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Mammalian PNLDC1 is a novel poly(A) specific exonuclease with discrete expression during early development

Mammalian PNLDC1 is a novel poly(A) specific exonuclease with discrete expression during early development
Mammalian PNLDC1 is a novel poly(A) specific exonuclease with discrete expression during early development

PNLDC1 is a homologue of poly(A) specific ribonuclease (PARN), a known deadenylase with additional role in processing of non-coding RNAs. Both enzymes were reported recently to participate in piRNA biogenesis in silkworm and C. elegans, respectively. To get insights on the role of mammalian PNLDC1, we characterized the human and mouse enzymes. PNLDC1 shows limited conservation compared to PARN and represents an evolutionary related but distinct group of enzymes. It is expressed specifically in mouse embryonic stem cells, human and mouse testes and during early mouse embryo development, while it fades during differentiation. Its expression in differentiated cells, is suppressed through methylation of its promoter by the de novo methyltransferase DNMT3B. Both enzymes are localized mainly in the ER and exhibit in vitro specificity restricted solely to 3' RNA or DNA polyadenylates. Knockdown of Pnldc1 in mESCs and subsequent NGS analysis showed that although the expression of the remaining deadenylases remains unaffected, it affects genes involved mainly in reprogramming, cell cycle and translational regulation. Mammalian PNLDC1 is a novel deadenylase expressed specifically in cell types which share regulatory mechanisms required for multipotency maintenance. Moreover, it could be involved both in posttranscriptional regulation through deadenylation and genome surveillance during early development.

0305-1048
8908-8920
Anastasakis, Dimitrios
3dd90ba5-614c-4efb-83a8-90b5be97a093
Skeparnias, Ilias
ec7ce2f9-312e-492b-b3d9-2af330e21594
Shaukat, Athanasios Nasir
021006f5-ffb9-4dbe-adbd-c18022ec18ec
Grafanaki, Katerina
287b7719-5631-47e7-8ada-be581adad71b
Kanellou, Alexandra
e70ec8f1-9007-450c-b02e-a9c3f96a6c4b
Taraviras, Stavros
d25cadd7-85df-4b72-9e26-e60f84006121
Papachristou, Dionysios J.
9064ad72-ce83-4969-858e-cda0e9cf7275
Papakyriakou, Athanasios
939bc8c9-1693-4530-9099-c55772b22f1d
Stathopoulos, Constantinos
63276407-ccc9-4452-ba11-46d814219a86
Anastasakis, Dimitrios
3dd90ba5-614c-4efb-83a8-90b5be97a093
Skeparnias, Ilias
ec7ce2f9-312e-492b-b3d9-2af330e21594
Shaukat, Athanasios Nasir
021006f5-ffb9-4dbe-adbd-c18022ec18ec
Grafanaki, Katerina
287b7719-5631-47e7-8ada-be581adad71b
Kanellou, Alexandra
e70ec8f1-9007-450c-b02e-a9c3f96a6c4b
Taraviras, Stavros
d25cadd7-85df-4b72-9e26-e60f84006121
Papachristou, Dionysios J.
9064ad72-ce83-4969-858e-cda0e9cf7275
Papakyriakou, Athanasios
939bc8c9-1693-4530-9099-c55772b22f1d
Stathopoulos, Constantinos
63276407-ccc9-4452-ba11-46d814219a86

Anastasakis, Dimitrios, Skeparnias, Ilias, Shaukat, Athanasios Nasir, Grafanaki, Katerina, Kanellou, Alexandra, Taraviras, Stavros, Papachristou, Dionysios J., Papakyriakou, Athanasios and Stathopoulos, Constantinos (2016) Mammalian PNLDC1 is a novel poly(A) specific exonuclease with discrete expression during early development. Nucleic Acids Research, 44 (18), 8908-8920. (doi:10.1093/nar/gkw709).

Record type: Article

Abstract

PNLDC1 is a homologue of poly(A) specific ribonuclease (PARN), a known deadenylase with additional role in processing of non-coding RNAs. Both enzymes were reported recently to participate in piRNA biogenesis in silkworm and C. elegans, respectively. To get insights on the role of mammalian PNLDC1, we characterized the human and mouse enzymes. PNLDC1 shows limited conservation compared to PARN and represents an evolutionary related but distinct group of enzymes. It is expressed specifically in mouse embryonic stem cells, human and mouse testes and during early mouse embryo development, while it fades during differentiation. Its expression in differentiated cells, is suppressed through methylation of its promoter by the de novo methyltransferase DNMT3B. Both enzymes are localized mainly in the ER and exhibit in vitro specificity restricted solely to 3' RNA or DNA polyadenylates. Knockdown of Pnldc1 in mESCs and subsequent NGS analysis showed that although the expression of the remaining deadenylases remains unaffected, it affects genes involved mainly in reprogramming, cell cycle and translational regulation. Mammalian PNLDC1 is a novel deadenylase expressed specifically in cell types which share regulatory mechanisms required for multipotency maintenance. Moreover, it could be involved both in posttranscriptional regulation through deadenylation and genome surveillance during early development.

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Accepted/In Press date: 2 August 2016
e-pub ahead of print date: 11 August 2016
Published date: 14 October 2016

Identifiers

Local EPrints ID: 413894
URI: http://eprints.soton.ac.uk/id/eprint/413894
ISSN: 0305-1048
PURE UUID: 09d4836f-022a-42f2-8744-94be8472c92d
ORCID for Athanasios Papakyriakou: ORCID iD orcid.org/0000-0003-3931-6232

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Date deposited: 08 Sep 2017 16:31
Last modified: 17 Dec 2019 01:28

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