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The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade

The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade
The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade

The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associated gp120 from primary virus from PBMCs for a range of viral isolates and showed cross-clade elevation (62-79%) of these glycans relative to recombinant, monomeric gp120 (~30%). We also confirmed that pseudoviral production systems can give rise to notably elevated gp120 oligomannose levels (~98%), compared to gp120 derived from a single-plasmid viral system using the HIV LAI backbone (56%). This study highlights differences in glycosylation between virion-associated and recombinant gp120.

1932-6203
Bonomelli, Camille
51edb32c-85d0-45be-b050-b075cc3f6c28
Doores, Katie J.
52d36150-7a62-4f9d-8348-c83a789d52e6
Dunlop, D. Cameron
2d74525d-665a-40c0-97c3-5b61e1241f94
Thaney, Victoria
642c4f13-7cab-4156-935d-e7fed0281718
Dwek, Raymond A.
d8d9d5f8-f2c0-414e-b6b0-df77a33f0da4
Burton, Dennis R.
a628ce77-b694-4e3c-86a5-11f4e20e1c7c
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Scanlan, Christopher N.
04dd1b57-b6fc-414c-8595-08310dbb3d32
Bonomelli, Camille
51edb32c-85d0-45be-b050-b075cc3f6c28
Doores, Katie J.
52d36150-7a62-4f9d-8348-c83a789d52e6
Dunlop, D. Cameron
2d74525d-665a-40c0-97c3-5b61e1241f94
Thaney, Victoria
642c4f13-7cab-4156-935d-e7fed0281718
Dwek, Raymond A.
d8d9d5f8-f2c0-414e-b6b0-df77a33f0da4
Burton, Dennis R.
a628ce77-b694-4e3c-86a5-11f4e20e1c7c
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Scanlan, Christopher N.
04dd1b57-b6fc-414c-8595-08310dbb3d32

Bonomelli, Camille, Doores, Katie J., Dunlop, D. Cameron, Thaney, Victoria, Dwek, Raymond A., Burton, Dennis R., Crispin, Max and Scanlan, Christopher N. (2011) The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade. PLoS ONE, 6 (8). (doi:10.1371/journal.pone.0023521).

Record type: Article

Abstract

The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associated gp120 from primary virus from PBMCs for a range of viral isolates and showed cross-clade elevation (62-79%) of these glycans relative to recombinant, monomeric gp120 (~30%). We also confirmed that pseudoviral production systems can give rise to notably elevated gp120 oligomannose levels (~98%), compared to gp120 derived from a single-plasmid viral system using the HIV LAI backbone (56%). This study highlights differences in glycosylation between virion-associated and recombinant gp120.

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Published date: 2011

Identifiers

Local EPrints ID: 414593
URI: https://eprints.soton.ac.uk/id/eprint/414593
ISSN: 1932-6203
PURE UUID: 05de7519-0368-4dbb-bcae-a7ee4fe0a1b3
ORCID for Max Crispin: ORCID iD orcid.org/0000-0002-1072-2694

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Date deposited: 04 Oct 2017 16:30
Last modified: 14 Mar 2019 01:25

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