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Conspicuous morphological differentiation without speciation in Anemonia viridis (Cnidaria, Actiniaria)

Conspicuous morphological differentiation without speciation in Anemonia viridis (Cnidaria, Actiniaria)
Conspicuous morphological differentiation without speciation in Anemonia viridis (Cnidaria, Actiniaria)
Anemonia viridis is a model species for studies of physiological and transcriptomic response to symbiosis and environmental stress (temperature, light, symbiosis breakdown). Five morphs are described in this species, based on morphology, pigment protein content, and major mode of reproduction. Up to now, the taxonomic status of these morphs remains unclear, without clear knowledge of whether the morphological variation amongst the morphs is due to phenotypic plasticity or adaptation. In the present study, we assess the species status of the three most commonly found morphs by coalescent analyses. For this purpose, five markers were designed for genes whose expression is modified under stress (ca2m, duf140, RNAbinding5, tyrK, sym32) and analysed in 34 individuals representing the morphs A. viridis var rufescens, rustica and smaragdina from eight geographic sites (two in the English Channel and six in the Mediterranean Sea). Phylogenetic analyses of individual gene trees showed no clear separation of the morphs. Furthermore, multilocus coalescent analyses using SpedeSTEM and BPP regrouped all the morphs into one species, showing very few genetic differences amongst them. In a further analysis, we checked for clonality amongst 80 individuals of A. viridis var. smaragdina from one geographic site using three microsatellite loci. This morph proved to be as clonal as var. rustica, indicating a similar potential for asexual reproduction.
1477-2000
1-16
Mallien, Cédric
743d5d2d-8a30-4c0e-85da-22ec350efe39
Porro, Barbara
045b4e55-522f-4c33-a9b1-ca852931a5c6
Zamoum, Thamilla
68e4de31-9ffa-47d6-9c0b-54812cb7bf7b
Olivier, Caroline
b8dd6d91-ee6b-4ae8-a826-63f16d4ecac0
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Furla, Paola
d3e7b181-371a-4a7a-8749-594e704d10d7
Forcioli, Didier
20a5b8db-f55e-4a85-a1d4-e174250e73d8
Mallien, Cédric
743d5d2d-8a30-4c0e-85da-22ec350efe39
Porro, Barbara
045b4e55-522f-4c33-a9b1-ca852931a5c6
Zamoum, Thamilla
68e4de31-9ffa-47d6-9c0b-54812cb7bf7b
Olivier, Caroline
b8dd6d91-ee6b-4ae8-a826-63f16d4ecac0
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Furla, Paola
d3e7b181-371a-4a7a-8749-594e704d10d7
Forcioli, Didier
20a5b8db-f55e-4a85-a1d4-e174250e73d8

Mallien, Cédric, Porro, Barbara, Zamoum, Thamilla, Olivier, Caroline, Wiedenmann, Jörg, Furla, Paola and Forcioli, Didier (2017) Conspicuous morphological differentiation without speciation in Anemonia viridis (Cnidaria, Actiniaria). Systematics and Biodiversity, 1-16. (doi:10.1080/14772000.2017.1383948).

Record type: Article

Abstract

Anemonia viridis is a model species for studies of physiological and transcriptomic response to symbiosis and environmental stress (temperature, light, symbiosis breakdown). Five morphs are described in this species, based on morphology, pigment protein content, and major mode of reproduction. Up to now, the taxonomic status of these morphs remains unclear, without clear knowledge of whether the morphological variation amongst the morphs is due to phenotypic plasticity or adaptation. In the present study, we assess the species status of the three most commonly found morphs by coalescent analyses. For this purpose, five markers were designed for genes whose expression is modified under stress (ca2m, duf140, RNAbinding5, tyrK, sym32) and analysed in 34 individuals representing the morphs A. viridis var rufescens, rustica and smaragdina from eight geographic sites (two in the English Channel and six in the Mediterranean Sea). Phylogenetic analyses of individual gene trees showed no clear separation of the morphs. Furthermore, multilocus coalescent analyses using SpedeSTEM and BPP regrouped all the morphs into one species, showing very few genetic differences amongst them. In a further analysis, we checked for clonality amongst 80 individuals of A. viridis var. smaragdina from one geographic site using three microsatellite loci. This morph proved to be as clonal as var. rustica, indicating a similar potential for asexual reproduction.

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More information

Accepted/In Press date: 5 September 2017
e-pub ahead of print date: 13 November 2017

Identifiers

Local EPrints ID: 416191
URI: http://eprints.soton.ac.uk/id/eprint/416191
ISSN: 1477-2000
PURE UUID: 3da6d937-5717-48ff-8dd8-bc51c91b836a
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

Catalogue record

Date deposited: 07 Dec 2017 17:30
Last modified: 10 Nov 2021 03:18

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Contributors

Author: Cédric Mallien
Author: Barbara Porro
Author: Thamilla Zamoum
Author: Caroline Olivier
Author: Paola Furla
Author: Didier Forcioli

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