Tenascin-X increases the stiffness of collagen gels without affecting fibrillogenesis
Tenascin-X increases the stiffness of collagen gels without affecting fibrillogenesis
Tenascin-X is an extracellular matrix protein whose absence leads to an Ehlers-Danlos Syndrome in humans, mainly characterised by connective tissue defects including the disorganisation of fibrillar networks, a reduced collagen deposition, and modifications in the mechanical properties of dense tissues. Here we tested the effect of tenascin-X on in vitro collagen fibril formation. We observed that the main parameters of fibrillogenesis were unchanged, and that the diameter of fibrils was not significantly different when they were formed in the presence of tenascin-X. Interestingly, mechanical analysis of collagen gels showed an increased compressive resistance of the gels containing tenascin-X, indicating that this protein might be directly involved in determining the mechanical properties of collagen-rich tissues in vivo.
Animals, Biophysical Phenomena, Cattle, Collagen, Humans, Microscopy, Electron, Scanning, Rats, Tenascin, Journal Article, Research Support, Non-U.S. Gov't
87-91
Margaron, Yoran
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Bostan, Luciana
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Exposito, Jean-Yves
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Malbouyres, Maryline
b4a1e45d-bf72-422a-8653-a21ae5c10be5
Trunfio-Sfarghiu, Ana-Maria
355c5126-1ac4-42b0-bef1-3f4ffccd7d23
Berthier, Yves
3b244fc9-1770-4415-b5bb-e8e087c5588a
Lethias, Claire
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March 2010
Margaron, Yoran
dda6ac31-53ce-4280-a3f2-f5d0f60382f2
Bostan, Luciana
9b269056-e210-4ab7-815a-f373528dcd66
Exposito, Jean-Yves
f0f4bc4a-8e75-44dd-b616-c30b07353e05
Malbouyres, Maryline
b4a1e45d-bf72-422a-8653-a21ae5c10be5
Trunfio-Sfarghiu, Ana-Maria
355c5126-1ac4-42b0-bef1-3f4ffccd7d23
Berthier, Yves
3b244fc9-1770-4415-b5bb-e8e087c5588a
Lethias, Claire
b2866bb5-69a3-481c-960c-67300d7c3ee4
Margaron, Yoran, Bostan, Luciana, Exposito, Jean-Yves, Malbouyres, Maryline, Trunfio-Sfarghiu, Ana-Maria, Berthier, Yves and Lethias, Claire
(2010)
Tenascin-X increases the stiffness of collagen gels without affecting fibrillogenesis.
Biophysical Chemistry, 147 (1-2), .
(doi:10.1016/j.bpc.2009.12.011).
Abstract
Tenascin-X is an extracellular matrix protein whose absence leads to an Ehlers-Danlos Syndrome in humans, mainly characterised by connective tissue defects including the disorganisation of fibrillar networks, a reduced collagen deposition, and modifications in the mechanical properties of dense tissues. Here we tested the effect of tenascin-X on in vitro collagen fibril formation. We observed that the main parameters of fibrillogenesis were unchanged, and that the diameter of fibrils was not significantly different when they were formed in the presence of tenascin-X. Interestingly, mechanical analysis of collagen gels showed an increased compressive resistance of the gels containing tenascin-X, indicating that this protein might be directly involved in determining the mechanical properties of collagen-rich tissues in vivo.
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Accepted/In Press date: 27 December 2009
e-pub ahead of print date: 4 January 2010
Published date: March 2010
Keywords:
Animals, Biophysical Phenomena, Cattle, Collagen, Humans, Microscopy, Electron, Scanning, Rats, Tenascin, Journal Article, Research Support, Non-U.S. Gov't
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Local EPrints ID: 413668
URI: http://eprints.soton.ac.uk/id/eprint/413668
ISSN: 0301-4622
PURE UUID: d5f458b9-3723-41b0-98a7-aecf23066eef
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Date deposited: 31 Aug 2017 16:31
Last modified: 15 Mar 2024 15:51
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Author:
Yoran Margaron
Author:
Luciana Bostan
Author:
Jean-Yves Exposito
Author:
Maryline Malbouyres
Author:
Ana-Maria Trunfio-Sfarghiu
Author:
Yves Berthier
Author:
Claire Lethias
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