The University of Southampton
University of Southampton Institutional Repository

Bioelectrospray methodology for dissection of the host-pathogen interaction in human tuberculosis

Bioelectrospray methodology for dissection of the host-pathogen interaction in human tuberculosis
Bioelectrospray methodology for dissection of the host-pathogen interaction in human tuberculosis
Standard cell culture models have been used to investigate disease pathology and to test new therapies for over fifty years. However, these model systems have often failed to mimic the changes occurring within three-dimensional (3-D) space where pathology occurs in vivo. To truthfully represent this, an emerging paradigm in biology is the importance of modelling disease in a physiologically relevant 3-D environment. One of the approaches for 3-D cell culture is bioelectrospray technology. This technique uses an alginate-based 3-D environment as an inert backbone within which mammalian cells and extracellular matrix can be incorporated. These alginate-based matrices produce highly reproducible results and can be mixed with different extracellular matrix components. This protocol describes a 3-D system incorporating mycobacteria, primary human blood mononuclear cells and collagen-alginate matrix to dissect the host-pathogen interaction in tuberculosis.
1-14
Tezera, Liku B.
c5598dbf-23a8-4934-96a4-7c783bf9e776
Bielecka, Magdalena K.
90391ea3-aa1f-4104-a893-568c138718a2
Elkington, Paul T.
60828c7c-3d32-47c9-9fcc-6c4c54c35a15
Tezera, Liku B.
c5598dbf-23a8-4934-96a4-7c783bf9e776
Bielecka, Magdalena K.
90391ea3-aa1f-4104-a893-568c138718a2
Elkington, Paul T.
60828c7c-3d32-47c9-9fcc-6c4c54c35a15

Tezera, Liku B., Bielecka, Magdalena K. and Elkington, Paul T. (2017) Bioelectrospray methodology for dissection of the host-pathogen interaction in human tuberculosis. Bio-protocol, 7 (14), 1-14. (doi:10.21769/BioProtoc.2418).

Record type: Article

Abstract

Standard cell culture models have been used to investigate disease pathology and to test new therapies for over fifty years. However, these model systems have often failed to mimic the changes occurring within three-dimensional (3-D) space where pathology occurs in vivo. To truthfully represent this, an emerging paradigm in biology is the importance of modelling disease in a physiologically relevant 3-D environment. One of the approaches for 3-D cell culture is bioelectrospray technology. This technique uses an alginate-based 3-D environment as an inert backbone within which mammalian cells and extracellular matrix can be incorporated. These alginate-based matrices produce highly reproducible results and can be mixed with different extracellular matrix components. This protocol describes a 3-D system incorporating mycobacteria, primary human blood mononuclear cells and collagen-alginate matrix to dissect the host-pathogen interaction in tuberculosis.

Text
Bio-protocol2418 - Version of Record
Available under License Creative Commons Attribution.
Download (5MB)

More information

Published date: 20 July 2017

Identifiers

Local EPrints ID: 413568
URI: https://eprints.soton.ac.uk/id/eprint/413568
PURE UUID: a0587acc-f454-45b7-9e73-2747324ec657
ORCID for Liku B. Tezera: ORCID iD orcid.org/0000-0002-7898-6709
ORCID for Paul T. Elkington: ORCID iD orcid.org/0000-0003-0390-0613

Catalogue record

Date deposited: 29 Aug 2017 16:30
Last modified: 14 Mar 2019 01:35

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×