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Cooperative interactions between NO and H2S: chemistry, biology, physiology, pathophysiology

Cooperative interactions between NO and H2S: chemistry, biology, physiology, pathophysiology
Cooperative interactions between NO and H2S: chemistry, biology, physiology, pathophysiology
The mutual interaction of sulfide and NO (also known as the “NO/H2S cross-talk”) has both chemical and biological foundations. With
respect to the chemical aspects, the formation of an intermediary nitrosothiol was proposed after the reaction of NO and H2S, which was
initially attributed to the highly reactive and short-lived HSNO. Subsequent chemical studies focusing on the reactivity of aqueous sulfide
solutions with nitrosothiols, peroxynitrite, and NO donors such as sodium nitroprusside revealed that the kinetics and reaction mechanisms
are complex and only partially understood. Recent work in this area resulted in the rediscovery of a forgotten chemistry of S-N hybrid
molecules, a class of compounds characterized more than half a century ago. Other lines of investigations revealed that the reaction of sulfide
with NO or nitrosothiols under biologically relevant conditions leads to formation of three different classes of intermediates including
ONSS− (perthionitrite, nitrosopersulfide), S − x
2 (polysulfides of different chain lengths), and ON(NO)SO−3 (the NONOate SULFI/NO);
their generation contributes to NO scavenging, NO storage and transport, redox conversion of NO to HNO, and sulfane sulfur signaling.
These reactions with sulfide are proposed to be of fundamental significance for NO signaling by modulating its bioactivity via conversion
to new chemical entities with properties distinct from those of NO. The release of NO and H2S from various stable or semistable chemical
“pools” can contribute to the biological responses to NO and H2S. With respect to the biological aspects, one level of interaction between
NO and H2S relates to their convergence on AKT PI3K signaling, in part due to the activation of PI3K by H2S and in part due to the inhibition
of phosphatase and tensin homolog (PTEN) by H2S. These processes lead to increased intracellular PIP3 levels, which, in turn, lead to
the activation of NO synthase due to a combination of phosphorylation and dephosphorylation of critical tyrosine residues. In addition, H2S
and NO signaling converge on various checkpoints of the guanylate cyclase/cGMP/protein kinase G; H2S can reduce guanylate cyclase,
thereby preventing its oxidative inactivation; H2S can inhibit phosphodiesterases, thereby prolonging the biological half-life of cGMP, and
H2S-derived polysulfides can activate protein kinase G. Moreover, H2S can directly interact with NOS, resulting in its sulfhydrative activation
and its stabilization in the physiological, dimeric state. In many experimental models, the protective or vascular effects of H2S are diminished
or are absent in the absence of functional eNOS, further underlining the critical importance of cooperative NO/H2S interactions.
57-83
Academic Press
Kevil, Christopher G.
1dc237a6-5468-46a9-bb66-c954cb1f005b
Cortese-Krott, Miriam M.
7dc9b44c-847c-4196-8866-a3cc0c1dc357
Nagy, Péter
ef870ecd-2ce8-494f-86da-7260d2a7c2a4
Papapetropoulos, Andreas
292296ed-ee5b-4a5e-93e0-8d38e164a7dd
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Szabo, Csaba
a7ffa0c5-92f8-4f50-9ace-b3618710ac4a
Freeman, Bruce
Kevil, Christopher G.
1dc237a6-5468-46a9-bb66-c954cb1f005b
Cortese-Krott, Miriam M.
7dc9b44c-847c-4196-8866-a3cc0c1dc357
Nagy, Péter
ef870ecd-2ce8-494f-86da-7260d2a7c2a4
Papapetropoulos, Andreas
292296ed-ee5b-4a5e-93e0-8d38e164a7dd
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Szabo, Csaba
a7ffa0c5-92f8-4f50-9ace-b3618710ac4a
Freeman, Bruce

Kevil, Christopher G., Cortese-Krott, Miriam M., Nagy, Péter, Papapetropoulos, Andreas, Feelisch, Martin and Szabo, Csaba (2017) Cooperative interactions between NO and H2S: chemistry, biology, physiology, pathophysiology. In, Freeman, Bruce (ed.) Nitric Oxide. 3rd ed. Academic Press, pp. 57-83. (doi:10.1016/B978-0-12-804273-1.00005-3).

Record type: Book Section

Abstract

The mutual interaction of sulfide and NO (also known as the “NO/H2S cross-talk”) has both chemical and biological foundations. With
respect to the chemical aspects, the formation of an intermediary nitrosothiol was proposed after the reaction of NO and H2S, which was
initially attributed to the highly reactive and short-lived HSNO. Subsequent chemical studies focusing on the reactivity of aqueous sulfide
solutions with nitrosothiols, peroxynitrite, and NO donors such as sodium nitroprusside revealed that the kinetics and reaction mechanisms
are complex and only partially understood. Recent work in this area resulted in the rediscovery of a forgotten chemistry of S-N hybrid
molecules, a class of compounds characterized more than half a century ago. Other lines of investigations revealed that the reaction of sulfide
with NO or nitrosothiols under biologically relevant conditions leads to formation of three different classes of intermediates including
ONSS− (perthionitrite, nitrosopersulfide), S − x
2 (polysulfides of different chain lengths), and ON(NO)SO−3 (the NONOate SULFI/NO);
their generation contributes to NO scavenging, NO storage and transport, redox conversion of NO to HNO, and sulfane sulfur signaling.
These reactions with sulfide are proposed to be of fundamental significance for NO signaling by modulating its bioactivity via conversion
to new chemical entities with properties distinct from those of NO. The release of NO and H2S from various stable or semistable chemical
“pools” can contribute to the biological responses to NO and H2S. With respect to the biological aspects, one level of interaction between
NO and H2S relates to their convergence on AKT PI3K signaling, in part due to the activation of PI3K by H2S and in part due to the inhibition
of phosphatase and tensin homolog (PTEN) by H2S. These processes lead to increased intracellular PIP3 levels, which, in turn, lead to
the activation of NO synthase due to a combination of phosphorylation and dephosphorylation of critical tyrosine residues. In addition, H2S
and NO signaling converge on various checkpoints of the guanylate cyclase/cGMP/protein kinase G; H2S can reduce guanylate cyclase,
thereby preventing its oxidative inactivation; H2S can inhibit phosphodiesterases, thereby prolonging the biological half-life of cGMP, and
H2S-derived polysulfides can activate protein kinase G. Moreover, H2S can directly interact with NOS, resulting in its sulfhydrative activation
and its stabilization in the physiological, dimeric state. In many experimental models, the protective or vascular effects of H2S are diminished
or are absent in the absence of functional eNOS, further underlining the critical importance of cooperative NO/H2S interactions.

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Published date: 1 July 2017

Identifiers

Local EPrints ID: 415320
URI: https://eprints.soton.ac.uk/id/eprint/415320
PURE UUID: f8d43a87-4562-4997-8d60-6c4856991a9f
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

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Date deposited: 07 Nov 2017 17:30
Last modified: 10 Dec 2019 01:37

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