Increased N affects P uptake of eight grassland species: The role of root surface phosphatase activity

Fujita, Yuki, Robroek, Bjorn J M, de Ruiter, Peter C., Heil, Gerrit W. and Wassen, Martin J. (2010) Increased N affects P uptake of eight grassland species: The role of root surface phosphatase activity. Oikos, 119 (10), 1665-1673. (doi:10.1111/j.1600-0706.2010.18427.x).

Abstract

Increased N deposition may change species composition in grassland communities by shifting them to P limitation. Interspecific differences in P uptake traits might be a crucial yet poorly understood factor in determining the N effects. To test the effects of increased N supply (relative to P), we conducted two greenhouse fertilization experiments with eight species from two functional groups (grasses, herbs), including those common in P and N limited grasslands. We investigated plant growth and P uptake from two P sources, orthophosphate and not-readily available P (bound-P), under different N supply levels. Furthermore, to test if the N effects on P uptake was due to N availability alone or altered N:P ratio, we examined several uptake traits (root-surface phosphatase activity, specific root length (SRL), root mass ratio (RMR)) under varying N:P supply ratios. Only a few species (M. caerulea, A. capillaris, S. pratensis) could take up a similar amount of P from bound-P to that from orthophosphate. These species had neither higher SRL, RMR, phosphatase activity per unit root (Pase_root), nor higher total phosphatase activity (Pase_tot: Pase_root times root mass), but higher relative phosphatase activity (Pase_rel: Pase_tot divided by biomass) than other species. The species common from P-limited grasslands had high Pase_rel. P uptake from bound-P was positively correlated with Pase_tot for grasses. High N supply stimulated phosphatase activity but decreased RMR and SRL, resulting in no increase in P uptake from bound-P. Pase_root was influenced by N:P supply ratio, rather than by only N or P level, whereas SRL and RMR was not dominantly influenced by N:P ratio. We conclude that increased N stimulates phosphatase activity via N:P stoichiometry effects, which potentially increases plant P uptake in a species-specific way. N deposition, therefore, may alter plant community structure not only by enhancing productivity, but also by favouring species with traits that enable them to persist better under P limited conditions.

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Contributors

Author: Bjorn J M Robroek

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