NKCC1 (SLC12a2) induces a secondary axis in Xenopus laevis embryos independently of its co-transporter function
NKCC1 (SLC12a2) induces a secondary axis in Xenopus laevis embryos independently of its co-transporter function
NKCC1 is a broadly expressed Na(+)-K(+)-Cl(-) co-transporter involved in regulation of ion flux across the cell membrane and in regulating cell volume. Whilst much is known about the co-transporter activity of NKCC1 and its regulation by protein kinases and phosphatases, little is known about the activities of NKCC1 that are co-transporter independent. In this report we show that over-expression of NKCC1 in embryos of Xenopus laevis induces secondary axes, independently of its co-transporter activity. In addition, over-expression of NKCC1 results in the formation of neural tissue in ectodermal explants. We also show that NKCC1 is expressed broadly but non-uniformly in embryos of Xenopus laevis and Xenopus tropicalis, with prominent expression in the notochord, nervous system and stomach. These results provide insights into an additional, previously unreported activity of NKCCl.
Animals, Body Patterning, Bone Morphogenetic Proteins, Bumetanide, Cell Culture Techniques, Ectoderm, Furosemide, Gene Expression Profiling, Ion Transport, Nervous System, Notochord, Protein Structure, Tertiary, Sodium Potassium Chloride Symporter Inhibitors, Sodium-Potassium-Chloride Symporters, Solute Carrier Family 12, Member 2, Stomach, Wnt Proteins, Xenopus laevis, Journal Article, Research Support, Non-U.S. Gov't
521-529
Walters, Zoë S.
e1ccd35d-63a9-4951-a5da-59122193740d
Haworth, Kim E.
14a50baa-fe72-47dc-b987-7d21c53b6696
Latinkic, Branko V.
8620229f-5910-4412-b070-edde3fc75c7a
1 February 2009
Walters, Zoë S.
e1ccd35d-63a9-4951-a5da-59122193740d
Haworth, Kim E.
14a50baa-fe72-47dc-b987-7d21c53b6696
Latinkic, Branko V.
8620229f-5910-4412-b070-edde3fc75c7a
Walters, Zoë S., Haworth, Kim E. and Latinkic, Branko V.
(2009)
NKCC1 (SLC12a2) induces a secondary axis in Xenopus laevis embryos independently of its co-transporter function.
The Journal of Physiology, 587 (3), .
(doi:10.1113/jphysiol.2008.161562).
Abstract
NKCC1 is a broadly expressed Na(+)-K(+)-Cl(-) co-transporter involved in regulation of ion flux across the cell membrane and in regulating cell volume. Whilst much is known about the co-transporter activity of NKCC1 and its regulation by protein kinases and phosphatases, little is known about the activities of NKCC1 that are co-transporter independent. In this report we show that over-expression of NKCC1 in embryos of Xenopus laevis induces secondary axes, independently of its co-transporter activity. In addition, over-expression of NKCC1 results in the formation of neural tissue in ectodermal explants. We also show that NKCC1 is expressed broadly but non-uniformly in embryos of Xenopus laevis and Xenopus tropicalis, with prominent expression in the notochord, nervous system and stomach. These results provide insights into an additional, previously unreported activity of NKCCl.
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Accepted/In Press date: 28 November 2008
e-pub ahead of print date: 30 January 2009
Published date: 1 February 2009
Keywords:
Animals, Body Patterning, Bone Morphogenetic Proteins, Bumetanide, Cell Culture Techniques, Ectoderm, Furosemide, Gene Expression Profiling, Ion Transport, Nervous System, Notochord, Protein Structure, Tertiary, Sodium Potassium Chloride Symporter Inhibitors, Sodium-Potassium-Chloride Symporters, Solute Carrier Family 12, Member 2, Stomach, Wnt Proteins, Xenopus laevis, Journal Article, Research Support, Non-U.S. Gov't
Identifiers
Local EPrints ID: 416297
URI: http://eprints.soton.ac.uk/id/eprint/416297
ISSN: 0022-3751
PURE UUID: df6b09eb-46e6-45af-bf30-dab2019904c9
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Date deposited: 12 Dec 2017 17:30
Last modified: 16 Mar 2024 04:32
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Author:
Kim E. Haworth
Author:
Branko V. Latinkic
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