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Neural toxicity of retroviruses

Neural toxicity of retroviruses
Neural toxicity of retroviruses

Recombinant retroviruses containing the cDNA for human tyrosine hydroxylase-1 and Escherichia coli lacZ gene were used to infect primary foetal ventral mesencephalon and cortical cultures from rat brain. Severe neuronal toxicity resulted 3-4 days after infection, glial cells seemed to be much more resistant. The toxicity was likely to have resulted from an agent present within the virus-containing medium itself, rather than from the retrovirus itself. The results of this study indicate that retroviruses are not suitable vectors for the introduction of tyrosine hydroxylase into primary neuronal cultures.

Animals, Cerebral Cortex, Gene Expression, Gene Transfer Techniques, Genetic Vectors, Hexadimethrine Bromide, Lac Operon, Mesencephalon, Rats, Retroviridae, Tyrosine 3-Monooxygenase, Journal Article, Research Support, Non-U.S. Gov't
0304-3940
66-8
Hurley, Michael J.
07b0180f-8e32-4ce1-8185-e046a42ef15a
Bradford, Henry F.
38633187-8fea-4047-b82e-5eb4a8011ab7
O'Hare, Kevin
e8423fa8-9716-48bd-afb2-5e21f00beda0
Stern, Gerald M.
62ffc9ad-a2cf-4133-bc8d-2cedace905e8
Uchida, Kohichi
94591838-45bd-426b-91da-f9d60a055c6c
Hurley, Michael J.
07b0180f-8e32-4ce1-8185-e046a42ef15a
Bradford, Henry F.
38633187-8fea-4047-b82e-5eb4a8011ab7
O'Hare, Kevin
e8423fa8-9716-48bd-afb2-5e21f00beda0
Stern, Gerald M.
62ffc9ad-a2cf-4133-bc8d-2cedace905e8
Uchida, Kohichi
94591838-45bd-426b-91da-f9d60a055c6c

Hurley, Michael J., Bradford, Henry F., O'Hare, Kevin, Stern, Gerald M. and Uchida, Kohichi (1996) Neural toxicity of retroviruses. Neuroscience Letters, 220 (1), 66-8. (doi:10.1016/S0304-3940(96)13224-9).

Record type: Article

Abstract

Recombinant retroviruses containing the cDNA for human tyrosine hydroxylase-1 and Escherichia coli lacZ gene were used to infect primary foetal ventral mesencephalon and cortical cultures from rat brain. Severe neuronal toxicity resulted 3-4 days after infection, glial cells seemed to be much more resistant. The toxicity was likely to have resulted from an agent present within the virus-containing medium itself, rather than from the retrovirus itself. The results of this study indicate that retroviruses are not suitable vectors for the introduction of tyrosine hydroxylase into primary neuronal cultures.

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More information

Published date: 6 December 1996
Keywords: Animals, Cerebral Cortex, Gene Expression, Gene Transfer Techniques, Genetic Vectors, Hexadimethrine Bromide, Lac Operon, Mesencephalon, Rats, Retroviridae, Tyrosine 3-Monooxygenase, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416137
URI: https://eprints.soton.ac.uk/id/eprint/416137
ISSN: 0304-3940
PURE UUID: 1b554172-43d4-49fd-8fcd-4c25f238a28b

Catalogue record

Date deposited: 06 Dec 2017 17:30
Last modified: 13 Mar 2019 19:08

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