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Fingerprinting polychlorinated biphenyls in environmental samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry

Fingerprinting polychlorinated biphenyls in environmental samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry
Fingerprinting polychlorinated biphenyls in environmental samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry

A GC × GC-TOFMS installed with a Rtx-PCB (60. m × 0.18. mm × 0.18. μm) in the first dimension and Rxi-17 (1.5. m × 0.1. mm × 0.1. μm) column in the second dimension was used to separate 188 out of 209 congeners. A further 12 congeners were identified through additional data processing resulting in the identification of a total of 200 congeners. However, caution is advised if these 12 congeners were to be used in quantitative assessments. The remaining 9 co-eluting congeners were three doublets (CB65. +. CB62, CB160. +. CB163 and CB201. +. CB204) and one triplet (CB20. +. CB21. +. CB33). This method was tested on five Aroclors and resulted in the separation of all congeners present in the heavier Aroclor mixtures A1254 and A1260. The suitability of this method for applications in biological matrices was demonstrated on extracted whiting and guillemot liver samples which resulted in the identification of 137 individual PCBs in the whiting liver sample and 120 in the guillemot sample. Fingerprinting was able to show clear differences in the PCB signature of the two animals. This highlights the potential of this method for PCB fingerprinting in environmental forensics studies and other assessments that require congener specific analysis.

Aroclor, Comprehensive two-dimensional gas chromatography, Polychlorinated biphenyls, Time of flight mass spectrometry
0021-9673
276-283
Megson, David
8f149361-86db-468c-897d-510bf5e470a8
Kalin, Robert
f0707b24-82db-4a48-a873-98666499d141
Worsfold, Paul J.
27675f89-7eee-45c5-821e-a381d8db9693
Gauchotte-Lindsay, Caroline
49baa8a7-b464-4a10-afe4-de0fd8e64349
Patterson, Donald G.
78fb7d17-68e4-45fc-b980-9384fe05f829
Lohan, Maeve C.
6ca10597-2d0f-40e8-8e4f-7619dfac5088
Comber, Sean
cd5302d9-9c84-418f-8475-10e46eb059a4
Brown, Thomas A.
6eaa01b9-51e7-4c63-9d9d-172b93975600
O'Sullivan, Gwen
32754a23-e3fc-47c8-9cec-005349967592
Megson, David
8f149361-86db-468c-897d-510bf5e470a8
Kalin, Robert
f0707b24-82db-4a48-a873-98666499d141
Worsfold, Paul J.
27675f89-7eee-45c5-821e-a381d8db9693
Gauchotte-Lindsay, Caroline
49baa8a7-b464-4a10-afe4-de0fd8e64349
Patterson, Donald G.
78fb7d17-68e4-45fc-b980-9384fe05f829
Lohan, Maeve C.
6ca10597-2d0f-40e8-8e4f-7619dfac5088
Comber, Sean
cd5302d9-9c84-418f-8475-10e46eb059a4
Brown, Thomas A.
6eaa01b9-51e7-4c63-9d9d-172b93975600
O'Sullivan, Gwen
32754a23-e3fc-47c8-9cec-005349967592

Megson, David, Kalin, Robert, Worsfold, Paul J., Gauchotte-Lindsay, Caroline, Patterson, Donald G., Lohan, Maeve C., Comber, Sean, Brown, Thomas A. and O'Sullivan, Gwen (2013) Fingerprinting polychlorinated biphenyls in environmental samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry. Journal of Chromatography A, 1318, 276-283. (doi:10.1016/j.chroma.2013.10.016).

Record type: Article

Abstract

A GC × GC-TOFMS installed with a Rtx-PCB (60. m × 0.18. mm × 0.18. μm) in the first dimension and Rxi-17 (1.5. m × 0.1. mm × 0.1. μm) column in the second dimension was used to separate 188 out of 209 congeners. A further 12 congeners were identified through additional data processing resulting in the identification of a total of 200 congeners. However, caution is advised if these 12 congeners were to be used in quantitative assessments. The remaining 9 co-eluting congeners were three doublets (CB65. +. CB62, CB160. +. CB163 and CB201. +. CB204) and one triplet (CB20. +. CB21. +. CB33). This method was tested on five Aroclors and resulted in the separation of all congeners present in the heavier Aroclor mixtures A1254 and A1260. The suitability of this method for applications in biological matrices was demonstrated on extracted whiting and guillemot liver samples which resulted in the identification of 137 individual PCBs in the whiting liver sample and 120 in the guillemot sample. Fingerprinting was able to show clear differences in the PCB signature of the two animals. This highlights the potential of this method for PCB fingerprinting in environmental forensics studies and other assessments that require congener specific analysis.

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More information

Published date: 29 November 2013
Keywords: Aroclor, Comprehensive two-dimensional gas chromatography, Polychlorinated biphenyls, Time of flight mass spectrometry

Identifiers

Local EPrints ID: 414024
URI: http://eprints.soton.ac.uk/id/eprint/414024
ISSN: 0021-9673
PURE UUID: e9806aa9-7660-4185-a093-f452df39b923
ORCID for Maeve C. Lohan: ORCID iD orcid.org/0000-0002-5340-3108

Catalogue record

Date deposited: 12 Sep 2017 16:32
Last modified: 16 Mar 2024 04:13

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Contributors

Author: David Megson
Author: Robert Kalin
Author: Paul J. Worsfold
Author: Caroline Gauchotte-Lindsay
Author: Donald G. Patterson
Author: Maeve C. Lohan ORCID iD
Author: Sean Comber
Author: Thomas A. Brown
Author: Gwen O'Sullivan

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