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It is complicated: curvature, diffusion and lipid sorting within the two membranes of Escherichia coli

It is complicated: curvature, diffusion and lipid sorting within the two membranes of Escherichia coli
It is complicated: curvature, diffusion and lipid sorting within the two membranes of Escherichia coli
The cell envelope of Gram-negative bacteria is composed of two membranes separated by a soluble region. Here, we report microsecond time scale coarse-grained molecular dynamics simulations of models of the Escherichia coli cell envelope that incorporate both membranes and various native membrane proteins. Our results predict that both the inner and outer membranes curve in a manner dependent on the size of the embedded proteins. The tightly cross-linked lipopolysaccharide molecules (LPS) of the outer membrane cause a strong coupling between the movement of proteins and lipids. While the flow of phospholipids is more random, their diffusion is nevertheless influenced by nearby proteins. Our results reveal protein-induced lipid sorting, whereby cardiolipin is significantly enriched within the vicinity of the water channel AqpZ and the multidrug efflux pump AcrBZ. In summary, our results provide unprecedented details of the intricate relationship between both membranes of E. coli and the proteins embedded within them.
1948-7185
5513-5518
Hsu, Pin-Chia
4b4c8c18-752e-4ba8-a456-0fc71df1b286
Samsudin, Firdaus
0f4f91f5-5c45-4041-897a-8943fafc214b
Shearer, Jonathan
44810f0c-f875-465e-be3b-810155814bc5
Khalid, Syma
90fbd954-7248-4f47-9525-4d6af9636394
Hsu, Pin-Chia
4b4c8c18-752e-4ba8-a456-0fc71df1b286
Samsudin, Firdaus
0f4f91f5-5c45-4041-897a-8943fafc214b
Shearer, Jonathan
44810f0c-f875-465e-be3b-810155814bc5
Khalid, Syma
90fbd954-7248-4f47-9525-4d6af9636394

Hsu, Pin-Chia, Samsudin, Firdaus, Shearer, Jonathan and Khalid, Syma (2017) It is complicated: curvature, diffusion and lipid sorting within the two membranes of Escherichia coli. The Journal of Physical Chemistry Letters, 8 (2), 5513-5518. (doi:10.1021/acs.jpclett.7b02432).

Record type: Article

Abstract

The cell envelope of Gram-negative bacteria is composed of two membranes separated by a soluble region. Here, we report microsecond time scale coarse-grained molecular dynamics simulations of models of the Escherichia coli cell envelope that incorporate both membranes and various native membrane proteins. Our results predict that both the inner and outer membranes curve in a manner dependent on the size of the embedded proteins. The tightly cross-linked lipopolysaccharide molecules (LPS) of the outer membrane cause a strong coupling between the movement of proteins and lipids. While the flow of phospholipids is more random, their diffusion is nevertheless influenced by nearby proteins. Our results reveal protein-induced lipid sorting, whereby cardiolipin is significantly enriched within the vicinity of the water channel AqpZ and the multidrug efflux pump AcrBZ. In summary, our results provide unprecedented details of the intricate relationship between both membranes of E. coli and the proteins embedded within them.

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Accepted/In Press date: 20 October 2017
e-pub ahead of print date: 31 October 2017
Published date: 16 November 2017

Identifiers

Local EPrints ID: 415574
URI: http://eprints.soton.ac.uk/id/eprint/415574
ISSN: 1948-7185
PURE UUID: 66a3bf77-e470-4bf1-a215-ef086692a05b
ORCID for Syma Khalid: ORCID iD orcid.org/0000-0002-3694-5044

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Date deposited: 15 Nov 2017 17:30
Last modified: 07 Oct 2020 05:36

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Contributors

Author: Pin-Chia Hsu
Author: Firdaus Samsudin
Author: Jonathan Shearer
Author: Syma Khalid ORCID iD

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