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Bioelectrospray methodology for dissection of the host-pathogen interaction in human tuberculosis

Bioelectrospray methodology for dissection of the host-pathogen interaction in human tuberculosis
Bioelectrospray methodology for dissection of the host-pathogen interaction in human tuberculosis
Standard cell culture models have been used to investigate disease pathology and to test new therapies for over fifty years. However, these model systems have often failed to mimic the changes occurring within three-dimensional (3-D) space where pathology occurs in vivo. To truthfully represent this, an emerging paradigm in biology is the importance of modelling disease in a physiologically relevant 3-D environment. One of the approaches for 3-D cell culture is bioelectrospray technology. This technique uses an alginate-based 3-D environment as an inert backbone within which mammalian cells and extracellular matrix can be incorporated. These alginate-based matrices produce highly reproducible results and can be mixed with different extracellular matrix components. This protocol describes a 3-D system incorporating mycobacteria, primary human blood mononuclear cells and collagen-alginate matrix to dissect the host-pathogen interaction in tuberculosis.
1-14
Tezera, Liku B.
c5598dbf-23a8-4934-96a4-7c783bf9e776
Bielecka, Magdalena K.
90391ea3-aa1f-4104-a893-568c138718a2
Elkington, Paul T.
60828c7c-3d32-47c9-9fcc-6c4c54c35a15
Tezera, Liku B.
c5598dbf-23a8-4934-96a4-7c783bf9e776
Bielecka, Magdalena K.
90391ea3-aa1f-4104-a893-568c138718a2
Elkington, Paul T.
60828c7c-3d32-47c9-9fcc-6c4c54c35a15

Tezera, Liku B., Bielecka, Magdalena K. and Elkington, Paul T. (2017) Bioelectrospray methodology for dissection of the host-pathogen interaction in human tuberculosis. Bio-protocol, 7 (14), 1-14. (doi:10.21769/BioProtoc.2418).

Record type: Article

Abstract

Standard cell culture models have been used to investigate disease pathology and to test new therapies for over fifty years. However, these model systems have often failed to mimic the changes occurring within three-dimensional (3-D) space where pathology occurs in vivo. To truthfully represent this, an emerging paradigm in biology is the importance of modelling disease in a physiologically relevant 3-D environment. One of the approaches for 3-D cell culture is bioelectrospray technology. This technique uses an alginate-based 3-D environment as an inert backbone within which mammalian cells and extracellular matrix can be incorporated. These alginate-based matrices produce highly reproducible results and can be mixed with different extracellular matrix components. This protocol describes a 3-D system incorporating mycobacteria, primary human blood mononuclear cells and collagen-alginate matrix to dissect the host-pathogen interaction in tuberculosis.

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Bio-protocol2418 - Version of Record
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Published date: 20 July 2017

Identifiers

Local EPrints ID: 413568
URI: http://eprints.soton.ac.uk/id/eprint/413568
PURE UUID: a0587acc-f454-45b7-9e73-2747324ec657
ORCID for Liku B. Tezera: ORCID iD orcid.org/0000-0002-7898-6709
ORCID for Paul T. Elkington: ORCID iD orcid.org/0000-0003-0390-0613

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Date deposited: 29 Aug 2017 16:30
Last modified: 16 Mar 2024 04:13

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Contributors

Author: Liku B. Tezera ORCID iD
Author: Magdalena K. Bielecka

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