The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

Penicillin enhances the toll-like receptor 2-mediated proinflammatory activity of Streptococcus pneumoniae

Penicillin enhances the toll-like receptor 2-mediated proinflammatory activity of Streptococcus pneumoniae
Penicillin enhances the toll-like receptor 2-mediated proinflammatory activity of Streptococcus pneumoniae

The Streptococcus pneumoniae cell-wall components peptidoglycan and lipoteichoic acid activate Toll-like receptor 2 (TLR2), which transduces an inflammatory response. After exposure to penicillin, type 2 S. pneumoniae strain D39, but not the isogenic autolysin-deficient mutant AL2, induced significantly enhanced interleukin-8 promoter activity in TLR2-transfected HeLa cells. Lag-phase D39 exhibited enhanced TLR2 activation after exposure to penicillin at levels below the minimum inhibitory concentration (MIC); in contrast, early log-phase S. pneumoniae were most active when exposed to the MIC. This enhancement was not ablated by heat treatment but was attenuated by autolysin inhibitors. The antimicrobial activity of moxifloxacin and erythromycin was not associated with TLR2 activation by S. pneumoniae. These data show that penicillin treatment of S. pneumoniae releases proinflammatory cell-wall components that activate TLR2 and that this activity is dependent on autolysin, the growth phase of the organism, and the antibiotic concentration.

Anti-Infective Agents, Aza Compounds, Choline, Colony Count, Microbial, Enzyme Inhibitors, Erythromycin, Ethanolamine, Fluoroquinolones, HeLa Cells, Humans, Interleukin-8, Membrane Glycoproteins, N-Acetylmuramoyl-L-alanine Amidase, Penicillins, Pneumococcal Infections, Quinolines, Receptors, Cell Surface, Streptococcus pneumoniae, Toll-Like Receptor 2, Toll-Like Receptors, Transfection, Journal Article, Research Support, Non-U.S. Gov't
0022-1899
1040-1048
Moore, Lisa J.
13095db5-12ee-47f4-ae09-b1deca4881d3
Pridmore, Alison C.
b704c202-afcc-4fde-9169-48e51f9fa0b0
Dower, Steven K.
c59b6ed1-57d4-49cb-815c-038a96601ecc
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Moore, Lisa J.
13095db5-12ee-47f4-ae09-b1deca4881d3
Pridmore, Alison C.
b704c202-afcc-4fde-9169-48e51f9fa0b0
Dower, Steven K.
c59b6ed1-57d4-49cb-815c-038a96601ecc
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51

Moore, Lisa J., Pridmore, Alison C., Dower, Steven K. and Read, Robert C. (2003) Penicillin enhances the toll-like receptor 2-mediated proinflammatory activity of Streptococcus pneumoniae. The Journal of Infectious Diseases, 188 (7), 1040-1048. (doi:10.1086/378238).

Record type: Article

Abstract

The Streptococcus pneumoniae cell-wall components peptidoglycan and lipoteichoic acid activate Toll-like receptor 2 (TLR2), which transduces an inflammatory response. After exposure to penicillin, type 2 S. pneumoniae strain D39, but not the isogenic autolysin-deficient mutant AL2, induced significantly enhanced interleukin-8 promoter activity in TLR2-transfected HeLa cells. Lag-phase D39 exhibited enhanced TLR2 activation after exposure to penicillin at levels below the minimum inhibitory concentration (MIC); in contrast, early log-phase S. pneumoniae were most active when exposed to the MIC. This enhancement was not ablated by heat treatment but was attenuated by autolysin inhibitors. The antimicrobial activity of moxifloxacin and erythromycin was not associated with TLR2 activation by S. pneumoniae. These data show that penicillin treatment of S. pneumoniae releases proinflammatory cell-wall components that activate TLR2 and that this activity is dependent on autolysin, the growth phase of the organism, and the antibiotic concentration.

This record has no associated files available for download.

More information

Published date: 1 October 2003
Keywords: Anti-Infective Agents, Aza Compounds, Choline, Colony Count, Microbial, Enzyme Inhibitors, Erythromycin, Ethanolamine, Fluoroquinolones, HeLa Cells, Humans, Interleukin-8, Membrane Glycoproteins, N-Acetylmuramoyl-L-alanine Amidase, Penicillins, Pneumococcal Infections, Quinolines, Receptors, Cell Surface, Streptococcus pneumoniae, Toll-Like Receptor 2, Toll-Like Receptors, Transfection, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416419
URI: http://eprints.soton.ac.uk/id/eprint/416419
ISSN: 0022-1899
PURE UUID: 41444eab-e9b7-4c83-be9d-08ede0a7bbb9
ORCID for Robert C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 15 Dec 2017 17:30
Last modified: 10 Nov 2021 03:30

Export record

Altmetrics

Contributors

Author: Lisa J. Moore
Author: Alison C. Pridmore
Author: Steven K. Dower
Author: Robert C. Read ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×