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Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress

Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress
Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress

Background:

P2- type calcium ATPases (ACAs-auto inhibited calcium ATPases and ECAs-endoplasmic reticulum calcium ATPases) belong to the P- type ATPase family of active membrane transporters and are significantly involved in maintaining accurate levels of Ca2+, Mn2+ and Zn2+ in the cytosol as well as playing a very important role in stress signaling, stomatal opening and closing and pollen tube growth. Here we report the identification and possible role of some of these ATPases from wheat.

Results:

In this study, ACA and ECA sequences of six species (belonging to Poaceae) were retrieved from different databases and a phylogenetic tree was constructed. A high degree of evolutionary relatedness was observed among P2 sequences characterized in this study. Members of the respective groups from different plant species were observed to fall under the same clade. This pattern highlights the common ancestry of P2− type calcium ATPases. Furthermore, qRT-PCR was used to analyse the expression of selected ACAs and ECAs from Triticum aestivum (wheat) under calcium toxicity and calcium deficiency. The data indicated that expression of ECAs is enhanced under calcium stress, suggesting possible roles of these ATPases in calcium homeostasis in wheat. Similarly, the expression of ACAs was significantly different in plants grown under calcium stress as compared to plants grown under control conditions. This gives clues to the role of ACAs in signal transduction during calcium stress in wheat.

Conclusion:

Here we concluded that wheat genome consists of nine P2B and three P2A -type calcium ATPases. Moreover, gene loss events in wheat ancestors lead to the loss of a particular homoeolog of a gene in wheat. To elaborate the role of these wheat ATPases, qRT-PCR was performed. The results indicated that when plants are exposed to calcium stress, both P2A and P2B gene expression get enhanced. This further gives clues about the possible role of these ATPases in wheat in calcium management. These findings can be useful in future for genetic manipulations as well as in wheat genome annotation process.
1471-2229
Aslam, Roohi
6ab1ca51-cf4c-44f5-844b-758d2c56cef8
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Bhatti, Muhammad Faraz
7a6fafa0-3330-4bc1-9bfe-19dd0cdc9f87
Virk, Nasar
65289231-fd60-4c20-8ae6-78164b4c5b29
Aslam, Roohi
6ab1ca51-cf4c-44f5-844b-758d2c56cef8
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Bhatti, Muhammad Faraz
7a6fafa0-3330-4bc1-9bfe-19dd0cdc9f87
Virk, Nasar
65289231-fd60-4c20-8ae6-78164b4c5b29

Aslam, Roohi, Williams, Lorraine E., Bhatti, Muhammad Faraz and Virk, Nasar (2017) Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress. BMC Plant Biology, 17 (174). (doi:10.1186/s12870-017-1112-5).

Record type: Article

Abstract


Background:

P2- type calcium ATPases (ACAs-auto inhibited calcium ATPases and ECAs-endoplasmic reticulum calcium ATPases) belong to the P- type ATPase family of active membrane transporters and are significantly involved in maintaining accurate levels of Ca2+, Mn2+ and Zn2+ in the cytosol as well as playing a very important role in stress signaling, stomatal opening and closing and pollen tube growth. Here we report the identification and possible role of some of these ATPases from wheat.

Results:

In this study, ACA and ECA sequences of six species (belonging to Poaceae) were retrieved from different databases and a phylogenetic tree was constructed. A high degree of evolutionary relatedness was observed among P2 sequences characterized in this study. Members of the respective groups from different plant species were observed to fall under the same clade. This pattern highlights the common ancestry of P2− type calcium ATPases. Furthermore, qRT-PCR was used to analyse the expression of selected ACAs and ECAs from Triticum aestivum (wheat) under calcium toxicity and calcium deficiency. The data indicated that expression of ECAs is enhanced under calcium stress, suggesting possible roles of these ATPases in calcium homeostasis in wheat. Similarly, the expression of ACAs was significantly different in plants grown under calcium stress as compared to plants grown under control conditions. This gives clues to the role of ACAs in signal transduction during calcium stress in wheat.

Conclusion:

Here we concluded that wheat genome consists of nine P2B and three P2A -type calcium ATPases. Moreover, gene loss events in wheat ancestors lead to the loss of a particular homoeolog of a gene in wheat. To elaborate the role of these wheat ATPases, qRT-PCR was performed. The results indicated that when plants are exposed to calcium stress, both P2A and P2B gene expression get enhanced. This further gives clues about the possible role of these ATPases in wheat in calcium management. These findings can be useful in future for genetic manipulations as well as in wheat genome annotation process.

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More information

Accepted/In Press date: 9 October 2017
e-pub ahead of print date: 27 October 2017

Identifiers

Local EPrints ID: 415662
URI: https://eprints.soton.ac.uk/id/eprint/415662
ISSN: 1471-2229
PURE UUID: b08c3ac0-3ce5-4431-a8c1-63e7521fc555

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Date deposited: 17 Nov 2017 17:30
Last modified: 13 Mar 2019 19:14

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