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Evidence for involvement of IL-9 and IL-22 in cows’ milk allergy in infants

Evidence for involvement of IL-9 and IL-22 in cows’ milk allergy in infants
Evidence for involvement of IL-9 and IL-22 in cows’ milk allergy in infants
Although allergic inflammation is characterized by a T helper (Th) 2-dominant immune response, the discovery of a role for new T cell subsets in inflammatory diseases has added an additional layer of complexity to the understanding of the pathogeneses of allergic diseases. We evaluated plasma cytokine profiles in infants with cows’ milk allergy (CMA), who were being treated with an elimination diet. In a prospective, randomized and controlled study, infants (aged 8.4 ± 3.9 months) with CMA were treated with an elimination diet for 120 days, which replaced cows’ milk with a hydrolysed soy protein formula (n = 26) or a free amino acid formula (n = 20). Blood samples were collected before treatment during active disease (T0) and after 120 days, when symptoms were absent (T1). Plasma cytokine concentrations were measured. Infants with CMA had higher plasma concentrations of interleukin (IL)-4 and IL-13 and lower concentrations of IL-9, IL-17A and interferon-γ, compared with healthy breast-fed infants. At T0, there was a positive correlation between blood eosinophil numbers and plasma concentrations of IL-4, IL-9, IL-17A and IL-22. Treatment with a cows’ milk elimination diet resulted in a decrease in plasma IL-4, IL-9, IL-13 and IL-22 and an increase in plasma IL-17A. We conclude that IL-4 and IL-13 are elevated in active CMA. The association of IL-9 and IL-22 with eosinophilia, and the decrease in these two cytokines with cows’ milk elimination, suggests that they both play a role in the symptoms observed in CMA and may be important targets for future interventions
Barros, Karina V.
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Flor Silveira, Vera L.
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Laranjeira, Marisa S.
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Wandalsen, Neusa F.
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Passeti, Susana
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de Oliveira, Roberta
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Munekata, Regina V.
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Noakes, Paul S.
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Miles, Elizabeth A.
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Calder, Philip C.
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Barros, Karina V.
ed8d7721-2f0b-4a70-81d7-ed7d46754cf1
Flor Silveira, Vera L.
0e87b085-04a7-4cda-80fe-f0c3bdb10f9a
Laranjeira, Marisa S.
fb99ae99-ea31-4f35-b2ee-0a83d3f4d5ca
Wandalsen, Neusa F.
46d3862a-9004-43d9-902b-f26351c78851
Passeti, Susana
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de Oliveira, Roberta
ac204bf7-eb62-4e7f-88ba-4cc420b38443
Munekata, Regina V.
deef4e35-b1eb-413f-a504-9e053adb93a8
Noakes, Paul S.
0b1ef763-d63e-4550-805d-d76022b4aa87
Miles, Elizabeth A.
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Calder, Philip C.
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Barros, Karina V., Flor Silveira, Vera L., Laranjeira, Marisa S., Wandalsen, Neusa F., Passeti, Susana, de Oliveira, Roberta, Munekata, Regina V., Noakes, Paul S., Miles, Elizabeth A. and Calder, Philip C. (2017) Evidence for involvement of IL-9 and IL-22 in cows’ milk allergy in infants. Nutrients, 9 (10). (doi:10.3390/nu9101048).

Record type: Article

Abstract

Although allergic inflammation is characterized by a T helper (Th) 2-dominant immune response, the discovery of a role for new T cell subsets in inflammatory diseases has added an additional layer of complexity to the understanding of the pathogeneses of allergic diseases. We evaluated plasma cytokine profiles in infants with cows’ milk allergy (CMA), who were being treated with an elimination diet. In a prospective, randomized and controlled study, infants (aged 8.4 ± 3.9 months) with CMA were treated with an elimination diet for 120 days, which replaced cows’ milk with a hydrolysed soy protein formula (n = 26) or a free amino acid formula (n = 20). Blood samples were collected before treatment during active disease (T0) and after 120 days, when symptoms were absent (T1). Plasma cytokine concentrations were measured. Infants with CMA had higher plasma concentrations of interleukin (IL)-4 and IL-13 and lower concentrations of IL-9, IL-17A and interferon-γ, compared with healthy breast-fed infants. At T0, there was a positive correlation between blood eosinophil numbers and plasma concentrations of IL-4, IL-9, IL-17A and IL-22. Treatment with a cows’ milk elimination diet resulted in a decrease in plasma IL-4, IL-9, IL-13 and IL-22 and an increase in plasma IL-17A. We conclude that IL-4 and IL-13 are elevated in active CMA. The association of IL-9 and IL-22 with eosinophilia, and the decrease in these two cytokines with cows’ milk elimination, suggests that they both play a role in the symptoms observed in CMA and may be important targets for future interventions

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Accepted/In Press date: 18 September 2017
e-pub ahead of print date: 21 September 2017
Published date: 21 September 2017

Identifiers

Local EPrints ID: 414297
URI: https://eprints.soton.ac.uk/id/eprint/414297
PURE UUID: 9a7e82ac-dfc3-4570-9aec-e7c127ac2aa4
ORCID for Elizabeth A. Miles: ORCID iD orcid.org/0000-0002-8643-0655

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Date deposited: 26 Sep 2017 16:30
Last modified: 17 Jul 2019 01:20

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Contributors

Author: Karina V. Barros
Author: Vera L. Flor Silveira
Author: Marisa S. Laranjeira
Author: Neusa F. Wandalsen
Author: Susana Passeti
Author: Roberta de Oliveira
Author: Regina V. Munekata
Author: Paul S. Noakes
Author: Elizabeth A. Miles ORCID iD

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