Glycan microheterogeneity at the PGT135 antibody recognition site on HIV-1 gp120 reveals a molecular mechanism for neutralization resistance
Glycan microheterogeneity at the PGT135 antibody recognition site on HIV-1 gp120 reveals a molecular mechanism for neutralization resistance
Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan microheterogeneity sufficient to explain the existence of a minor population of virions resistant to PGT135 neutralization. Target microheterogeneity and antibody glycan specificity are therefore important parameters in HIV-1 vaccine design.
6952-6959
Pritchard, Laura K.
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Spencer, Daniel I.R.
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Royle, Louise
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Vasiljevic, Snezana
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Krumm, Stefanie A.
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Doores, Katie J.
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Crispin, Matthew
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2015
Pritchard, Laura K.
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Spencer, Daniel I.R.
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Royle, Louise
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Vasiljevic, Snezana
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Krumm, Stefanie A.
534eb807-ab99-4eef-984a-c26dc5028c9e
Doores, Katie J.
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Crispin, Matthew
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Pritchard, Laura K., Spencer, Daniel I.R., Royle, Louise, Vasiljevic, Snezana, Krumm, Stefanie A., Doores, Katie J. and Crispin, Matthew
(2015)
Glycan microheterogeneity at the PGT135 antibody recognition site on HIV-1 gp120 reveals a molecular mechanism for neutralization resistance.
Journal of Virology, 89 (13), .
(doi:10.1128/JVI.00230-15).
Abstract
Broadly neutralizing antibodies have been isolated that bind the glycan shield of the HIV-1 envelope spike. One such antibody, PGT135, contacts the intrinsic mannose patch of gp120 at the Asn332, Asn392, and Asn386 glycosylation sites. Here, site-specific glycosylation analysis of recombinant gp120 revealed glycan microheterogeneity sufficient to explain the existence of a minor population of virions resistant to PGT135 neutralization. Target microheterogeneity and antibody glycan specificity are therefore important parameters in HIV-1 vaccine design.
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Published date: 2015
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Local EPrints ID: 414267
URI: http://eprints.soton.ac.uk/id/eprint/414267
ISSN: 0022-538X
PURE UUID: 6ba58ba9-dc91-4f34-8441-9c403c0c9594
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Date deposited: 21 Sep 2017 16:31
Last modified: 16 Mar 2024 04:30
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Contributors
Author:
Laura K. Pritchard
Author:
Daniel I.R. Spencer
Author:
Louise Royle
Author:
Snezana Vasiljevic
Author:
Stefanie A. Krumm
Author:
Katie J. Doores
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