Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions
Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions
Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.
antibody, antibody engineering, glycosylation, kifunensine, oligomannose
1061-1066
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Bowden, Thomas A.
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Coles, Charlotte H.
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Harlos, Karl
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Aricescu, A. Radu
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Harvey, David J.
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Stuart, David I.
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Jones, E. Yvonne
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17 April 2009
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Bowden, Thomas A.
4b17a588-ac01-4112-807a-8b99a6c20d0f
Coles, Charlotte H.
8c3ba446-999e-431a-8a4c-aab4ccd3b2dc
Harlos, Karl
b5f7d1ec-d765-4d39-b8f2-65c6917f1d21
Aricescu, A. Radu
8ee3d2bd-7ef7-4e85-862a-e13f48ae0fa6
Harvey, David J.
8bb24417-3852-4b1f-827b-0d5d2c176744
Stuart, David I.
2751c230-9d4c-4981-aeb7-cafb51ed749d
Jones, E. Yvonne
6b9004c9-137b-4f43-8a14-c7c83bcaa4be
Crispin, Max, Bowden, Thomas A., Coles, Charlotte H., Harlos, Karl, Aricescu, A. Radu, Harvey, David J., Stuart, David I. and Jones, E. Yvonne
(2009)
Carbohydrate and domain architecture of an immature antibody glycoform exhibiting enhanced effector functions.
Journal of Molecular Biology, 387 (5), .
(doi:10.1016/j.jmb.2009.02.033).
Abstract
Antibodies contain a conserved glycosylation site that has emerged as a target for the modulation of antibody effector functions. The crystal structure of a biosynthetic intermediate of human IgG1, bearing immature oligomannose-type glycans and reported to display increased antibody-dependent cellular cytotoxicity, demonstrates that glycan engineering can bias the Fc to an open conformation primed for receptor binding.
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Published date: 17 April 2009
Keywords:
antibody, antibody engineering, glycosylation, kifunensine, oligomannose
Identifiers
Local EPrints ID: 414586
URI: http://eprints.soton.ac.uk/id/eprint/414586
ISSN: 0022-2836
PURE UUID: 36463085-f997-45e0-8159-985cf2a14b04
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Date deposited: 04 Oct 2017 16:30
Last modified: 16 Mar 2024 04:30
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Contributors
Author:
Thomas A. Bowden
Author:
Charlotte H. Coles
Author:
Karl Harlos
Author:
A. Radu Aricescu
Author:
David J. Harvey
Author:
David I. Stuart
Author:
E. Yvonne Jones
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