Disruption of α-mannosidase processing induces non-canonical hybrid-type glycosylation

Crispin, Max, Aricescu, A. Radu, Chang, Veronica T., Jones, E. Yvonne, Stuart, David I., Dwek, Raymond A., Davis, Simon J. and Harvey, David J. (2007) Disruption of α-mannosidase processing induces non-canonical hybrid-type glycosylation. FEBS Letters, 581 (10), 1963-1968. (doi:10.1016/j.febslet.2007.04.020).

Abstract

Golgi α-mannosidase II is essential for the efficient formation of complex-type glycosylation. Here, we demonstrate that the disruption of Golgi α-mannosidase II activity by swainsonine in human embryonic kidney cells is capable of inducing a novel class of hybrid-type glycosylation containing a partially processed mannose moiety. The discovery of 'Man₆-based' hybrid-type glycans reveals a broader in vivo specificity of N-acetylglucosaminyltransferase I, further defines the arm-specific tolerance of core α1-6 fucosyltransferase to terminal α1-2 mannose residues, and suggests that disruption of Golgi α-mannosidase II activity is capable of inducing potentially 'non-self' structures.

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Contributors

Author: Max Crispin

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