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Characterization of the human, mouse and rat PGC1β (peroxisomeproliferator-activated receptor-γ co-activator 1β) gene in vitro and in vivo

Characterization of the human, mouse and rat PGC1β (peroxisomeproliferator-activated receptor-γ co-activator 1β) gene in vitro and in vivo
Characterization of the human, mouse and rat PGC1β (peroxisomeproliferator-activated receptor-γ co-activator 1β) gene in vitro and in vivo

PGC1α is a co-activator involved in adaptive thermogenesis, fatty-acid oxidation and gluconeogenesis. We describe the identification of several isoforms of a new human PGC1α homologue, cloned independently and named PGC1β. The human PGC1β gene is localized to chromosome 5, has 13 exons and spans more than 78 kb. Two different 5′ and 3′ ends due to differential splicing were identified by rapid amplification of cDNA ends PCR and screening of human cDNA libraries. We show that PGC1β variants in humans, mice and rats are expressed predominantly in heart, brown adipose tissue, brain and skeletal muscle. PGC1β expression, unlike PGC1α, is not up-regulated in brown adipose tissue in response to cold or obesity. Fasting experiments showed that PGC1α, but not PGC1β, is induced in liver and this suggests that only PGC1α is involved in the hepatic gluconeogenesis. No changes in PGC1β gene expression were observed associated with exercise. Human PGC1β-1a and -2a isoforms localized to the cell nucleus and, specifically, the isoform PGC1β-1a co-activated peroxisome-proliferator-activated receptor-γ, -α and the thyroid hormone receptor β1. Finally, we show that ectopic expression PGC1β leads to increased mitochondrial number and basal oxygen consumption. These results suggest that PGC1β may play a role in constitutive adrenergic-independent mitochondrial biogenesis.

Gene expression, Genomic structure, Isoform, Mitochondrial biogenesis, Peroxisome-proliferator-activated receptor-γ co-activator 1α (PGC1α), PGC1β
0264-6021
155-165
Meirhaeghe, Aline
57ef32ef-772e-4950-bdc9-41839ff8e2af
Crowley, Vivion
0b8c7961-5a1a-4ac5-b228-54a2340d586f
Lenaghan, Carol
02c19da5-b4ba-4ac9-b0cd-a296f0207208
Lelliott, Christopher
96092ff8-4f61-4fb7-87f4-e54b7216b78b
Green, Kath
94757198-7bf5-4e16-bd3e-ac05fe794460
Stewart, Abigail
6e164dfa-2f0b-4521-9e2c-a9da08085d40
Hart, Kevin
e03ab3e2-7803-467a-8ec2-70f71468b3c9
Schinner, Sven
43378156-7a07-41ec-8b88-96c501a589d2
Sethi, Jaswinder K.
923f1a81-91e4-46cd-8853-bb4a979f5a85
Yeo, Giles
b77367f2-8756-4278-8df8-178cac43ea88
Brand, Martin D.
9f5ac8dc-d2c4-41b3-bd5f-8298115c059d
Cortright, Ron N.
4ed3e2b2-2789-4111-bb30-3682e46830c7
O'Rahilly, Stephen
e5c7869f-10d1-4fdc-a564-e4907be415a3
Montague, Carl
887292be-08ec-45a3-9243-347ce1d55c40
Vidal-Puig, Antonio J.
bd6ec045-f9b8-48ff-ae86-a86a6a513435
Meirhaeghe, Aline
57ef32ef-772e-4950-bdc9-41839ff8e2af
Crowley, Vivion
0b8c7961-5a1a-4ac5-b228-54a2340d586f
Lenaghan, Carol
02c19da5-b4ba-4ac9-b0cd-a296f0207208
Lelliott, Christopher
96092ff8-4f61-4fb7-87f4-e54b7216b78b
Green, Kath
94757198-7bf5-4e16-bd3e-ac05fe794460
Stewart, Abigail
6e164dfa-2f0b-4521-9e2c-a9da08085d40
Hart, Kevin
e03ab3e2-7803-467a-8ec2-70f71468b3c9
Schinner, Sven
43378156-7a07-41ec-8b88-96c501a589d2
Sethi, Jaswinder K.
923f1a81-91e4-46cd-8853-bb4a979f5a85
Yeo, Giles
b77367f2-8756-4278-8df8-178cac43ea88
Brand, Martin D.
9f5ac8dc-d2c4-41b3-bd5f-8298115c059d
Cortright, Ron N.
4ed3e2b2-2789-4111-bb30-3682e46830c7
O'Rahilly, Stephen
e5c7869f-10d1-4fdc-a564-e4907be415a3
Montague, Carl
887292be-08ec-45a3-9243-347ce1d55c40
Vidal-Puig, Antonio J.
bd6ec045-f9b8-48ff-ae86-a86a6a513435

Meirhaeghe, Aline, Crowley, Vivion, Lenaghan, Carol, Lelliott, Christopher, Green, Kath, Stewart, Abigail, Hart, Kevin, Schinner, Sven, Sethi, Jaswinder K., Yeo, Giles, Brand, Martin D., Cortright, Ron N., O'Rahilly, Stephen, Montague, Carl and Vidal-Puig, Antonio J. (2003) Characterization of the human, mouse and rat PGC1β (peroxisomeproliferator-activated receptor-γ co-activator 1β) gene in vitro and in vivo. Biochemical Journal, 373 (1), 155-165. (doi:10.1042/BJ20030200).

Record type: Article

Abstract

PGC1α is a co-activator involved in adaptive thermogenesis, fatty-acid oxidation and gluconeogenesis. We describe the identification of several isoforms of a new human PGC1α homologue, cloned independently and named PGC1β. The human PGC1β gene is localized to chromosome 5, has 13 exons and spans more than 78 kb. Two different 5′ and 3′ ends due to differential splicing were identified by rapid amplification of cDNA ends PCR and screening of human cDNA libraries. We show that PGC1β variants in humans, mice and rats are expressed predominantly in heart, brown adipose tissue, brain and skeletal muscle. PGC1β expression, unlike PGC1α, is not up-regulated in brown adipose tissue in response to cold or obesity. Fasting experiments showed that PGC1α, but not PGC1β, is induced in liver and this suggests that only PGC1α is involved in the hepatic gluconeogenesis. No changes in PGC1β gene expression were observed associated with exercise. Human PGC1β-1a and -2a isoforms localized to the cell nucleus and, specifically, the isoform PGC1β-1a co-activated peroxisome-proliferator-activated receptor-γ, -α and the thyroid hormone receptor β1. Finally, we show that ectopic expression PGC1β leads to increased mitochondrial number and basal oxygen consumption. These results suggest that PGC1β may play a role in constitutive adrenergic-independent mitochondrial biogenesis.

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More information

Published date: 1 July 2003
Keywords: Gene expression, Genomic structure, Isoform, Mitochondrial biogenesis, Peroxisome-proliferator-activated receptor-γ co-activator 1α (PGC1α), PGC1β

Identifiers

Local EPrints ID: 415404
URI: http://eprints.soton.ac.uk/id/eprint/415404
ISSN: 0264-6021
PURE UUID: c42c3c39-7701-477d-9a3f-f95c45187988
ORCID for Jaswinder K. Sethi: ORCID iD orcid.org/0000-0003-4157-0475

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Date deposited: 09 Nov 2017 17:30
Last modified: 17 Dec 2019 01:27

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Contributors

Author: Aline Meirhaeghe
Author: Vivion Crowley
Author: Carol Lenaghan
Author: Christopher Lelliott
Author: Kath Green
Author: Abigail Stewart
Author: Kevin Hart
Author: Sven Schinner
Author: Giles Yeo
Author: Martin D. Brand
Author: Ron N. Cortright
Author: Stephen O'Rahilly
Author: Carl Montague
Author: Antonio J. Vidal-Puig

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