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Acoustically modulated biomechanical stimulation for human cartilage tissue engineering

Acoustically modulated biomechanical stimulation for human cartilage tissue engineering
Acoustically modulated biomechanical stimulation for human cartilage tissue engineering
Bioacoustofluidics can be used to trap and levitate cells within a fluid channel, thereby facilitating scaffold-free tissue engineering in a 3D environment. In the present study, we have designed and characterised an acoustofluidic bioreactor platform, which applies acoustic forces to mechanically stimulate aggregates of human articular chondrocytes in long-term levitated culture. By varying the acoustic parameters (amplitude, frequency sweep, and sweep repetition rate), cells were stimulated by oscillatory fluid shear stresses, which were dynamically modulated at different sweep repetition rates (1-50 Hz). Furthermore, in combination with appropriate biochemical cues, the acoustic stimulation was tuned to engineer human cartilage constructs with structural and mechanical properties comparable to those of native human cartilage, as assessed by immunohistology and nano-indentation, respectively. The findings of this study demonstrate the capability of acoustofluidics to provide a tuneable biomechanical force for the culture and development of hyaline-like human cartilage constructs in vitro.
1473-0197
473-485
Jonnalagadda, Umesh
8481bb68-d50f-4274-8e0f-4c1dc02bb3d7
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Messaoudi, Walid
d66ce719-d255-4197-b11c-39886853669a
Cook, Richard B.
06f8322d-81be-4f82-9326-19e55541c78f
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Jonnalagadda, Umesh
8481bb68-d50f-4274-8e0f-4c1dc02bb3d7
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Messaoudi, Walid
d66ce719-d255-4197-b11c-39886853669a
Cook, Richard B.
06f8322d-81be-4f82-9326-19e55541c78f
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a

Jonnalagadda, Umesh, Hill, Martyn, Messaoudi, Walid, Cook, Richard B., Oreffo, Richard O.C., Glynne-Jones, Peter and Tare, Rahul S. (2018) Acoustically modulated biomechanical stimulation for human cartilage tissue engineering. Lab on a Chip, 18 (3), 473-485. (doi:10.1039/c7lc01195d).

Record type: Article

Abstract

Bioacoustofluidics can be used to trap and levitate cells within a fluid channel, thereby facilitating scaffold-free tissue engineering in a 3D environment. In the present study, we have designed and characterised an acoustofluidic bioreactor platform, which applies acoustic forces to mechanically stimulate aggregates of human articular chondrocytes in long-term levitated culture. By varying the acoustic parameters (amplitude, frequency sweep, and sweep repetition rate), cells were stimulated by oscillatory fluid shear stresses, which were dynamically modulated at different sweep repetition rates (1-50 Hz). Furthermore, in combination with appropriate biochemical cues, the acoustic stimulation was tuned to engineer human cartilage constructs with structural and mechanical properties comparable to those of native human cartilage, as assessed by immunohistology and nano-indentation, respectively. The findings of this study demonstrate the capability of acoustofluidics to provide a tuneable biomechanical force for the culture and development of hyaline-like human cartilage constructs in vitro.

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Accepted/In Press date: 20 November 2017
e-pub ahead of print date: 4 January 2018
Published date: 30 January 2018

Identifiers

Local EPrints ID: 415972
URI: http://eprints.soton.ac.uk/id/eprint/415972
ISSN: 1473-0197
PURE UUID: 1d856c74-675f-490d-9974-aa44fffd8a9c
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448
ORCID for Richard O.C. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for Rahul S. Tare: ORCID iD orcid.org/0000-0001-8274-8837

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Date deposited: 29 Nov 2017 17:30
Last modified: 18 Feb 2021 17:02

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Contributors

Author: Martyn Hill ORCID iD
Author: Walid Messaoudi
Author: Richard B. Cook
Author: Rahul S. Tare ORCID iD

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