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Versatile magnetic resonance singlet tags compatible with biological conditions

Versatile magnetic resonance singlet tags compatible with biological conditions
Versatile magnetic resonance singlet tags compatible with biological conditions
The long lifetime of nuclear singlet states holds promise for the development of molecular tracers to study motional processes in proteins with increased precision or to act as imaging contrast agents. We introduce a singlet tag (STAG) based on bromoacrylate that is readily attached to a variety of biomolecules, compatible with biological conditions, and is relatively insensitive to the presence of molecular oxygen. The generality of this approach is demonstrated by tagging buildings blocks for biomolecules using a simple labelling route based on commercially available starting materials. Additionally, we demonstrate a singlet NMR experiment on the endogenous molecule phosphoenolpyruvate.
2046-2069
34574-34578
Gloggler, Stefan
e6b46e9a-8594-4c5a-bced-e2c2f946c390
Elliott, Stuart
6cc724de-f75f-4c0f-a00a-e712b4e73b12
Stevanato, Gabriele
4e8bed5e-b597-4dea-9a8b-5bf886bf8a18
Brown, Richard C.D.
21ce697a-7c3a-480e-919f-429a3d8550f5
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Gloggler, Stefan
e6b46e9a-8594-4c5a-bced-e2c2f946c390
Elliott, Stuart
6cc724de-f75f-4c0f-a00a-e712b4e73b12
Stevanato, Gabriele
4e8bed5e-b597-4dea-9a8b-5bf886bf8a18
Brown, Richard C.D.
21ce697a-7c3a-480e-919f-429a3d8550f5
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3

Gloggler, Stefan, Elliott, Stuart, Stevanato, Gabriele, Brown, Richard C.D. and Levitt, Malcolm H. (2017) Versatile magnetic resonance singlet tags compatible with biological conditions. RSC Advances, 7 (55), 34574-34578. (doi:10.1039/C7RA05196D).

Record type: Article

Abstract

The long lifetime of nuclear singlet states holds promise for the development of molecular tracers to study motional processes in proteins with increased precision or to act as imaging contrast agents. We introduce a singlet tag (STAG) based on bromoacrylate that is readily attached to a variety of biomolecules, compatible with biological conditions, and is relatively insensitive to the presence of molecular oxygen. The generality of this approach is demonstrated by tagging buildings blocks for biomolecules using a simple labelling route based on commercially available starting materials. Additionally, we demonstrate a singlet NMR experiment on the endogenous molecule phosphoenolpyruvate.

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Accepted/In Press date: 30 June 2017
e-pub ahead of print date: 10 July 2017
Published date: 2017

Identifiers

Local EPrints ID: 414773
URI: http://eprints.soton.ac.uk/id/eprint/414773
ISSN: 2046-2069
PURE UUID: 9eafa2f6-40a1-4f52-b88a-cf1330af9b26
ORCID for Richard C.D. Brown: ORCID iD orcid.org/0000-0003-0156-7087
ORCID for Malcolm H. Levitt: ORCID iD orcid.org/0000-0001-9878-1180

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Date deposited: 10 Oct 2017 16:31
Last modified: 18 Feb 2021 16:56

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Contributors

Author: Stefan Gloggler
Author: Stuart Elliott
Author: Gabriele Stevanato

University divisions

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