RANTES release by human adipose tissue in vivo and evidence for depot-specific differences
RANTES release by human adipose tissue in vivo and evidence for depot-specific differences
Obesity is associated with elevated inflammatory signals from various adipose tissue depots. This study aimed to evaluate release of regulated on activation, normal T cell expressed and secreted (RANTES) by human adipose tissue in vivo and ex vivo, in reference to monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) release. Arteriovenous differences of RANTES, MCP-1, and IL-6 were studied in vivo across the abdominal subcutaneous adipose tissue in healthy Caucasian subjects with a wide range of adiposity. Systemic levels and ex vivo RANTES release were studied in abdominal subcutaneous, gastric fat pad, and omental adipose tissue from morbidly obese bariatric surgery patients and in thoracic subcutaneous and epicardial adipose tissue from cardiac surgery patients without coronary artery disease. Arteriovenous studies confirmed in vivo RANTES and IL-6 release in adipose tissue of lean and obese subjects and release of MCP-1 in obesity. However, in vivo release of MCP-1 and RANTES, but not IL-6, was lower than circulating levels. Ex vivo release of RANTES was greater from the gastric fat pad compared with omental (P = 0.01) and subcutaneous (P = 0.001) tissue. Epicardial adipose tissue released less RANTES than thoracic subcutaneous adipose tissue in lean (P = 0.04) but not obese subjects. Indexes of obesity correlated with epicardial RANTES but not with systemic RANTES or its release from other depots. In conclusion, RANTES is released by human subcutaneous adipose tissue in vivo and in varying amounts by other depots ex vivo. While it appears unlikely that the adipose organ contributes significantly to circulating levels, local implications of this chemokine deserve further investigation.
E1262-E1268
Madani, Rana
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Karastergiou,, Kalypso
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Ogston, Nicola C.
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Miheisi, Nazar
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Bhome, Rahul
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Haloob, Nora
f84d68c6-7eda-4b38-ab35-fd43b666c340
Tan, Garry D.
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Karpe, Fredrik
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Malone-Lee, James
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Hashemi, Majid
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Jahangiri, Marjan
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Mohamed-Ali, Vidya
c5238dbb-3901-4fa4-9ed3-9300dd70fe84
25 May 2009
Madani, Rana
59d8f204-b703-4147-ad77-b5cfd75ddbf1
Karastergiou,, Kalypso
cf0861a1-146c-4d8f-a5db-f009819e2f73
Ogston, Nicola C.
5f17fae9-12ea-439a-b985-22df0b397070
Miheisi, Nazar
cba3def6-67ce-49e2-8fd1-6b89b3647557
Bhome, Rahul
d7b1e0d3-5925-460a-871d-5f52f69c649b
Haloob, Nora
f84d68c6-7eda-4b38-ab35-fd43b666c340
Tan, Garry D.
3445453f-0db2-4d43-a08a-25b36e8bdcef
Karpe, Fredrik
b804847f-a6b9-4b6a-9cdf-49a9ba84461d
Malone-Lee, James
46e0d42d-f286-41e0-9071-9bd37cf1c216
Hashemi, Majid
7891ad9e-15d4-4b41-9acb-4f50d2e45032
Jahangiri, Marjan
82874905-9a4a-4b7d-ae0e-0224678cf05d
Mohamed-Ali, Vidya
c5238dbb-3901-4fa4-9ed3-9300dd70fe84
Madani, Rana, Karastergiou,, Kalypso, Ogston, Nicola C., Miheisi, Nazar, Bhome, Rahul, Haloob, Nora, Tan, Garry D., Karpe, Fredrik, Malone-Lee, James, Hashemi, Majid, Jahangiri, Marjan and Mohamed-Ali, Vidya
(2009)
RANTES release by human adipose tissue in vivo and evidence for depot-specific differences.
American Journal of Physiology: Endocrinology and Metabolism, 296 (6), .
(doi:10.1152/ajpendo.90511.2008).
Abstract
Obesity is associated with elevated inflammatory signals from various adipose tissue depots. This study aimed to evaluate release of regulated on activation, normal T cell expressed and secreted (RANTES) by human adipose tissue in vivo and ex vivo, in reference to monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) release. Arteriovenous differences of RANTES, MCP-1, and IL-6 were studied in vivo across the abdominal subcutaneous adipose tissue in healthy Caucasian subjects with a wide range of adiposity. Systemic levels and ex vivo RANTES release were studied in abdominal subcutaneous, gastric fat pad, and omental adipose tissue from morbidly obese bariatric surgery patients and in thoracic subcutaneous and epicardial adipose tissue from cardiac surgery patients without coronary artery disease. Arteriovenous studies confirmed in vivo RANTES and IL-6 release in adipose tissue of lean and obese subjects and release of MCP-1 in obesity. However, in vivo release of MCP-1 and RANTES, but not IL-6, was lower than circulating levels. Ex vivo release of RANTES was greater from the gastric fat pad compared with omental (P = 0.01) and subcutaneous (P = 0.001) tissue. Epicardial adipose tissue released less RANTES than thoracic subcutaneous adipose tissue in lean (P = 0.04) but not obese subjects. Indexes of obesity correlated with epicardial RANTES but not with systemic RANTES or its release from other depots. In conclusion, RANTES is released by human subcutaneous adipose tissue in vivo and in varying amounts by other depots ex vivo. While it appears unlikely that the adipose organ contributes significantly to circulating levels, local implications of this chemokine deserve further investigation.
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Published date: 25 May 2009
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Local EPrints ID: 415704
URI: http://eprints.soton.ac.uk/id/eprint/415704
ISSN: 0193-1849
PURE UUID: 2fb5f850-22e0-40d5-b423-9c91c919d634
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Date deposited: 20 Nov 2017 17:30
Last modified: 16 Mar 2024 04:14
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Author:
Rana Madani
Author:
Kalypso Karastergiou,
Author:
Nicola C. Ogston
Author:
Nazar Miheisi
Author:
Nora Haloob
Author:
Garry D. Tan
Author:
Fredrik Karpe
Author:
James Malone-Lee
Author:
Majid Hashemi
Author:
Marjan Jahangiri
Author:
Vidya Mohamed-Ali
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