The University of Southampton
×
Filter your search:
University of Southampton Institutional Repository

Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility

Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility
Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility

Glycan analysis of virion-derived glycoproteins is challenging due to the diffi culties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

Glycoprotein, Glycosylation, Mass spectrometry, Structure, Virus
93-121
Springer New York, NY
Bitto, David
e21f1990-512d-48b4-8945-09201788bb13
Harvey, David J.
8bb24417-3852-4b1f-827b-0d5d2c176744
Halldorsson, Steinar
7bf94dad-782a-4f02-907c-06361efb2230
Doores, Katie J.
52d36150-7a62-4f9d-8348-c83a789d52e6
Pritchard, Laura K.
bfa1d1b4-50b6-401f-b153-8c3322b2e726
Huiskonen, Juha T.
75da2956-19f1-427c-8ae6-4b6df68f79c0
Bowden, Thomas A.
4b17a588-ac01-4112-807a-8b99a6c20d0f
Crispin, Matthew
cd980957-0943-4b89-b2b2-710f01f33bc9
Bitto, David
e21f1990-512d-48b4-8945-09201788bb13
Harvey, David J.
8bb24417-3852-4b1f-827b-0d5d2c176744
Halldorsson, Steinar
7bf94dad-782a-4f02-907c-06361efb2230
Doores, Katie J.
52d36150-7a62-4f9d-8348-c83a789d52e6
Pritchard, Laura K.
bfa1d1b4-50b6-401f-b153-8c3322b2e726
Huiskonen, Juha T.
75da2956-19f1-427c-8ae6-4b6df68f79c0
Bowden, Thomas A.
4b17a588-ac01-4112-807a-8b99a6c20d0f
Crispin, Matthew
cd980957-0943-4b89-b2b2-710f01f33bc9

Bitto, David, Harvey, David J., Halldorsson, Steinar, Doores, Katie J., Pritchard, Laura K., Huiskonen, Juha T., Bowden, Thomas A. and Crispin, Matthew (2015) Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility. In, Carbohydrate-Based Vaccines: Methods and Protocols. Springer New York, NY, pp. 93-121. (doi:10.1007/978-1-4939-2874-3_7).

Record type: Book Section

Abstract

Glycan analysis of virion-derived glycoproteins is challenging due to the diffi culties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

Text
Bitto_MethodsMolBiol_2015_Accepted - Accepted Manuscript
Download (1MB)

More information

Published date: 13 July 2015
Related URLs:
Keywords: Glycoprotein, Glycosylation, Mass spectrometry, Structure, Virus

Identifiers

Local EPrints ID: 414429
URI: http://eprints.soton.ac.uk/id/eprint/414429
DOI: doi:10.1007/978-1-4939-2874-3_7
PURE UUID: 0bc1ef19-1c36-4b74-80ea-0c08d0fbb354
ORCID for Matthew Crispin: ORCID iD orcid.org/0000-0002-1072-2694

Catalogue record

Date deposited: 28 Sep 2017 16:31
Last modified: 16 Mar 2024 04:30

Export record

Altmetrics

Contributors

Author: David Bitto
Author: David J. Harvey
Author: Steinar Halldorsson
Author: Katie J. Doores
Author: Laura K. Pritchard
Author: Juha T. Huiskonen
Author: Thomas A. Bowden
Author: Matthew Crispin ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Library staff additional information
Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×