Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells
Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells
Recruitment of neutrophils into alveolar air spaces is an early event in the pathogenesis of pneumonia due to Streptococcus pneumoniae. This results from chemokines released by activated endothelial and epithelial cells and alveolar macrophages. Culture supernatants of 6 wild-type strains of S. pneumoniae, shown to contain choline-binding protein A (CbpA; clades A and B), induced release of chemokine CXCL8 from the human alveolar epithelial cell line A549, whereas a CbpA deletion mutant elicited significantly reduced CXCL8 release, compared with that of its isogenic parent (P<.01). Recombinant CbpA up-regulated expression of messenger RNA of CXCL8 and CCL2 but not of XCL1, CXCL10, CCL1, CCL3, CCL4, or CCL5 in A549 cells and induced increased secretion of CXCL8, CCL2, CXCL1, and CXCL5 in a dose- and time-dependent manner. CbpA also increased the expression of intercellular adhesion molecule 1 (CD54) by A549 cells. Thus, CbpA of S. pneumoniae induces the transcription and release of proinflammatory molecules by human alveolar epithelial cells.
Antigens, Bacterial, Base Sequence, Cell Line, Chemokines, DNA Primers, Epithelial Cells, Gene Expression Regulation, Humans, Intercellular Adhesion Molecule-1, Polymerase Chain Reaction, Pulmonary Alveoli, Receptors, Chemokine, Streptococcus pneumoniae, Transcription, Genetic, Journal Article, Research Support, Non-U.S. Gov't
1253-1260
Murdoch, Craig
5408c59b-28c0-40e9-89fc-7be76ce18bb1
Read, Robert C
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Zhang, Qibo
930f1a8d-3fbf-446f-9a5f-47bc0f5a1d42
Finn, Adam
59ac74fa-55a6-448d-9e19-9dd2f4654d4b
1 November 2002
Murdoch, Craig
5408c59b-28c0-40e9-89fc-7be76ce18bb1
Read, Robert C
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Zhang, Qibo
930f1a8d-3fbf-446f-9a5f-47bc0f5a1d42
Finn, Adam
59ac74fa-55a6-448d-9e19-9dd2f4654d4b
Murdoch, Craig, Read, Robert C, Zhang, Qibo and Finn, Adam
(2002)
Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells.
The Journal of Infectious Diseases, 186 (9), .
(doi:10.1086/344527).
Abstract
Recruitment of neutrophils into alveolar air spaces is an early event in the pathogenesis of pneumonia due to Streptococcus pneumoniae. This results from chemokines released by activated endothelial and epithelial cells and alveolar macrophages. Culture supernatants of 6 wild-type strains of S. pneumoniae, shown to contain choline-binding protein A (CbpA; clades A and B), induced release of chemokine CXCL8 from the human alveolar epithelial cell line A549, whereas a CbpA deletion mutant elicited significantly reduced CXCL8 release, compared with that of its isogenic parent (P<.01). Recombinant CbpA up-regulated expression of messenger RNA of CXCL8 and CCL2 but not of XCL1, CXCL10, CCL1, CCL3, CCL4, or CCL5 in A549 cells and induced increased secretion of CXCL8, CCL2, CXCL1, and CXCL5 in a dose- and time-dependent manner. CbpA also increased the expression of intercellular adhesion molecule 1 (CD54) by A549 cells. Thus, CbpA of S. pneumoniae induces the transcription and release of proinflammatory molecules by human alveolar epithelial cells.
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Published date: 1 November 2002
Keywords:
Antigens, Bacterial, Base Sequence, Cell Line, Chemokines, DNA Primers, Epithelial Cells, Gene Expression Regulation, Humans, Intercellular Adhesion Molecule-1, Polymerase Chain Reaction, Pulmonary Alveoli, Receptors, Chemokine, Streptococcus pneumoniae, Transcription, Genetic, Journal Article, Research Support, Non-U.S. Gov't
Identifiers
Local EPrints ID: 416554
URI: http://eprints.soton.ac.uk/id/eprint/416554
ISSN: 0022-1899
PURE UUID: b93f5c78-31f2-45e9-8331-f1e2ed06a0cc
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Date deposited: 21 Dec 2017 17:30
Last modified: 16 Mar 2024 04:10
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Author:
Craig Murdoch
Author:
Qibo Zhang
Author:
Adam Finn
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