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Neisseria lactamica Y92-1009 complete genome sequence

Neisseria lactamica Y92-1009 complete genome sequence
Neisseria lactamica Y92-1009 complete genome sequence

We present the high quality, complete genome assembly of Neisseria lactamica Y92-1009 used to manufacture an outer membrane vesicle (OMV)-based vaccine, and a member of the Neisseria genus. The strain is available on request from the Public Health England Meningococcal Reference Unit. This Gram negative, dipplococcoid bacterium is an organism of worldwide clinical interest because human nasopharyngeal carriage is related inversely to the incidence of meningococcal disease, caused by Neisseria meningitidis. The organism sequenced was isolated during a school carriage survey in Northern Ireland in 1992 and has been the subject of a variety of laboratory and clinical studies. Four SMRT cells on a RSII machine by Pacific Biosystems were used to produce a complete, closed genome assembly. Sequence data were obtained for a total of 30,180,391 bases from 2621 reads and assembled using the HGAP algorithm. The assembly was corrected using short reads obtained from an Illumina HiSeq 2000instrument. This resulted in a 2,146,723 bp assembly with approximately 460 fold mean coverage depth and a GC ratio of 52.3%.

Journal Article
1944-3277
Pandey, Anish K.
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Cleary, David W.
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Laver, Jay R.
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Maiden, Martin C.J.
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Didelot, Xavier
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Gorringe, Andrew
503cb828-4e75-44e9-a8af-61f814e02bc6
Read, Robert C.
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Pandey, Anish K.
0be0525d-d74b-46ab-961d-611fd0dd759e
Cleary, David W.
f4079c6d-d54b-4108-b346-b0069035bec0
Laver, Jay R.
b2996398-2ccf-40f0-92b8-f338f3de796b
Maiden, Martin C.J.
721886a4-7548-45c8-882f-7a9cae695c0c
Didelot, Xavier
2d6849fa-f7a4-421d-a328-bacfc713af37
Gorringe, Andrew
503cb828-4e75-44e9-a8af-61f814e02bc6
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51

Pandey, Anish K., Cleary, David W., Laver, Jay R., Maiden, Martin C.J., Didelot, Xavier, Gorringe, Andrew and Read, Robert C. (2017) Neisseria lactamica Y92-1009 complete genome sequence. Standards in Genomic Sciences, 12 (41). (doi:10.1186/s40793-017-0250-6).

Record type: Article

Abstract

We present the high quality, complete genome assembly of Neisseria lactamica Y92-1009 used to manufacture an outer membrane vesicle (OMV)-based vaccine, and a member of the Neisseria genus. The strain is available on request from the Public Health England Meningococcal Reference Unit. This Gram negative, dipplococcoid bacterium is an organism of worldwide clinical interest because human nasopharyngeal carriage is related inversely to the incidence of meningococcal disease, caused by Neisseria meningitidis. The organism sequenced was isolated during a school carriage survey in Northern Ireland in 1992 and has been the subject of a variety of laboratory and clinical studies. Four SMRT cells on a RSII machine by Pacific Biosystems were used to produce a complete, closed genome assembly. Sequence data were obtained for a total of 30,180,391 bases from 2621 reads and assembled using the HGAP algorithm. The assembly was corrected using short reads obtained from an Illumina HiSeq 2000instrument. This resulted in a 2,146,723 bp assembly with approximately 460 fold mean coverage depth and a GC ratio of 52.3%.

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Accepted/In Press date: 7 July 2017
e-pub ahead of print date: 24 July 2017
Published date: 24 July 2017
Keywords: Journal Article

Identifiers

Local EPrints ID: 416151
URI: https://eprints.soton.ac.uk/id/eprint/416151
ISSN: 1944-3277
PURE UUID: 571780a0-0099-4a14-8ea9-852cde879168
ORCID for David W. Cleary: ORCID iD orcid.org/0000-0003-4533-0700
ORCID for Robert C. Read: ORCID iD orcid.org/0000-0002-4297-6728

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Date deposited: 06 Dec 2017 17:30
Last modified: 10 Dec 2019 01:37

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Contributors

Author: Anish K. Pandey
Author: David W. Cleary ORCID iD
Author: Jay R. Laver
Author: Martin C.J. Maiden
Author: Xavier Didelot
Author: Andrew Gorringe
Author: Robert C. Read ORCID iD

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