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Proteomic analysis of striatum from MPTP-treated marmosets (Callithrix jacchus) with L-DOPA-induced dyskinesia of differing severity

Proteomic analysis of striatum from MPTP-treated marmosets (Callithrix jacchus) with L-DOPA-induced dyskinesia of differing severity
Proteomic analysis of striatum from MPTP-treated marmosets (Callithrix jacchus) with L-DOPA-induced dyskinesia of differing severity
Marmosets rendered parkinsonian with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and treated with L-3,4-dihydroxyphenylalanine (L-DOPA) develop dyskinesia, but with differing degrees of severity. To provide insight into the molecular mechanisms responsible for the different level of dyskinesia to manifest in individual animals, proteins in striatum from MPTP-treated marmosets with different levels of L-DOPA-induced dyskinesia were separated by 2-dimensional (2-D) protein electrophoresis. Thirty-five differentially expressed proteins were identified by mass spectrometry and peptide mass fingerprinting, and comparative analysis found 10 were significantly increased and 3 had significantly reduced expression in animals with a high level of dyskinesia when compared to animals with a low incidence of dyskinesia. These proteins belonged to a range of functional classes, for example, molecular chaperones, metabolic enzymes and synaptic structural proteins. The findings of this study provide clues about the molecular mechanisms that cause dyskinesia to manifest and point towards potential novel targets for the development of therapeutic agents to prevent or treat established dyskinesia.
Animals, Callithrix, Dyskinesias, Levodopa, MPTP Poisoning, Neostriatum, Proteome, Journal Article
0895-8696
302-312
Hurley, Michael J.
07b0180f-8e32-4ce1-8185-e046a42ef15a
Jackson, Michael J.
e0200cfd-dc38-4060-b116-ee1d2d31fb8e
Smith, Lance A.
64767b7b-c1d1-45ca-8faa-658ccb84d3c7
Rose, Sarah
8e68d2a9-4f11-49cf-84dd-58e9badfe2fe
Jenner, Peter
785c25df-ec64-4181-b057-26dd31cd9d84
Hurley, Michael J.
07b0180f-8e32-4ce1-8185-e046a42ef15a
Jackson, Michael J.
e0200cfd-dc38-4060-b116-ee1d2d31fb8e
Smith, Lance A.
64767b7b-c1d1-45ca-8faa-658ccb84d3c7
Rose, Sarah
8e68d2a9-4f11-49cf-84dd-58e9badfe2fe
Jenner, Peter
785c25df-ec64-4181-b057-26dd31cd9d84

Hurley, Michael J., Jackson, Michael J., Smith, Lance A., Rose, Sarah and Jenner, Peter (2014) Proteomic analysis of striatum from MPTP-treated marmosets (Callithrix jacchus) with L-DOPA-induced dyskinesia of differing severity. Journal of Molecular Neuroscience, 52 (2), 302-312. (doi:10.1007/s12031-013-0145-y).

Record type: Article

Abstract

Marmosets rendered parkinsonian with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and treated with L-3,4-dihydroxyphenylalanine (L-DOPA) develop dyskinesia, but with differing degrees of severity. To provide insight into the molecular mechanisms responsible for the different level of dyskinesia to manifest in individual animals, proteins in striatum from MPTP-treated marmosets with different levels of L-DOPA-induced dyskinesia were separated by 2-dimensional (2-D) protein electrophoresis. Thirty-five differentially expressed proteins were identified by mass spectrometry and peptide mass fingerprinting, and comparative analysis found 10 were significantly increased and 3 had significantly reduced expression in animals with a high level of dyskinesia when compared to animals with a low incidence of dyskinesia. These proteins belonged to a range of functional classes, for example, molecular chaperones, metabolic enzymes and synaptic structural proteins. The findings of this study provide clues about the molecular mechanisms that cause dyskinesia to manifest and point towards potential novel targets for the development of therapeutic agents to prevent or treat established dyskinesia.

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More information

e-pub ahead of print date: 23 October 2013
Published date: February 2014
Keywords: Animals, Callithrix, Dyskinesias, Levodopa, MPTP Poisoning, Neostriatum, Proteome, Journal Article

Identifiers

Local EPrints ID: 416276
URI: http://eprints.soton.ac.uk/id/eprint/416276
ISSN: 0895-8696
PURE UUID: 085942c1-ae17-4371-b56a-6a8a6f8710dc

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Date deposited: 11 Dec 2017 17:30
Last modified: 15 Jan 2020 17:48

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Contributors

Author: Michael J. Jackson
Author: Lance A. Smith
Author: Sarah Rose
Author: Peter Jenner

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