The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.)
The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.)
The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccharides. Considerable heterogeneity was observed. Sixteen different glycan structures were identified, over 60% were sialylated and 40% contained core fucose. The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody.
Carbohydrate moiety, Cod (Gadus morhua L.), Immunoglobulin IgM, Oligosaccharide sequencing
209-227
Magnadóttir, Bergljót
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Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Royle, Louise
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Colominas, Cristina
4bf2f298-01cd-4855-81f5-f50c4212cb4f
Harvey, David J.
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Dwek, Raymond A.
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Rudd, Pauline M.
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2002
Magnadóttir, Bergljót
80f8e325-120e-4808-aaf8-d37e37f8a44e
Crispin, Max
cd980957-0943-4b89-b2b2-710f01f33bc9
Royle, Louise
310a52f9-a086-482c-9963-857a3af2363b
Colominas, Cristina
4bf2f298-01cd-4855-81f5-f50c4212cb4f
Harvey, David J.
a8e787b1-801c-4e66-b5a1-6265570b519d
Dwek, Raymond A.
d8d9d5f8-f2c0-414e-b6b0-df77a33f0da4
Rudd, Pauline M.
4bbd1e70-98ae-4c28-84e6-c6a18d98e7ee
Magnadóttir, Bergljót, Crispin, Max, Royle, Louise, Colominas, Cristina, Harvey, David J., Dwek, Raymond A. and Rudd, Pauline M.
(2002)
The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.).
Fish and Shellfish Immunology, 12 (3), .
(doi:10.1006/fsim.2001.0364).
Abstract
The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccharides. Considerable heterogeneity was observed. Sixteen different glycan structures were identified, over 60% were sialylated and 40% contained core fucose. The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody.
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Published date: 2002
Keywords:
Carbohydrate moiety, Cod (Gadus morhua L.), Immunoglobulin IgM, Oligosaccharide sequencing
Identifiers
Local EPrints ID: 414688
URI: http://eprints.soton.ac.uk/id/eprint/414688
ISSN: 1050-4648
PURE UUID: 9ae09a50-db69-4f1f-83e1-30f4f8accb90
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Date deposited: 05 Oct 2017 16:31
Last modified: 16 Mar 2024 04:30
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Contributors
Author:
Bergljót Magnadóttir
Author:
Louise Royle
Author:
Cristina Colominas
Author:
David J. Harvey
Author:
Raymond A. Dwek
Author:
Pauline M. Rudd
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