Stable isotope analysis of dynamic lipidomics
Stable isotope analysis of dynamic lipidomics
Metabolic pathway flux is a fundamental element of biological activity, which can be quantified using a variety of mass spectrometric techniques to monitor incorporation of stable isotope-labelled substrates into metabolic products. This article contrasts developments in electrospray ionisation mass spectrometry (ESI-MS) for the measurement of lipid metabolism with more established gas chromatography mass spectrometry and isotope ratio mass spectrometry methodologies. ESI-MS combined with diagnostic tandem MS/MS scans permits the sensitive and specific analysis of stable isotope-labelled substrates into intact lipid molecular species without the requirement for lipid hydrolysis and derivatisation. Such dynamic lipidomic methodologies using non-toxic stable isotopes can be readily applied to quantify lipid metabolic fluxes in clinical and metabolic studies in vivo. However, a significant current limitation is the absence of appropriate software to generate kinetic models of substrate incorporation into multiple products in the time domain. Finally, we discuss the future potential of stable isotope-mass spectrometry imaging to quantify the location as well as the extent of lipid synthesis.
792-796
Brandsma, Joost
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Bailey, Andrew P.
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Koster, Grielof
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Gould, Alex P.
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Postle, Anthony D.
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August 2017
Brandsma, Joost
b4c553dc-9444-466a-b352-25fd8fa25ee7
Bailey, Andrew P.
d84fd55c-b48f-449b-818a-b044e519bd79
Koster, Grielof
e404c38a-6f48-430a-adf0-5208228cb9e7
Gould, Alex P.
8d8c5c3e-b0b7-4e45-913b-e28d68081844
Postle, Anthony D.
0fa17988-b4a0-4cdc-819a-9ae15c5dad66
Brandsma, Joost, Bailey, Andrew P., Koster, Grielof, Gould, Alex P. and Postle, Anthony D.
(2017)
Stable isotope analysis of dynamic lipidomics.
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1862 (8), .
(doi:10.1016/j.bbalip.2017.03.002).
Abstract
Metabolic pathway flux is a fundamental element of biological activity, which can be quantified using a variety of mass spectrometric techniques to monitor incorporation of stable isotope-labelled substrates into metabolic products. This article contrasts developments in electrospray ionisation mass spectrometry (ESI-MS) for the measurement of lipid metabolism with more established gas chromatography mass spectrometry and isotope ratio mass spectrometry methodologies. ESI-MS combined with diagnostic tandem MS/MS scans permits the sensitive and specific analysis of stable isotope-labelled substrates into intact lipid molecular species without the requirement for lipid hydrolysis and derivatisation. Such dynamic lipidomic methodologies using non-toxic stable isotopes can be readily applied to quantify lipid metabolic fluxes in clinical and metabolic studies in vivo. However, a significant current limitation is the absence of appropriate software to generate kinetic models of substrate incorporation into multiple products in the time domain. Finally, we discuss the future potential of stable isotope-mass spectrometry imaging to quantify the location as well as the extent of lipid synthesis.
Text
BBA Opinion dynamic lipidomics 2017
- Accepted Manuscript
More information
Accepted/In Press date: 9 March 2017
e-pub ahead of print date: 14 March 2017
Published date: August 2017
Identifiers
Local EPrints ID: 413546
URI: http://eprints.soton.ac.uk/id/eprint/413546
ISSN: 1388-1981
PURE UUID: f357b2c3-519d-4616-9afb-1a97a72c06f5
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Date deposited: 25 Aug 2017 16:31
Last modified: 16 Mar 2024 02:32
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Contributors
Author:
Joost Brandsma
Author:
Andrew P. Bailey
Author:
Grielof Koster
Author:
Alex P. Gould
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