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Streptococcus pneumoniae-associated human macrophage apoptosis after bacterial internalization via complement and Fcγ receptors correlates with intracellular bacterial load

Streptococcus pneumoniae-associated human macrophage apoptosis after bacterial internalization via complement and Fcγ receptors correlates with intracellular bacterial load
Streptococcus pneumoniae-associated human macrophage apoptosis after bacterial internalization via complement and Fcγ receptors correlates with intracellular bacterial load

Opsonization enhances Streptococcus pneumoniae-induced human monocyte-derived macrophage (MDM) apoptosis. Both depletion of complement and immunoglobulin from opsonizing serum and blockade of the macrophages CR1, CR3, FcgammaRII, and FcgammaRIII partially decreased MDM apoptosis after S. pneumoniae phagocytosis, and these effects correlated with reduced numbers of internalized bacteria. Chloramphenicol inhibition of protein synthesis by opsonized S. pneumoniae down-regulated subsequent MDM apoptosis. Phagocytosis of an unencapsulated mutant of S. pneumoniae resulted in increased MDM apoptosis, in association with enhanced internalization. Caspase inhibition was associated with decreased killing of bacteria. Enhanced induction of apoptosis by opsonized S. pneumoniae is the result of increased intracellular burden of bacteria, rather than of a specific pattern of engagement of complement receptor or FcgammaR. A dynamic interaction between live intracellular bacteria and the host cell is necessary for induction of apoptosis in MDMs, and induction of apoptosis contributes to the host defense against S. pneumoniae.

Apoptosis, Flow Cytometry, Humans, Macrophage Activation, Macrophages, Microscopy, Fluorescence, Monocytes, Opsonin Proteins, Phagocytosis, Pneumonia, Pneumococcal, Receptors, Complement, Receptors, IgG, Streptococcus pneumoniae, Journal Article, Research Support, Non-U.S. Gov't
0022-1899
1119-1131
Ali, Farzana
2bf00407-ff59-41e7-8fcf-547cd1dc4f6a
Lee, Margaret E.
a87d108c-c8e3-420c-be05-e7e23ee0619e
Iannelli, Francesco
0a415730-38ec-484e-81f3-a7a17d765619
Pozzi, Gianni
610f2b6b-ef5c-4419-9792-5ce57d27cb07
Mitchell, Tim J.
d11e17e1-428b-4b7c-a0f3-4e954ea0562c
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Dockrell, David H.
a068c9bf-35b8-4c10-8f91-58639cfeca0b
Ali, Farzana
2bf00407-ff59-41e7-8fcf-547cd1dc4f6a
Lee, Margaret E.
a87d108c-c8e3-420c-be05-e7e23ee0619e
Iannelli, Francesco
0a415730-38ec-484e-81f3-a7a17d765619
Pozzi, Gianni
610f2b6b-ef5c-4419-9792-5ce57d27cb07
Mitchell, Tim J.
d11e17e1-428b-4b7c-a0f3-4e954ea0562c
Read, Robert C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Dockrell, David H.
a068c9bf-35b8-4c10-8f91-58639cfeca0b

Ali, Farzana, Lee, Margaret E., Iannelli, Francesco, Pozzi, Gianni, Mitchell, Tim J., Read, Robert C. and Dockrell, David H. (2003) Streptococcus pneumoniae-associated human macrophage apoptosis after bacterial internalization via complement and Fcγ receptors correlates with intracellular bacterial load. The Journal of Infectious Diseases, 188 (8), 1119-1131. (doi:10.1086/378675).

Record type: Article

Abstract

Opsonization enhances Streptococcus pneumoniae-induced human monocyte-derived macrophage (MDM) apoptosis. Both depletion of complement and immunoglobulin from opsonizing serum and blockade of the macrophages CR1, CR3, FcgammaRII, and FcgammaRIII partially decreased MDM apoptosis after S. pneumoniae phagocytosis, and these effects correlated with reduced numbers of internalized bacteria. Chloramphenicol inhibition of protein synthesis by opsonized S. pneumoniae down-regulated subsequent MDM apoptosis. Phagocytosis of an unencapsulated mutant of S. pneumoniae resulted in increased MDM apoptosis, in association with enhanced internalization. Caspase inhibition was associated with decreased killing of bacteria. Enhanced induction of apoptosis by opsonized S. pneumoniae is the result of increased intracellular burden of bacteria, rather than of a specific pattern of engagement of complement receptor or FcgammaR. A dynamic interaction between live intracellular bacteria and the host cell is necessary for induction of apoptosis in MDMs, and induction of apoptosis contributes to the host defense against S. pneumoniae.

Full text not available from this repository.

More information

Published date: 15 October 2003
Keywords: Apoptosis, Flow Cytometry, Humans, Macrophage Activation, Macrophages, Microscopy, Fluorescence, Monocytes, Opsonin Proteins, Phagocytosis, Pneumonia, Pneumococcal, Receptors, Complement, Receptors, IgG, Streptococcus pneumoniae, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416420
URI: https://eprints.soton.ac.uk/id/eprint/416420
ISSN: 0022-1899
PURE UUID: 98fded67-4602-47bd-a2f6-7712b3a48e85
ORCID for Robert C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 15 Dec 2017 17:30
Last modified: 20 Jul 2019 00:42

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