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Induction of pro-inflammatory cytokine release by human macrophages during exposure of Streptococcus pneumoniae to penicillin is influenced by minimum inhibitory concentration ratio

Induction of pro-inflammatory cytokine release by human macrophages during exposure of Streptococcus pneumoniae to penicillin is influenced by minimum inhibitory concentration ratio
Induction of pro-inflammatory cytokine release by human macrophages during exposure of Streptococcus pneumoniae to penicillin is influenced by minimum inhibitory concentration ratio

beta-Lactam antibiotics cause release of pro-inflammatory bacterial cell wall structures. We determined the effect of penicillin treatment of Streptococcus pneumoniae on the induction of tumour necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) genes by human macrophages and the influence of antibiotic concentration and bacterial growth phase upon this induction. Gene expression was measured by real-time polymerase chain reaction (PCR) and protein was measured by enzyme-linked immunosorbent assay (ELISA). Treatment of lag phase S. pneumoniae with one-eighth minimum inhibitory concentration (MIC) penicillin resulted in enhanced expression of TNF-alpha messenger RNA (mRNA), but not TNF-alpha protein at 6h compared with untreated bacteria. IL-1beta mRNA and protein were not affected by these bacteria. MIC treatment of lag or early log phase bacteria induced both protein and mRNA for IL-1beta. Bacteria exposed to concentrations of penicillin that cause lysis (MIC) or no lysis with morphological changes (sub-MIC) induce differential patterns of pro-inflammatory cytokine expression by human macrophages.

Anti-Bacterial Agents, Cells, Cultured, Cytokines, Enzyme-Linked Immunosorbent Assay, Gene Expression, Humans, Interleukin-1, Macrophages, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Penicillins, Polymerase Chain Reaction, RNA, Messenger, Streptococcus pneumoniae, Tumor Necrosis Factor-alpha, Journal Article, Research Support, Non-U.S. Gov't
0924-8579
188-196
Moore, Lisa J.
13095db5-12ee-47f4-ae09-b1deca4881d3
Pridmore, Alison C.
b704c202-afcc-4fde-9169-48e51f9fa0b0
Lee, Margaret E.
a87d108c-c8e3-420c-be05-e7e23ee0619e
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Moore, Lisa J.
13095db5-12ee-47f4-ae09-b1deca4881d3
Pridmore, Alison C.
b704c202-afcc-4fde-9169-48e51f9fa0b0
Lee, Margaret E.
a87d108c-c8e3-420c-be05-e7e23ee0619e
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51

Moore, Lisa J., Pridmore, Alison C., Lee, Margaret E. and Read, Robert C. (2005) Induction of pro-inflammatory cytokine release by human macrophages during exposure of Streptococcus pneumoniae to penicillin is influenced by minimum inhibitory concentration ratio. International Journal of Antimicrobial Agents, 26 (3), 188-196. (doi:10.1016/j.ijantimicag.2005.06.006).

Record type: Article

Abstract

beta-Lactam antibiotics cause release of pro-inflammatory bacterial cell wall structures. We determined the effect of penicillin treatment of Streptococcus pneumoniae on the induction of tumour necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) genes by human macrophages and the influence of antibiotic concentration and bacterial growth phase upon this induction. Gene expression was measured by real-time polymerase chain reaction (PCR) and protein was measured by enzyme-linked immunosorbent assay (ELISA). Treatment of lag phase S. pneumoniae with one-eighth minimum inhibitory concentration (MIC) penicillin resulted in enhanced expression of TNF-alpha messenger RNA (mRNA), but not TNF-alpha protein at 6h compared with untreated bacteria. IL-1beta mRNA and protein were not affected by these bacteria. MIC treatment of lag or early log phase bacteria induced both protein and mRNA for IL-1beta. Bacteria exposed to concentrations of penicillin that cause lysis (MIC) or no lysis with morphological changes (sub-MIC) induce differential patterns of pro-inflammatory cytokine expression by human macrophages.

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More information

Published date: September 2005
Keywords: Anti-Bacterial Agents, Cells, Cultured, Cytokines, Enzyme-Linked Immunosorbent Assay, Gene Expression, Humans, Interleukin-1, Macrophages, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Penicillins, Polymerase Chain Reaction, RNA, Messenger, Streptococcus pneumoniae, Tumor Necrosis Factor-alpha, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416381
URI: http://eprints.soton.ac.uk/id/eprint/416381
ISSN: 0924-8579
PURE UUID: 7f038b4f-dc95-41a5-a41c-62f436da5344
ORCID for Robert C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 14 Dec 2017 17:30
Last modified: 10 Nov 2021 03:30

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Contributors

Author: Lisa J. Moore
Author: Alison C. Pridmore
Author: Margaret E. Lee
Author: Robert C. Read ORCID iD

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