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Potentiating angiogenesis arrest in vivo via laser irradiation of peptide functionalised gold nanoparticles

Potentiating angiogenesis arrest in vivo via laser irradiation of peptide functionalised gold nanoparticles
Potentiating angiogenesis arrest in vivo via laser irradiation of peptide functionalised gold nanoparticles

Background: Anti-angiogenic therapy has great potential for cancer therapy with several FDA approved formulations but there are considerable side effects upon the normal blood vessels that decrease the potential application of such therapeutics. Chicken chorioallantoic membrane (CAM) has been used as a model to study angiogenesis in vivo. Using a CAM model, it had been previously shown that spherical gold nanoparticles functionalised with an anti-angiogenic peptide can humper neo-angiogenesis.

Results: Our results show that gold nanoparticles conjugated with an anti-angiogenic peptide can be combined with visible laser irradiation to enhance angiogenesis arrest in vivo. We show that a green laser coupled to gold nanoparticles can achieve high localized temperatures able to precisely cauterize blood vessels. This combined therapy acts via VEGFR pathway inhibition, leading to a fourfold reduction in FLT-1 expression.

Conclusions: The proposed phototherapy extends the use of visible lasers in clinics, combining it with chemotherapy to potentiate cancer treatment. This approach allows the reduction of dose of anti-angiogenic peptide, thus reducing possible side effects, while destroying blood vessels supply critical for tumour progression.
1477-3155
1-8
Pedrosa, Pedro
4e26f035-75f1-45c0-a6ff-0a879b52b2fd
Heuer-Jungemann, Amelie
48f01a95-9ba4-4248-bd24-0e20d601d2cc
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Fernandes, Alexandra R.
2c686d7b-dd0b-4eb0-8731-6bff67106966
Baptista, Pedro V.
d2fbef22-44c4-4691-a54c-29415e7e5950
Pedrosa, Pedro
4e26f035-75f1-45c0-a6ff-0a879b52b2fd
Heuer-Jungemann, Amelie
48f01a95-9ba4-4248-bd24-0e20d601d2cc
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Fernandes, Alexandra R.
2c686d7b-dd0b-4eb0-8731-6bff67106966
Baptista, Pedro V.
d2fbef22-44c4-4691-a54c-29415e7e5950

Pedrosa, Pedro, Heuer-Jungemann, Amelie, Kanaras, Antonios G., Fernandes, Alexandra R. and Baptista, Pedro V. (2017) Potentiating angiogenesis arrest in vivo via laser irradiation of peptide functionalised gold nanoparticles. Journal of Nanobiotechnology, 85 (15), 1-8. (doi:10.1186/s12951-017-0321-2).

Record type: Article

Abstract


Background: Anti-angiogenic therapy has great potential for cancer therapy with several FDA approved formulations but there are considerable side effects upon the normal blood vessels that decrease the potential application of such therapeutics. Chicken chorioallantoic membrane (CAM) has been used as a model to study angiogenesis in vivo. Using a CAM model, it had been previously shown that spherical gold nanoparticles functionalised with an anti-angiogenic peptide can humper neo-angiogenesis.

Results: Our results show that gold nanoparticles conjugated with an anti-angiogenic peptide can be combined with visible laser irradiation to enhance angiogenesis arrest in vivo. We show that a green laser coupled to gold nanoparticles can achieve high localized temperatures able to precisely cauterize blood vessels. This combined therapy acts via VEGFR pathway inhibition, leading to a fourfold reduction in FLT-1 expression.

Conclusions: The proposed phototherapy extends the use of visible lasers in clinics, combining it with chemotherapy to potentiate cancer treatment. This approach allows the reduction of dose of anti-angiogenic peptide, thus reducing possible side effects, while destroying blood vessels supply critical for tumour progression.

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Accepted/In Press date: 14 November 2017
e-pub ahead of print date: 21 November 2017
Published date: November 2017

Identifiers

Local EPrints ID: 416337
URI: https://eprints.soton.ac.uk/id/eprint/416337
ISSN: 1477-3155
PURE UUID: 3621efb5-4d59-43ec-b7f3-1fb2c0130230
ORCID for Antonios G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

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Date deposited: 13 Dec 2017 17:30
Last modified: 14 Mar 2019 01:39

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