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Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane
Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane

We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

cell membrane, Fluorescence microscopy, laser-scanning, standing-wave excitation
SPIE
Amor, Rumelo
bd8b5ddf-7d00-44fc-880d-507e3293a588
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Amos, William Bradshaw
a2b96c03-3fec-48a3-8ee0-d41227897e77
McConnell, Gail
09172b64-80d3-47fd-ac78-de7b018873e0
Amor, Rumelo
bd8b5ddf-7d00-44fc-880d-507e3293a588
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Amos, William Bradshaw
a2b96c03-3fec-48a3-8ee0-d41227897e77
McConnell, Gail
09172b64-80d3-47fd-ac78-de7b018873e0

Amor, Rumelo, Mahajan, Sumeet, Amos, William Bradshaw and McConnell, Gail (2015) Standing-wave excitation of fluorescence in a laser-scanning microscope allows precise contour mapping of the red blood cell membrane. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII. vol. 9330, SPIE.. (doi:10.1117/12.2075644).

Record type: Conference or Workshop Item (Paper)

Abstract

We demonstrate fluorescence excitation at multiple planes in a laser-scanning microscope by using the standing wave from a mirror placed close to the specimen. We have observed precise modulation of the standing waves close to a mirror, with a frequency proportional to the Stokes shift, corresponding to a moiré pattern between the excitation and emission standing-wave fields. We use standing-wave excitation to plot the exact contour maps of the red blood cell membrane, with an axial resolution of ≈90 nm. The method may prove useful in the study of diseases which involve the surface membrane of red blood cells.

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More information

e-pub ahead of print date: 9 March 2015
Published date: 2015
Venue - Dates: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, San Francisco, United States, 2015-02-09 - 2015-02-12
Keywords: cell membrane, Fluorescence microscopy, laser-scanning, standing-wave excitation

Identifiers

Local EPrints ID: 415725
URI: https://eprints.soton.ac.uk/id/eprint/415725
PURE UUID: b536326a-72a9-4fc5-abd4-4a7b7a2c393c
ORCID for Sumeet Mahajan: ORCID iD orcid.org/0000-0001-8923-6666

Catalogue record

Date deposited: 21 Nov 2017 17:30
Last modified: 20 Jul 2019 00:52

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