An in vitro model for measuring tryptase release from nasal mucosa in response to allergen challenge
An in vitro model for measuring tryptase release from nasal mucosa in response to allergen challenge
A simple in vitro nasal mucosal culture model has been developed to measure release of the mast cell specific enzyme tryptase in response to allergen challenge. Patients who were undergoing inferior turbinectomy were skin-tested for commonly inhaled allergens. The mucosa from the inferior turbinates was kept viable using Minimal Essential Medium. Tryptase release into the medium was measured using the Pharmacia Riact Assay. There was a significant increase in tryptase release in response to allergen challenge from the mucosa harvested from skin-test positive patients. Mucosa from skin-test negative patients failed to demonstrate an increase in tryptase release. This could prove to be a useful research model for the study of nasal type I hypersensitivity and drugs that affect it.
Allergens, Chymases, Culture Techniques, Humans, Hypersensitivity, Mast Cells, Nasal Mucosa, Rhinitis, Allergic, Perennial, Serine Endopeptidases, Skin Tests, Time Factors, Tryptases, Turbinates, Journal Article
407-409
Mason, J.D.
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Smith, S.J.
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Jones, N.S.
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Majumdar, B.
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Read, R.C.
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October 1994
Mason, J.D.
5d40148f-39f7-4278-9d90-099558386362
Smith, S.J.
d86da701-77fa-4f0f-b016-3017ba94afa5
Jones, N.S.
d2d7b587-4383-454e-aed1-af3cc70d4596
Majumdar, B.
fff48b2c-dac5-40b9-8eee-c7bd6e7aada8
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Mason, J.D., Smith, S.J., Jones, N.S., Majumdar, B. and Read, R.C.
(1994)
An in vitro model for measuring tryptase release from nasal mucosa in response to allergen challenge.
Clinical Otolaryngology and Allied Sciences, 19 (5), .
(doi:10.1111/j.1365-2273.1994.tb01258.x).
Abstract
A simple in vitro nasal mucosal culture model has been developed to measure release of the mast cell specific enzyme tryptase in response to allergen challenge. Patients who were undergoing inferior turbinectomy were skin-tested for commonly inhaled allergens. The mucosa from the inferior turbinates was kept viable using Minimal Essential Medium. Tryptase release into the medium was measured using the Pharmacia Riact Assay. There was a significant increase in tryptase release in response to allergen challenge from the mucosa harvested from skin-test positive patients. Mucosa from skin-test negative patients failed to demonstrate an increase in tryptase release. This could prove to be a useful research model for the study of nasal type I hypersensitivity and drugs that affect it.
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Accepted/In Press date: 18 March 1994
Published date: October 1994
Keywords:
Allergens, Chymases, Culture Techniques, Humans, Hypersensitivity, Mast Cells, Nasal Mucosa, Rhinitis, Allergic, Perennial, Serine Endopeptidases, Skin Tests, Time Factors, Tryptases, Turbinates, Journal Article
Identifiers
Local EPrints ID: 416955
URI: http://eprints.soton.ac.uk/id/eprint/416955
ISSN: 0307-7772
PURE UUID: 33483855-f4e6-46f4-ba05-4415b0eb78c8
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Date deposited: 15 Jan 2018 17:31
Last modified: 16 Mar 2024 04:10
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Contributors
Author:
J.D. Mason
Author:
S.J. Smith
Author:
N.S. Jones
Author:
B. Majumdar
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