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Reduced interleukin-8 response to Streptococcus pneumoniae by alveolar macrophages from adults with HIV/AIDS

Reduced interleukin-8 response to Streptococcus pneumoniae by alveolar macrophages from adults with HIV/AIDS
Reduced interleukin-8 response to Streptococcus pneumoniae by alveolar macrophages from adults with HIV/AIDS

BACKGROUND: HIV-infected adults are highly susceptible to pneumococcal disease.

OBJECTIVE: To examine if alveolar macrophages from HIV-infected subjects exhibited a failure of cytokine production in response to Streptococcus pneumoniae in vitro.

DESIGN: Case-control comparison of alveolar macrophages from 11 HIV-infected and 13 non-infected adults.

METHODS: Type 1 opsonized S. pneumoniae were used to challenge the alveolar macrophages in vitro. Cell supernatant fluid was collected from unstimulated cells, and cells challenged with bacteria for 0, 6, 12 and 24 h. Cytokine production (interleukins 1beta, 6 and 8) was measured in all fluids using an enzyme-linked immunosorbent assay.

RESULTS: All the cytokines tested increased over time in both HIV-infected and uninfected subjects. Interleukin-8 release was significantly lower in HIV-infected than in non-HIV-infected subjects (P = 0.02).

CONCLUSION: Reduced interleukin-8 production may result in decreased neutrophil recruitment, and hence increased susceptibility to pneumococcal infection in HIV-infected subjects.

Adult, Case-Control Studies, Female, HIV Infections, Humans, Interleukin-8, Macrophages, Alveolar, Male, Middle Aged, Pneumococcal Infections, Streptococcus pneumoniae, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
0269-9370
1197-1200
Gordon, Stephen B.
e1d3f391-d2b9-4a4f-b7af-ad91cd29d48e
Jarman, Elizabeth R.
854e401d-5e8e-44aa-905b-1c013cf2db50
Kanyanda, Stonard
c6e306c0-22ef-445c-a286-e11b6e90dcd9
French, Neil
b30e68e2-12b1-4b6c-b9ed-70170090d6de
Pridmore, Alison C.
b704c202-afcc-4fde-9169-48e51f9fa0b0
Zijlstra, Eduard E.
6a049b30-e92d-4ff0-b40f-56ec64979ea6
Molyneux, Malcolm E.
2da5a12a-1c16-49bc-9b13-c068029e9676
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Gordon, Stephen B.
e1d3f391-d2b9-4a4f-b7af-ad91cd29d48e
Jarman, Elizabeth R.
854e401d-5e8e-44aa-905b-1c013cf2db50
Kanyanda, Stonard
c6e306c0-22ef-445c-a286-e11b6e90dcd9
French, Neil
b30e68e2-12b1-4b6c-b9ed-70170090d6de
Pridmore, Alison C.
b704c202-afcc-4fde-9169-48e51f9fa0b0
Zijlstra, Eduard E.
6a049b30-e92d-4ff0-b40f-56ec64979ea6
Molyneux, Malcolm E.
2da5a12a-1c16-49bc-9b13-c068029e9676
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51

Gordon, Stephen B., Jarman, Elizabeth R., Kanyanda, Stonard, French, Neil, Pridmore, Alison C., Zijlstra, Eduard E., Molyneux, Malcolm E. and Read, Robert C. (2005) Reduced interleukin-8 response to Streptococcus pneumoniae by alveolar macrophages from adults with HIV/AIDS. AIDS, 19 (11), 1197-1200. (doi:10.1097/01.aids.0000176220.25614.5e).

Record type: Article

Abstract

BACKGROUND: HIV-infected adults are highly susceptible to pneumococcal disease.

OBJECTIVE: To examine if alveolar macrophages from HIV-infected subjects exhibited a failure of cytokine production in response to Streptococcus pneumoniae in vitro.

DESIGN: Case-control comparison of alveolar macrophages from 11 HIV-infected and 13 non-infected adults.

METHODS: Type 1 opsonized S. pneumoniae were used to challenge the alveolar macrophages in vitro. Cell supernatant fluid was collected from unstimulated cells, and cells challenged with bacteria for 0, 6, 12 and 24 h. Cytokine production (interleukins 1beta, 6 and 8) was measured in all fluids using an enzyme-linked immunosorbent assay.

RESULTS: All the cytokines tested increased over time in both HIV-infected and uninfected subjects. Interleukin-8 release was significantly lower in HIV-infected than in non-HIV-infected subjects (P = 0.02).

CONCLUSION: Reduced interleukin-8 production may result in decreased neutrophil recruitment, and hence increased susceptibility to pneumococcal infection in HIV-infected subjects.

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More information

Published date: 22 July 2005
Keywords: Adult, Case-Control Studies, Female, HIV Infections, Humans, Interleukin-8, Macrophages, Alveolar, Male, Middle Aged, Pneumococcal Infections, Streptococcus pneumoniae, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416380
URI: http://eprints.soton.ac.uk/id/eprint/416380
ISSN: 0269-9370
PURE UUID: 17507014-075f-4f1d-9df9-b25f8c7d2a0c
ORCID for Robert C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 14 Dec 2017 17:30
Last modified: 10 Nov 2021 03:30

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Contributors

Author: Stephen B. Gordon
Author: Elizabeth R. Jarman
Author: Stonard Kanyanda
Author: Neil French
Author: Alison C. Pridmore
Author: Eduard E. Zijlstra
Author: Malcolm E. Molyneux
Author: Robert C. Read ORCID iD

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