The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade
The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade
The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associated gp120 from primary virus from PBMCs for a range of viral isolates and showed cross-clade elevation (62-79%) of these glycans relative to recombinant, monomeric gp120 (~30%). We also confirmed that pseudoviral production systems can give rise to notably elevated gp120 oligomannose levels (~98%), compared to gp120 derived from a single-plasmid viral system using the HIV LAI backbone (56%). This study highlights differences in glycosylation between virion-associated and recombinant gp120.
Bonomelli, Camille
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Doores, Katie J.
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Dunlop, D. Cameron
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Thaney, Victoria
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Dwek, Raymond A.
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Burton, Dennis R.
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Crispin, Max
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Scanlan, Christopher N.
04dd1b57-b6fc-414c-8595-08310dbb3d32
2011
Bonomelli, Camille
51edb32c-85d0-45be-b050-b075cc3f6c28
Doores, Katie J.
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Dunlop, D. Cameron
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Thaney, Victoria
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Dwek, Raymond A.
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Burton, Dennis R.
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Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Scanlan, Christopher N.
04dd1b57-b6fc-414c-8595-08310dbb3d32
Bonomelli, Camille, Doores, Katie J., Dunlop, D. Cameron, Thaney, Victoria, Dwek, Raymond A., Burton, Dennis R., Crispin, Max and Scanlan, Christopher N.
(2011)
The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade.
PLoS ONE, 6 (8), [e23521].
(doi:10.1371/journal.pone.0023521).
Abstract
The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associated gp120 from primary virus from PBMCs for a range of viral isolates and showed cross-clade elevation (62-79%) of these glycans relative to recombinant, monomeric gp120 (~30%). We also confirmed that pseudoviral production systems can give rise to notably elevated gp120 oligomannose levels (~98%), compared to gp120 derived from a single-plasmid viral system using the HIV LAI backbone (56%). This study highlights differences in glycosylation between virion-associated and recombinant gp120.
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Published date: 2011
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Local EPrints ID: 414593
URI: http://eprints.soton.ac.uk/id/eprint/414593
ISSN: 1932-6203
PURE UUID: 05de7519-0368-4dbb-bcae-a7ee4fe0a1b3
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Date deposited: 04 Oct 2017 16:30
Last modified: 06 Jun 2024 01:59
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Author:
Camille Bonomelli
Author:
Katie J. Doores
Author:
D. Cameron Dunlop
Author:
Victoria Thaney
Author:
Raymond A. Dwek
Author:
Dennis R. Burton
Author:
Christopher N. Scanlan
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