kay, Grigg, Jonathan and Shaheen, Seif O. (2017) DNA methylation profiles between airway epithelium and proxy tissues in children. Acta Paediatrica, 106 (12), 2011-2016. (doi:10.1111/apa.14027).

Record type: Article

Abstract


Aim:

Epidemiological studies of deoxyribonucleic acid (DNA) methylation in airway disease have largely been conducted using blood or buccal samples. However, given tissue specificity of DNA methylation, these surrogate tissues may not allow reliable inferences about methylation in the lung. We sought to compare the pattern of DNA methylation in blood, buccal and nasal epithelial cells to that in airway epithelial cells from children.


Methods:

Samples of blood, and buccal, nasal and airway epithelium were obtained from six children undergoing elective anaesthesia for adenotonsillectomy. DNA methylation was assessed at 450 000 5′-C—phosphate—G-3′ (CpG) sites using the Illumina HumanMethylation450 array.


Results:

Eighteen samples from all sites were suitable for analysis. Hierarchical clustering demonstrated that the methylation profile in nasal epithelium was most representative of that in airway epithelium; the profile in buccal cells was moderately similar and that in blood was least similar.


Conclusion:

DNA methylation in blood poorly reflects methylation in airway epithelium. Future epidemiological studies of DNA methylation and airway diseases should consider measurement of methylation either in buccal cells or, preferably, in nasal epithelial cells.

Text
Airway DNA methylation Acta Paed 08052017 submitted - Accepted Manuscript
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More information

Accepted/In Press date: 15 August 2017
e-pub ahead of print date: 18 August 2017
Published date: December 2017

Identifiers

Local EPrints ID: 413443
URI: https://eprints.soton.ac.uk/id/eprint/413443
ISSN: 0803-5253
PURE UUID: 21a4c31c-4ace-43ed-8cab-31dcd97b6bcf
ORCID for John Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 24 Aug 2017 16:30
Last modified: 14 Mar 2019 05:38

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Contributors

Author: Rossa Brugha
Author: Robert Lowe
Author: A. John Henderson
Author: John Holloway ORCID iD
Author: Vardhman Rakyan
Author: Eva Wozniak
Author: Nadiya Mahmud
Author: Kay Seymour
Author: Jonathan Grigg
Author: Seif O. Shaheen

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