Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies

Pritchard, Laura K., Spencer, Daniel I.R., Royle, Louise, Bonomelli, Camille, Seabright, Gemma E., Behrens, Anna Janina, Kulp, Daniel W., Menis, Sergey, Krumm, Stefanie A., Dunlop, D. Cameron, Crispin, Daniel J., Bowden, Thomas A., Scanlan, Christopher N., Ward, Andrew B., Schief, William R., Doores, Katie J. and Crispin, Max (2015) Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies. Nature Communications, 6, [7449]. (doi:10.1038/ncomms8479).

Abstract

The envelope spike of HIV-1 employs a 'glycan shield' to protect itself from antibody-mediated neutralization. Paradoxically, however, potent broadly neutralizing antibodies (bnAbs) that target this shield have been isolated. The unusually high glycan density on the gp120 subunit limits processing during biosynthesis, leaving a region of under-processed oligomannose-type structures, which is a primary target of these bnAbs. Here we investigate the contribution of individual glycosylation sites in the formation of this so-called intrinsic mannose patch. Deletion of individual sites has a limited effect on the overall size of the intrinsic mannose patch but leads to changes in the processing of neighbouring glycans. These structural changes are largely tolerated by a panel of glycan-dependent bnAbs targeting these regions, indicating a degree of plasticity in their recognition. These results support the intrinsic mannose patch as a stable target for vaccine design.

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Contributors

Author: Max Crispin

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