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The preparation of jellyfish for stable isotope analysis

The preparation of jellyfish for stable isotope analysis
The preparation of jellyfish for stable isotope analysis
Gelatinous zooplankton are important predators, prey, and nutrient conduits within marine ecosystems. Information obtained from jellyfish stable isotope compositions can be invaluable to biological and environmental research
and management. Protocols for best practice in preparing jellyfish for stable isotope analysis, however, require standardisation to provide consistently comparable data, with ecologically significant changes in values due to freezing
reported in the literature. Jellyfish are easily sampled during standard marine fieldwork, and usually frozen before analysis. Here, mesoglea from freshly caught moon jelly, Aurelia aurita, were treated by thorough washing and/or freezing, and compared with untreated sections from the same individuals. Jellyfish were captured in July 2013 from Buckler’s Hard Marina in southern UK (latitude: 50.801, longitude: − 1.423). Isotope and element ratio changes of carbon and nitrogen composition due to the treatment of the mesoglea
were quantified. Both washing and freezing elevated δ15N values, with washing also decreasing the variance observed in these values. Untreated mesoglea showed the lowest δ13C values. Carbon-to-nitrogen elemental ratio increased with both washing and freezing. These results imply the presence of a water-soluble, isotopically depleted nitrogenous component in fresh jellyfish mesoglea. The concentration of this component varies among individuals, and thorough washing or freezing are recommended to ensure consistent stable isotope analyses of jellyfish mesoglea. This study describes a methodology aimed at improving the consistency and repeatability of stable isotope analyses of jellyfish.
Jellyfish, Stable isotope analysis
0025-3162
Mackenzie, Kirsteen M.
512f2b73-f8e4-4ab4-8d91-16c0a2084120
Trueman, Clive N.
d00d3bd6-a47b-4d47-89ae-841c3d506205
Lucas, Cathy H.
521743e3-b250-4c6b-b084-780af697d6bf
Bortoluzzi, Jenny
4f01e037-36bb-4b42-ad49-1693bf917ede
Mackenzie, Kirsteen M.
512f2b73-f8e4-4ab4-8d91-16c0a2084120
Trueman, Clive N.
d00d3bd6-a47b-4d47-89ae-841c3d506205
Lucas, Cathy H.
521743e3-b250-4c6b-b084-780af697d6bf
Bortoluzzi, Jenny
4f01e037-36bb-4b42-ad49-1693bf917ede

Mackenzie, Kirsteen M., Trueman, Clive N., Lucas, Cathy H. and Bortoluzzi, Jenny (2017) The preparation of jellyfish for stable isotope analysis. Marine Biology, 164, [219]. (doi:10.1007/s00227-017-3242-6).

Record type: Article

Abstract

Gelatinous zooplankton are important predators, prey, and nutrient conduits within marine ecosystems. Information obtained from jellyfish stable isotope compositions can be invaluable to biological and environmental research
and management. Protocols for best practice in preparing jellyfish for stable isotope analysis, however, require standardisation to provide consistently comparable data, with ecologically significant changes in values due to freezing
reported in the literature. Jellyfish are easily sampled during standard marine fieldwork, and usually frozen before analysis. Here, mesoglea from freshly caught moon jelly, Aurelia aurita, were treated by thorough washing and/or freezing, and compared with untreated sections from the same individuals. Jellyfish were captured in July 2013 from Buckler’s Hard Marina in southern UK (latitude: 50.801, longitude: − 1.423). Isotope and element ratio changes of carbon and nitrogen composition due to the treatment of the mesoglea
were quantified. Both washing and freezing elevated δ15N values, with washing also decreasing the variance observed in these values. Untreated mesoglea showed the lowest δ13C values. Carbon-to-nitrogen elemental ratio increased with both washing and freezing. These results imply the presence of a water-soluble, isotopically depleted nitrogenous component in fresh jellyfish mesoglea. The concentration of this component varies among individuals, and thorough washing or freezing are recommended to ensure consistent stable isotope analyses of jellyfish mesoglea. This study describes a methodology aimed at improving the consistency and repeatability of stable isotope analyses of jellyfish.

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Accepted/In Press date: 6 October 2017
e-pub ahead of print date: 30 October 2017
Published date: 1 December 2017
Keywords: Jellyfish, Stable isotope analysis

Identifiers

Local EPrints ID: 415294
URI: http://eprints.soton.ac.uk/id/eprint/415294
ISSN: 0025-3162
PURE UUID: 8ee738c9-482d-479b-8968-3997c346f5e9
ORCID for Cathy H. Lucas: ORCID iD orcid.org/0000-0002-5929-7481

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Date deposited: 07 Nov 2017 17:30
Last modified: 07 Oct 2020 01:37

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Contributors

Author: Kirsteen M. Mackenzie
Author: Cathy H. Lucas ORCID iD
Author: Jenny Bortoluzzi

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