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Invasion by Neisseria meningitidis varies widely between clones and among nasopharyngeal mucosae derived from adult human hosts

Invasion by Neisseria meningitidis varies widely between clones and among nasopharyngeal mucosae derived from adult human hosts
Invasion by Neisseria meningitidis varies widely between clones and among nasopharyngeal mucosae derived from adult human hosts

Colonization of the human nasopharynx is a feature of some species of Neisseria, and is a prerequisite of invasive meningococcal disease. The likelihood of colonization by Neisseria meningitidis varies widely between humans, and very few develop invasive disease. Explants of nasal mucosa derived from adult patients with non-allergic nasal obstruction were infected experimentally with Neisseria spp. At intervals over 18 h incubation, washed explants were homogenized, and viable bacteria were counted. To estimate bacterial invasion of mucosa, explants were exposed to 0.25% sodium taurocholate for 30 s prior to homogenization. N. meningitidis was recovered from the mucosa and the organism invaded and replicated within the tissue, in contrast to N. lactamica and N. animalis (n=9, P<0.008). N. meningitidis isolates of clones ET-5, ET-37 and lineage III were recovered from and invaded tissue, but strains of clones A4, A:subgroup I, A:subgroup III and A:subgroup IV-1 did not invade (n=6). To measure host variation, survival of N. meningitidis within nasal mucosa of 40 different human donors was measured. Intra-class correlation of replicates was 0.97, but the coefficient of variation of recovered viable counts was 1335% after 4 h and 77% after 18 h incubation. It is concluded that the distinctive colonization and disease potential of Neisseria spp. may be partly a consequence of their ability to invade and survive within human nasopharyngeal mucosa, but that this is influenced greatly by genetic or environmental factors operating on the host mucosa. This is consistent with the unpredictable epidemiology of meningococcal disease.

Adult, Blotting, Western, Clone Cells, Disease Susceptibility, Host-Parasite Interactions, Humans, Models, Biological, Nasal Mucosa, Nasopharynx, Neisseria meningitidis, Organ Culture Techniques, Reproducibility of Results, Time Factors, Journal Article, Research Support, Non-U.S. Gov't
1350-0872
1467-74
Townsend, Robert
4a7ce3d4-7d52-4f3c-b540-b5f94bbc3d5d
Goodwin, Linda
45c48038-ca4d-451a-9fbe-b4d6ea95048b
Stevanin, Tania M
f1504019-7365-4b69-8404-e9df63a5348a
Silcocks, Paul B
c02fb003-fe21-4172-abc0-27ebafe6df3e
Parker, Andrew
464e9c03-3592-4da4-b9c0-a45d3c56a785
Maiden, Martin C J
721886a4-7548-45c8-882f-7a9cae695c0c
Read, Robert C
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Townsend, Robert
4a7ce3d4-7d52-4f3c-b540-b5f94bbc3d5d
Goodwin, Linda
45c48038-ca4d-451a-9fbe-b4d6ea95048b
Stevanin, Tania M
f1504019-7365-4b69-8404-e9df63a5348a
Silcocks, Paul B
c02fb003-fe21-4172-abc0-27ebafe6df3e
Parker, Andrew
464e9c03-3592-4da4-b9c0-a45d3c56a785
Maiden, Martin C J
721886a4-7548-45c8-882f-7a9cae695c0c
Read, Robert C
b5caca7b-0063-438a-b703-7ecbb6fc2b51

Townsend, Robert, Goodwin, Linda, Stevanin, Tania M, Silcocks, Paul B, Parker, Andrew, Maiden, Martin C J and Read, Robert C (2002) Invasion by Neisseria meningitidis varies widely between clones and among nasopharyngeal mucosae derived from adult human hosts. Microbiology, 148 (Pt 5), 1467-74. (doi:10.1099/00221287-148-5-1467).

Record type: Article

Abstract

Colonization of the human nasopharynx is a feature of some species of Neisseria, and is a prerequisite of invasive meningococcal disease. The likelihood of colonization by Neisseria meningitidis varies widely between humans, and very few develop invasive disease. Explants of nasal mucosa derived from adult patients with non-allergic nasal obstruction were infected experimentally with Neisseria spp. At intervals over 18 h incubation, washed explants were homogenized, and viable bacteria were counted. To estimate bacterial invasion of mucosa, explants were exposed to 0.25% sodium taurocholate for 30 s prior to homogenization. N. meningitidis was recovered from the mucosa and the organism invaded and replicated within the tissue, in contrast to N. lactamica and N. animalis (n=9, P<0.008). N. meningitidis isolates of clones ET-5, ET-37 and lineage III were recovered from and invaded tissue, but strains of clones A4, A:subgroup I, A:subgroup III and A:subgroup IV-1 did not invade (n=6). To measure host variation, survival of N. meningitidis within nasal mucosa of 40 different human donors was measured. Intra-class correlation of replicates was 0.97, but the coefficient of variation of recovered viable counts was 1335% after 4 h and 77% after 18 h incubation. It is concluded that the distinctive colonization and disease potential of Neisseria spp. may be partly a consequence of their ability to invade and survive within human nasopharyngeal mucosa, but that this is influenced greatly by genetic or environmental factors operating on the host mucosa. This is consistent with the unpredictable epidemiology of meningococcal disease.

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More information

Published date: May 2002
Keywords: Adult, Blotting, Western, Clone Cells, Disease Susceptibility, Host-Parasite Interactions, Humans, Models, Biological, Nasal Mucosa, Nasopharynx, Neisseria meningitidis, Organ Culture Techniques, Reproducibility of Results, Time Factors, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416549
URI: http://eprints.soton.ac.uk/id/eprint/416549
ISSN: 1350-0872
PURE UUID: e081181d-48e3-4abb-a1f5-bf520525ec27
ORCID for Robert C Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 21 Dec 2017 17:30
Last modified: 10 Nov 2021 03:30

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Contributors

Author: Robert Townsend
Author: Linda Goodwin
Author: Tania M Stevanin
Author: Paul B Silcocks
Author: Andrew Parker
Author: Martin C J Maiden
Author: Robert C Read ORCID iD

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