The University of Southampton
University of Southampton Institutional Repository

Immune-mediated phagocytosis and killing of Streptococcus pneumoniae are associated with direct and bystander macrophage apoptosis

Immune-mediated phagocytosis and killing of Streptococcus pneumoniae are associated with direct and bystander macrophage apoptosis
Immune-mediated phagocytosis and killing of Streptococcus pneumoniae are associated with direct and bystander macrophage apoptosis

Apoptosis of macrophages may be a pathogen-directed mechanism of immune escape or may represent appropriate host response to infection. Human monocyte-derived macrophages (MDMs) from healthy donors (C-MDMs) exhibited low-level constitutive apoptosis, but culture of MDMs with opsonized serotype I Streptococcus pneumoniae (I-MDMs) for 20 h resulted in significantly increased apoptosis. I-MDM apoptosis was associated with phagocytosis of bacteria and intracellular killing that was blocked by the caspase inhibitor z-VAD-fmk but not by Fas-blocking antibody. Paraformaldehyde-fixed I-MDMs induced apoptosis in uninfected syngeneic monocytes at levels greater than those in monocytes incubated alone or incubated with fixed C-MDMs. Apoptosis of syngeneic monocytes was blocked by anti-Fas antibody. The immune response of macrophages to S. pneumoniae includes a novel form of apoptosis that is associated with successful phagocytosis and bacterial killing. This response in vivo may regulate the inflammatory response to infection during a successful host response against S. pneumoniae.

Amino Acid Chloromethyl Ketones, Apoptosis, Caspase Inhibitors, Cells, Cultured, Cysteine Proteinase Inhibitors, Humans, In Situ Nick-End Labeling, Macrophages, Monocytes, Phagocytosis, Reference Values, Regression Analysis, Streptococcus pneumoniae, Time Factors, Journal Article, Research Support, Non-U.S. Gov't
0022-1899
713-722
Dockrell, D.H.
df2521bf-534f-4798-9b1d-1c02f7e72713
Lee, M.
d0e603c6-91c1-44f3-952e-88d38aa4191c
Lynch, D.H.
de3d7fdd-f43d-45af-8bb1-4a1ce2843f96
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Dockrell, D.H.
df2521bf-534f-4798-9b1d-1c02f7e72713
Lee, M.
d0e603c6-91c1-44f3-952e-88d38aa4191c
Lynch, D.H.
de3d7fdd-f43d-45af-8bb1-4a1ce2843f96
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51

Dockrell, D.H., Lee, M., Lynch, D.H. and Read, R.C. (2001) Immune-mediated phagocytosis and killing of Streptococcus pneumoniae are associated with direct and bystander macrophage apoptosis. The Journal of Infectious Diseases, 184 (6), 713-722. (doi:10.1086/323084).

Record type: Article

Abstract

Apoptosis of macrophages may be a pathogen-directed mechanism of immune escape or may represent appropriate host response to infection. Human monocyte-derived macrophages (MDMs) from healthy donors (C-MDMs) exhibited low-level constitutive apoptosis, but culture of MDMs with opsonized serotype I Streptococcus pneumoniae (I-MDMs) for 20 h resulted in significantly increased apoptosis. I-MDM apoptosis was associated with phagocytosis of bacteria and intracellular killing that was blocked by the caspase inhibitor z-VAD-fmk but not by Fas-blocking antibody. Paraformaldehyde-fixed I-MDMs induced apoptosis in uninfected syngeneic monocytes at levels greater than those in monocytes incubated alone or incubated with fixed C-MDMs. Apoptosis of syngeneic monocytes was blocked by anti-Fas antibody. The immune response of macrophages to S. pneumoniae includes a novel form of apoptosis that is associated with successful phagocytosis and bacterial killing. This response in vivo may regulate the inflammatory response to infection during a successful host response against S. pneumoniae.

This record has no associated files available for download.

More information

Published date: 15 September 2001
Keywords: Amino Acid Chloromethyl Ketones, Apoptosis, Caspase Inhibitors, Cells, Cultured, Cysteine Proteinase Inhibitors, Humans, In Situ Nick-End Labeling, Macrophages, Monocytes, Phagocytosis, Reference Values, Regression Analysis, Streptococcus pneumoniae, Time Factors, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416808
URI: http://eprints.soton.ac.uk/id/eprint/416808
ISSN: 0022-1899
PURE UUID: 3fe55c75-bf00-4567-90f0-83e1a0a1c78c
ORCID for R.C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 10 Jan 2018 17:30
Last modified: 16 Mar 2024 04:10

Export record

Altmetrics

Contributors

Author: D.H. Dockrell
Author: M. Lee
Author: D.H. Lynch
Author: R.C. Read ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×