Jonnalagadda, Umesh, Hill, Martyn, Messaoudi, Walid, Cook, Richard B., Oreffo, Richard O.C., Glynne-Jones, Peter and Tare, Rahul S. (2018) Acoustically modulated biomechanical stimulation for human cartilage tissue engineering. Lab on a Chip, 18 (3), 473-485. (doi:10.1039/c7lc01195d).
Abstract
Bioacoustofluidics can be used to trap and levitate cells within a fluid channel, thereby facilitating scaffold-free tissue engineering in a 3D environment. In the present study, we have designed and characterised an acoustofluidic bioreactor platform, which applies acoustic forces to mechanically stimulate aggregates of human articular chondrocytes in long-term levitated culture. By varying the acoustic parameters (amplitude, frequency sweep, and sweep repetition rate), cells were stimulated by oscillatory fluid shear stresses, which were dynamically modulated at different sweep repetition rates (1-50 Hz). Furthermore, in combination with appropriate biochemical cues, the acoustic stimulation was tuned to engineer human cartilage constructs with structural and mechanical properties comparable to those of native human cartilage, as assessed by immunohistology and nano-indentation, respectively. The findings of this study demonstrate the capability of acoustofluidics to provide a tuneable biomechanical force for the culture and development of hyaline-like human cartilage constructs in vitro.
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- Faculties (pre 2018 reorg) > Faculty of Medicine (pre 2018 reorg) > Human Development & Health (pre 2018 reorg)
Current Faculties > Faculty of Medicine > Human Development and Health > Human Development & Health (pre 2018 reorg)
Human Development and Health > Human Development & Health (pre 2018 reorg) - Faculties (pre 2011 reorg) > Faculty of Engineering Science & Maths (pre 2011 reorg) > Engineering Sciences (pre 2011 reorg) > Bioengineering Sciences (pre 2011 reorg)
- Faculties (pre 2018 reorg) > Faculty of Engineering and the Environment (pre 2018 reorg) > Mechanical Engineering (pre 2018 reorg) > nCATS Group (pre 2018 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > School of Engineering > Mechanical Engineering > Mechanical Engineering (pre 2018 reorg) > nCATS Group (pre 2018 reorg)
Mechanical Engineering > Mechanical Engineering (pre 2018 reorg) > nCATS Group (pre 2018 reorg) - Faculties (pre 2018 reorg) > Faculty of Engineering and the Environment (pre 2018 reorg) > Southampton Marine & Maritime Institute (pre 2018 reorg)
- Faculties (pre 2018 reorg) > Faculty of Natural and Environmental Sciences (pre 2018 reorg) > Institute for Life Sciences (pre 2018 reorg)
Current Faculties > Faculty of Environmental and Life Sciences > Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg)
Institute for Life Sciences > Institute for Life Sciences (pre 2018 reorg) - Faculties (pre 2018 reorg) > Faculty of Engineering and the Environment (pre 2018 reorg) > Mechanical Engineering (pre 2018 reorg) > Mechatronics (pre 2018 reorg)
Current Faculties > Faculty of Engineering and Physical Sciences > School of Engineering > Mechanical Engineering > Mechanical Engineering (pre 2018 reorg) > Mechatronics (pre 2018 reorg)
Mechanical Engineering > Mechanical Engineering (pre 2018 reorg) > Mechatronics (pre 2018 reorg) - Faculties (pre 2018 reorg) > Faculty of Engineering and the Environment (pre 2018 reorg) > Education Hub (pre 2018 reorg)
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