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Hyperpolarized singlet lifetimes of pyruvate in human blood and in the mouse

Hyperpolarized singlet lifetimes of pyruvate in human blood and in the mouse
Hyperpolarized singlet lifetimes of pyruvate in human blood and in the mouse
Hyperpolarized NMR is a promising technique for non-invasive imaging of tissue metabolism in vivo. However, the pathways that can be studied are limited by the fast T1 decay of the nuclear spin order. In metabolites containing pairs of coupled nuclear spins-1/2, the spin order may be maintained by exploiting the non-magnetic singlet (spin-0) state of the pair. This may allow preservation of the hyperpolarization in vivo during transport to tissues of interest, such as tumors, or to detect slower metabolic reactions. We show here that in human blood and in a mouse in vivo at millitesla fields the 13C singlet lifetime of [1,2-13C2]pyruvate was significantly longer than the 13C T1, although it was shorter than the T1 at field strengths of several tesla. We also examine the singlet-derived NMR spectrum observed for hyperpolarized [1,2-13C2]lactate, originating from the metabolism of [1,2-13C2]pyruvate.
blood, dynamic nuclear polarization, pyruvate, lactate, long-lived states, longitudinal relaxation time, relaxation, hyperpolarization
0127-5720
1696-1704
Marco-Rius, Irene
29f521b5-b4b5-4948-a7ed-0f277c5e056f
Tayler, Michael C. D.
90ef6310-42bb-44fa-9925-e483b931ec99
Kettunen, Mikko I.
cf0f0400-9ab2-4fa6-a98c-fe466efb047e
Larkin, Timothy J.
f63013f0-a86f-4bf7-a9d6-6420be793f93
Timm, Kerstin N.
5e08285f-b787-4700-988e-57e2026bd505
Serrao, Eva M.
3a4ee28c-b27f-44e9-97fb-73b5b0dc78b7
Rodrigues, Tiago B.
81d395c1-c552-4e99-bb8d-6c7872b387a9
Pileio, Giuseppe
13f78e66-0707-4438-b9c9-6dbd3eb7d4e8
Ardenkjaer-Larsen, Jan Henrik
2b4461b1-d845-4363-a259-bb02ea86ecae
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Brindle, Kevin M.
316bceba-bd08-4979-89b3-28d35adf882a
Marco-Rius, Irene
29f521b5-b4b5-4948-a7ed-0f277c5e056f
Tayler, Michael C. D.
90ef6310-42bb-44fa-9925-e483b931ec99
Kettunen, Mikko I.
cf0f0400-9ab2-4fa6-a98c-fe466efb047e
Larkin, Timothy J.
f63013f0-a86f-4bf7-a9d6-6420be793f93
Timm, Kerstin N.
5e08285f-b787-4700-988e-57e2026bd505
Serrao, Eva M.
3a4ee28c-b27f-44e9-97fb-73b5b0dc78b7
Rodrigues, Tiago B.
81d395c1-c552-4e99-bb8d-6c7872b387a9
Pileio, Giuseppe
13f78e66-0707-4438-b9c9-6dbd3eb7d4e8
Ardenkjaer-Larsen, Jan Henrik
2b4461b1-d845-4363-a259-bb02ea86ecae
Levitt, Malcolm H.
bcc5a80a-e5c5-4e0e-9a9a-249d036747c3
Brindle, Kevin M.
316bceba-bd08-4979-89b3-28d35adf882a

Marco-Rius, Irene, Tayler, Michael C. D., Kettunen, Mikko I., Larkin, Timothy J., Timm, Kerstin N., Serrao, Eva M., Rodrigues, Tiago B., Pileio, Giuseppe, Ardenkjaer-Larsen, Jan Henrik, Levitt, Malcolm H. and Brindle, Kevin M. (2013) Hyperpolarized singlet lifetimes of pyruvate in human blood and in the mouse. Tropical Biomedicine, 26 (12), 1696-1704. (doi:10.1002/nbm.3005).

Record type: Article

Abstract

Hyperpolarized NMR is a promising technique for non-invasive imaging of tissue metabolism in vivo. However, the pathways that can be studied are limited by the fast T1 decay of the nuclear spin order. In metabolites containing pairs of coupled nuclear spins-1/2, the spin order may be maintained by exploiting the non-magnetic singlet (spin-0) state of the pair. This may allow preservation of the hyperpolarization in vivo during transport to tissues of interest, such as tumors, or to detect slower metabolic reactions. We show here that in human blood and in a mouse in vivo at millitesla fields the 13C singlet lifetime of [1,2-13C2]pyruvate was significantly longer than the 13C T1, although it was shorter than the T1 at field strengths of several tesla. We also examine the singlet-derived NMR spectrum observed for hyperpolarized [1,2-13C2]lactate, originating from the metabolism of [1,2-13C2]pyruvate.

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More information

Published date: 15 August 2013
Keywords: blood, dynamic nuclear polarization, pyruvate, lactate, long-lived states, longitudinal relaxation time, relaxation, hyperpolarization

Identifiers

Local EPrints ID: 414794
URI: https://eprints.soton.ac.uk/id/eprint/414794
ISSN: 0127-5720
PURE UUID: 3bd2f56d-7f60-4b20-820d-8817a37fca4c
ORCID for Giuseppe Pileio: ORCID iD orcid.org/0000-0001-9223-3896
ORCID for Malcolm H. Levitt: ORCID iD orcid.org/0000-0001-9878-1180

Catalogue record

Date deposited: 11 Oct 2017 16:31
Last modified: 10 Sep 2019 00:48

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Contributors

Author: Irene Marco-Rius
Author: Michael C. D. Tayler
Author: Mikko I. Kettunen
Author: Timothy J. Larkin
Author: Kerstin N. Timm
Author: Eva M. Serrao
Author: Tiago B. Rodrigues
Author: Giuseppe Pileio ORCID iD
Author: Jan Henrik Ardenkjaer-Larsen
Author: Kevin M. Brindle

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