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Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells

Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells
Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells

Recruitment of neutrophils into alveolar air spaces is an early event in the pathogenesis of pneumonia due to Streptococcus pneumoniae. This results from chemokines released by activated endothelial and epithelial cells and alveolar macrophages. Culture supernatants of 6 wild-type strains of S. pneumoniae, shown to contain choline-binding protein A (CbpA; clades A and B), induced release of chemokine CXCL8 from the human alveolar epithelial cell line A549, whereas a CbpA deletion mutant elicited significantly reduced CXCL8 release, compared with that of its isogenic parent (P<.01). Recombinant CbpA up-regulated expression of messenger RNA of CXCL8 and CCL2 but not of XCL1, CXCL10, CCL1, CCL3, CCL4, or CCL5 in A549 cells and induced increased secretion of CXCL8, CCL2, CXCL1, and CXCL5 in a dose- and time-dependent manner. CbpA also increased the expression of intercellular adhesion molecule 1 (CD54) by A549 cells. Thus, CbpA of S. pneumoniae induces the transcription and release of proinflammatory molecules by human alveolar epithelial cells.

Antigens, Bacterial, Base Sequence, Cell Line, Chemokines, DNA Primers, Epithelial Cells, Gene Expression Regulation, Humans, Intercellular Adhesion Molecule-1, Polymerase Chain Reaction, Pulmonary Alveoli, Receptors, Chemokine, Streptococcus pneumoniae, Transcription, Genetic, Journal Article, Research Support, Non-U.S. Gov't
0022-1899
1253-1260
Murdoch, Craig
5408c59b-28c0-40e9-89fc-7be76ce18bb1
Read, Robert C
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Zhang, Qibo
930f1a8d-3fbf-446f-9a5f-47bc0f5a1d42
Finn, Adam
59ac74fa-55a6-448d-9e19-9dd2f4654d4b
Murdoch, Craig
5408c59b-28c0-40e9-89fc-7be76ce18bb1
Read, Robert C
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Zhang, Qibo
930f1a8d-3fbf-446f-9a5f-47bc0f5a1d42
Finn, Adam
59ac74fa-55a6-448d-9e19-9dd2f4654d4b

Murdoch, Craig, Read, Robert C, Zhang, Qibo and Finn, Adam (2002) Choline-binding protein A of Streptococcus pneumoniae elicits chemokine production and expression of intercellular adhesion molecule 1 (CD54) by human alveolar epithelial cells. The Journal of Infectious Diseases, 186 (9), 1253-1260. (doi:10.1086/344527).

Record type: Article

Abstract

Recruitment of neutrophils into alveolar air spaces is an early event in the pathogenesis of pneumonia due to Streptococcus pneumoniae. This results from chemokines released by activated endothelial and epithelial cells and alveolar macrophages. Culture supernatants of 6 wild-type strains of S. pneumoniae, shown to contain choline-binding protein A (CbpA; clades A and B), induced release of chemokine CXCL8 from the human alveolar epithelial cell line A549, whereas a CbpA deletion mutant elicited significantly reduced CXCL8 release, compared with that of its isogenic parent (P<.01). Recombinant CbpA up-regulated expression of messenger RNA of CXCL8 and CCL2 but not of XCL1, CXCL10, CCL1, CCL3, CCL4, or CCL5 in A549 cells and induced increased secretion of CXCL8, CCL2, CXCL1, and CXCL5 in a dose- and time-dependent manner. CbpA also increased the expression of intercellular adhesion molecule 1 (CD54) by A549 cells. Thus, CbpA of S. pneumoniae induces the transcription and release of proinflammatory molecules by human alveolar epithelial cells.

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More information

Published date: 1 November 2002
Keywords: Antigens, Bacterial, Base Sequence, Cell Line, Chemokines, DNA Primers, Epithelial Cells, Gene Expression Regulation, Humans, Intercellular Adhesion Molecule-1, Polymerase Chain Reaction, Pulmonary Alveoli, Receptors, Chemokine, Streptococcus pneumoniae, Transcription, Genetic, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416554
URI: http://eprints.soton.ac.uk/id/eprint/416554
ISSN: 0022-1899
PURE UUID: b93f5c78-31f2-45e9-8331-f1e2ed06a0cc
ORCID for Robert C Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 21 Dec 2017 17:30
Last modified: 10 Nov 2021 03:30

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Contributors

Author: Craig Murdoch
Author: Robert C Read ORCID iD
Author: Qibo Zhang
Author: Adam Finn

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