Identification of in vivo-expressed antigens of Staphylococcus aureus and their use in vaccinations for protection against nasal carriage
Identification of in vivo-expressed antigens of Staphylococcus aureus and their use in vaccinations for protection against nasal carriage
A spectrum of in vivo-expressed Staphylococcus aureus antigens was identified by probing bacteriophage expression libraries of S. aureus with serum samples from infected and uninfected individuals. Eleven recombinant antigenic proteins were produced, and specific antibody titers in a large collection of human serum samples were determined. Significantly increased concentrations of reactive immunoglobulin G (IgG) to 7 antigens were found in serum samples from ill individuals, compared with those in healthy individuals. Significantly higher concentrations of reactive IgG to 4 antigens, including iron-responsive surface determinant (Isd) A and IsdH, were found in serum samples from healthy individuals who were not nasal carriers of S. aureus, compared with those in healthy carriers. Vaccination of cotton rats with IsdA or IsdH protected against nasal carriage. Also, IsdA is involved in adherence of S. aureus to human desquamated nasal epithelial cells and is required for nasal colonization in the cotton rat model. Thus, vaccination with these antigens may prevent S. aureus carriage and reduce the prevalence of human disease.
Animals, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Vaccines, Carrier State, Cation Transport Proteins, Cells, Cultured, DNA Primers, Epithelial Cells, Female, Humans, Immunoglobulin G, Nasal Cavity, Receptors, Cell Surface, Recombinant Proteins, Sigmodontinae, Staphylococcal Infections, Staphylococcus aureus, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
1098-1108
Clarke, Simon R.
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Brummell, Kirsten J.
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Horsburgh, Malcolm J.
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McDowell, Philip W.
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Mohamad, Sharifah A. Syed
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Stapleton, Melanie R.
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Acevedo, Jorge
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Read, Robert C.
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Day, Nicholas P.J.
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Peacock, Sharon J.
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Mond, James J.
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Kokai-Kun, John F.
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Foster, Simon J.
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15 April 2006
Clarke, Simon R.
c59a1da9-60ca-4a72-a204-7412266c9741
Brummell, Kirsten J.
fccbd31f-d744-4e4a-ae5d-1e7903d9973c
Horsburgh, Malcolm J.
a3c23855-f354-4df5-aea6-bb966692563b
McDowell, Philip W.
34e0911f-c0e0-45d8-8ca0-b31323e217fd
Mohamad, Sharifah A. Syed
8c30d97a-e3ee-461e-83ff-554b53d423d7
Stapleton, Melanie R.
f0e937d3-51dc-4b00-b909-4c8aa41afd8c
Acevedo, Jorge
05ff5d06-c26e-407f-b1b7-6196e5767713
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Day, Nicholas P.J.
5221f169-4d82-465e-880f-dfce8f2e2bce
Peacock, Sharon J.
23cf95d2-0dac-48d0-a5c8-199114942afa
Mond, James J.
ce432f85-8e42-4267-a478-7a82a82cd326
Kokai-Kun, John F.
fb652306-2074-4c42-8dbd-4c3e7d69752a
Foster, Simon J.
6266b6db-9700-49c0-bee5-da9f52ca243a
Clarke, Simon R., Brummell, Kirsten J., Horsburgh, Malcolm J., McDowell, Philip W., Mohamad, Sharifah A. Syed, Stapleton, Melanie R., Acevedo, Jorge, Read, Robert C., Day, Nicholas P.J., Peacock, Sharon J., Mond, James J., Kokai-Kun, John F. and Foster, Simon J.
(2006)
Identification of in vivo-expressed antigens of Staphylococcus aureus and their use in vaccinations for protection against nasal carriage.
The Journal of Infectious Diseases, 193 (8), .
(doi:10.1086/501471).
Abstract
A spectrum of in vivo-expressed Staphylococcus aureus antigens was identified by probing bacteriophage expression libraries of S. aureus with serum samples from infected and uninfected individuals. Eleven recombinant antigenic proteins were produced, and specific antibody titers in a large collection of human serum samples were determined. Significantly increased concentrations of reactive immunoglobulin G (IgG) to 7 antigens were found in serum samples from ill individuals, compared with those in healthy individuals. Significantly higher concentrations of reactive IgG to 4 antigens, including iron-responsive surface determinant (Isd) A and IsdH, were found in serum samples from healthy individuals who were not nasal carriers of S. aureus, compared with those in healthy carriers. Vaccination of cotton rats with IsdA or IsdH protected against nasal carriage. Also, IsdA is involved in adherence of S. aureus to human desquamated nasal epithelial cells and is required for nasal colonization in the cotton rat model. Thus, vaccination with these antigens may prevent S. aureus carriage and reduce the prevalence of human disease.
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Accepted/In Press date: 16 November 2005
e-pub ahead of print date: 15 April 2006
Published date: 15 April 2006
Keywords:
Animals, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Vaccines, Carrier State, Cation Transport Proteins, Cells, Cultured, DNA Primers, Epithelial Cells, Female, Humans, Immunoglobulin G, Nasal Cavity, Receptors, Cell Surface, Recombinant Proteins, Sigmodontinae, Staphylococcal Infections, Staphylococcus aureus, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
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Local EPrints ID: 416311
URI: http://eprints.soton.ac.uk/id/eprint/416311
ISSN: 0022-1899
PURE UUID: b22a1cb0-e9b4-43ca-b7d0-7279570f182f
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Date deposited: 12 Dec 2017 17:30
Last modified: 16 Mar 2024 04:10
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Contributors
Author:
Simon R. Clarke
Author:
Kirsten J. Brummell
Author:
Malcolm J. Horsburgh
Author:
Philip W. McDowell
Author:
Sharifah A. Syed Mohamad
Author:
Melanie R. Stapleton
Author:
Jorge Acevedo
Author:
Nicholas P.J. Day
Author:
Sharon J. Peacock
Author:
James J. Mond
Author:
John F. Kokai-Kun
Author:
Simon J. Foster
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