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Soft tissue 3D imaging in the lab through optimised propagation-based phase contrast computed tomography

Soft tissue 3D imaging in the lab through optimised propagation-based phase contrast computed tomography
Soft tissue 3D imaging in the lab through optimised propagation-based phase contrast computed tomography
Tomographic phase contrast imaging using hard X-rays is instrumental in revealing and understanding the three-dimensional (3D) anatomic structure of biological tissues. However, phase contrast imaging is often limited to synchrotron radiation sources to which access is limited and highly competitive. Therefore, it is critical to enable high-quality phase contrast tomography using lab-based X-ray sources. We present a lab-based soft tissue 3D imaging approach through optimised in-line phase contrast computed tomography, building upon and going beyond previous work of Bidola et al. [Opt Express 23(23), 30000-30013 (2015)]. Murine soleus muscle was used as a test specimen to systemically optimise source-to-sample and sample-to-detector distances, exposure time and the critical ratio used for Paganin phase retrieval before tomographic reconstruction. Larger propagation distances combined with longer exposure times resulted in improved image quality. Whilst the contrast-to-noise ratio of lab-based phase contrast imaging was found to be lower than that of synchrotron-based imaging, important microscopic soft tissue features, such as nerves, could well be distinguished in 3D from surrounding tissue for both imaging modalities. This shows that lab-based X-ray sources present a viable alternative to synchrotron radiation sources for tomographic phase contrast imaging of soft tissues.
soft tissue, 3D imaging, propagation-based, phase contrast, computed tomography
1094-4087
Zeller-Plumhoff, Berit
1a9d6525-3f1f-4a0b-89ba-e5042ceb37f4
Mead, Joshua L.
c4c41462-161a-4130-bd41-c4cdedd876a2
Tan, Deck
dc6dad3d-077f-4215-870b-0d75043ae66c
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Clough, Geraldine
9f19639e-a929-4976-ac35-259f9011c494
Boardman, Richard
5818d677-5732-4e8a-a342-7164dbb10df1
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad
Zeller-Plumhoff, Berit
1a9d6525-3f1f-4a0b-89ba-e5042ceb37f4
Mead, Joshua L.
c4c41462-161a-4130-bd41-c4cdedd876a2
Tan, Deck
dc6dad3d-077f-4215-870b-0d75043ae66c
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Clough, Geraldine
9f19639e-a929-4976-ac35-259f9011c494
Boardman, Richard
5818d677-5732-4e8a-a342-7164dbb10df1
Schneider, Philipp
a810f925-4808-44e4-8a4a-a51586f9d7ad

Zeller-Plumhoff, Berit, Mead, Joshua L., Tan, Deck, Roose, Tiina, Clough, Geraldine, Boardman, Richard and Schneider, Philipp (2017) Soft tissue 3D imaging in the lab through optimised propagation-based phase contrast computed tomography. Optics Express. (doi:10.1364/OE.25.033451).

Record type: Article

Abstract

Tomographic phase contrast imaging using hard X-rays is instrumental in revealing and understanding the three-dimensional (3D) anatomic structure of biological tissues. However, phase contrast imaging is often limited to synchrotron radiation sources to which access is limited and highly competitive. Therefore, it is critical to enable high-quality phase contrast tomography using lab-based X-ray sources. We present a lab-based soft tissue 3D imaging approach through optimised in-line phase contrast computed tomography, building upon and going beyond previous work of Bidola et al. [Opt Express 23(23), 30000-30013 (2015)]. Murine soleus muscle was used as a test specimen to systemically optimise source-to-sample and sample-to-detector distances, exposure time and the critical ratio used for Paganin phase retrieval before tomographic reconstruction. Larger propagation distances combined with longer exposure times resulted in improved image quality. Whilst the contrast-to-noise ratio of lab-based phase contrast imaging was found to be lower than that of synchrotron-based imaging, important microscopic soft tissue features, such as nerves, could well be distinguished in 3D from surrounding tissue for both imaging modalities. This shows that lab-based X-ray sources present a viable alternative to synchrotron radiation sources for tomographic phase contrast imaging of soft tissues.

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More information

Accepted/In Press date: 27 November 2017
e-pub ahead of print date: 22 December 2017
Keywords: soft tissue, 3D imaging, propagation-based, phase contrast, computed tomography

Identifiers

Local EPrints ID: 416104
URI: http://eprints.soton.ac.uk/id/eprint/416104
ISSN: 1094-4087
PURE UUID: 6e34b2e4-f749-4f9e-af4f-7e186385d777
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063
ORCID for Geraldine Clough: ORCID iD orcid.org/0000-0002-6226-8964
ORCID for Philipp Schneider: ORCID iD orcid.org/0000-0001-7499-3576

Catalogue record

Date deposited: 04 Dec 2017 17:30
Last modified: 07 Oct 2020 05:55

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