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Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility

Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility
Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility

Glycan analysis of virion-derived glycoproteins is challenging due to the diffi culties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

Glycoprotein, Glycosylation, Mass spectrometry, Structure, Virus
93-121
Springer New York
Bitto, David
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Harvey, David J.
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Halldorsson, Steinar
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Doores, Katie J.
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Pritchard, Laura K.
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Huiskonen, Juha T.
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Bowden, Thomas A.
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Crispin, Matthew
cd980957-0943-4b89-b2b2-710f01f33bc9
Bitto, David
e21f1990-512d-48b4-8945-09201788bb13
Harvey, David J.
8bb24417-3852-4b1f-827b-0d5d2c176744
Halldorsson, Steinar
7bf94dad-782a-4f02-907c-06361efb2230
Doores, Katie J.
52d36150-7a62-4f9d-8348-c83a789d52e6
Pritchard, Laura K.
bfa1d1b4-50b6-401f-b153-8c3322b2e726
Huiskonen, Juha T.
75da2956-19f1-427c-8ae6-4b6df68f79c0
Bowden, Thomas A.
4b17a588-ac01-4112-807a-8b99a6c20d0f
Crispin, Matthew
cd980957-0943-4b89-b2b2-710f01f33bc9

Bitto, David, Harvey, David J., Halldorsson, Steinar, Doores, Katie J., Pritchard, Laura K., Huiskonen, Juha T., Bowden, Thomas A. and Crispin, Matthew (2015) Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility. In, Carbohydrate-Based Vaccines: Methods and Protocols. Springer New York, pp. 93-121. (doi:10.1007/978-1-4939-2874-3_7).

Record type: Book Section

Abstract

Glycan analysis of virion-derived glycoproteins is challenging due to the diffi culties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

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Bitto_MethodsMolBiol_2015_Accepted - Accepted Manuscript
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Published date: 13 July 2015
Keywords: Glycoprotein, Glycosylation, Mass spectrometry, Structure, Virus

Identifiers

Local EPrints ID: 414429
URI: https://eprints.soton.ac.uk/id/eprint/414429
PURE UUID: 0bc1ef19-1c36-4b74-80ea-0c08d0fbb354
ORCID for Matthew Crispin: ORCID iD orcid.org/0000-0002-1072-2694

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Date deposited: 28 Sep 2017 16:31
Last modified: 03 Apr 2019 00:24

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