Dopamine D1 receptor expression in human basal ganglia and changes in Parkinson's disease
Dopamine D1 receptor expression in human basal ganglia and changes in Parkinson's disease
The expression of the human dopamine D1 receptor was examined by reverse transcription polymerase chain reaction (RT-PCR) and radioligand binding using [3H]-SCH23390 in post-mortem brain tissue that was obtained from normal subjects and patients dying with Parkinson's disease who were receiving treatment with dopaminergic drugs. D1 receptor mRNA and specific [3H]-SCH23390 binding sites were found in both striatal (nucleus accumbens, caudate nucleus and putamen) and extrastriatal (globus pallidus and substantia nigra) brain regions. In parkinsonian brain, D1 receptor mRNA was increased in the nucleus accumbens, while a decrease was detected in the substantia nigra pars compacta. No change in D1 mRNA levels was found in the other brain areas examined. An increase in the density of specific [3H]-SCH23390 binding sites was found in the anterior putamen and a decrease in the external segment of the globus pallidus, no changes were detected elsewhere. This study demonstrates that regulation of D1 receptor expression in the brain of patients dying with Parkinson's disease that were treated with L-DOPA is confined to small alterations in restricted brain regions.
Aged, Aged, 80 and over, Basal Ganglia, Benzazepines, Brain Chemistry, Dopamine Antagonists, Female, Gene Expression, Humans, Male, Middle Aged, Parkinson Disease, RNA, Messenger, Radioligand Assay, Receptors, Dopamine D1, Reverse Transcriptase Polymerase Chain Reaction, Tritium, Journal Article, Research Support, Non-U.S. Gov't
271-279
Hurley, Michael J.
07b0180f-8e32-4ce1-8185-e046a42ef15a
Mash, Deborah C.
8306c19c-264c-4b75-b534-39a039208779
Jenner, Peter
785c25df-ec64-4181-b057-26dd31cd9d84
5 March 2001
Hurley, Michael J.
07b0180f-8e32-4ce1-8185-e046a42ef15a
Mash, Deborah C.
8306c19c-264c-4b75-b534-39a039208779
Jenner, Peter
785c25df-ec64-4181-b057-26dd31cd9d84
Hurley, Michael J., Mash, Deborah C. and Jenner, Peter
(2001)
Dopamine D1 receptor expression in human basal ganglia and changes in Parkinson's disease.
Molecular Brain Research, 87 (2), .
(doi:10.1016/S0169-328X(01)00022-5).
Abstract
The expression of the human dopamine D1 receptor was examined by reverse transcription polymerase chain reaction (RT-PCR) and radioligand binding using [3H]-SCH23390 in post-mortem brain tissue that was obtained from normal subjects and patients dying with Parkinson's disease who were receiving treatment with dopaminergic drugs. D1 receptor mRNA and specific [3H]-SCH23390 binding sites were found in both striatal (nucleus accumbens, caudate nucleus and putamen) and extrastriatal (globus pallidus and substantia nigra) brain regions. In parkinsonian brain, D1 receptor mRNA was increased in the nucleus accumbens, while a decrease was detected in the substantia nigra pars compacta. No change in D1 mRNA levels was found in the other brain areas examined. An increase in the density of specific [3H]-SCH23390 binding sites was found in the anterior putamen and a decrease in the external segment of the globus pallidus, no changes were detected elsewhere. This study demonstrates that regulation of D1 receptor expression in the brain of patients dying with Parkinson's disease that were treated with L-DOPA is confined to small alterations in restricted brain regions.
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Published date: 5 March 2001
Keywords:
Aged, Aged, 80 and over, Basal Ganglia, Benzazepines, Brain Chemistry, Dopamine Antagonists, Female, Gene Expression, Humans, Male, Middle Aged, Parkinson Disease, RNA, Messenger, Radioligand Assay, Receptors, Dopamine D1, Reverse Transcriptase Polymerase Chain Reaction, Tritium, Journal Article, Research Support, Non-U.S. Gov't
Identifiers
Local EPrints ID: 416143
URI: http://eprints.soton.ac.uk/id/eprint/416143
ISSN: 0169-328X
PURE UUID: b910ffa8-87df-4dcb-96fe-d8b45efa98b6
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Date deposited: 06 Dec 2017 17:30
Last modified: 15 Mar 2024 17:13
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Author:
Michael J. Hurley
Author:
Deborah C. Mash
Author:
Peter Jenner
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