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An in vitro model for measuring tryptase release from nasal mucosa in response to allergen challenge

An in vitro model for measuring tryptase release from nasal mucosa in response to allergen challenge
An in vitro model for measuring tryptase release from nasal mucosa in response to allergen challenge

A simple in vitro nasal mucosal culture model has been developed to measure release of the mast cell specific enzyme tryptase in response to allergen challenge. Patients who were undergoing inferior turbinectomy were skin-tested for commonly inhaled allergens. The mucosa from the inferior turbinates was kept viable using Minimal Essential Medium. Tryptase release into the medium was measured using the Pharmacia Riact Assay. There was a significant increase in tryptase release in response to allergen challenge from the mucosa harvested from skin-test positive patients. Mucosa from skin-test negative patients failed to demonstrate an increase in tryptase release. This could prove to be a useful research model for the study of nasal type I hypersensitivity and drugs that affect it.

Allergens, Chymases, Culture Techniques, Humans, Hypersensitivity, Mast Cells, Nasal Mucosa, Rhinitis, Allergic, Perennial, Serine Endopeptidases, Skin Tests, Time Factors, Tryptases, Turbinates, Journal Article
0307-7772
407-409
Mason, J.D.
5d40148f-39f7-4278-9d90-099558386362
Smith, S.J.
d86da701-77fa-4f0f-b016-3017ba94afa5
Jones, N.S.
d2d7b587-4383-454e-aed1-af3cc70d4596
Majumdar, B.
fff48b2c-dac5-40b9-8eee-c7bd6e7aada8
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Mason, J.D.
5d40148f-39f7-4278-9d90-099558386362
Smith, S.J.
d86da701-77fa-4f0f-b016-3017ba94afa5
Jones, N.S.
d2d7b587-4383-454e-aed1-af3cc70d4596
Majumdar, B.
fff48b2c-dac5-40b9-8eee-c7bd6e7aada8
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51

Mason, J.D., Smith, S.J., Jones, N.S., Majumdar, B. and Read, R.C. (1994) An in vitro model for measuring tryptase release from nasal mucosa in response to allergen challenge. Clinical Otolaryngology and Allied Sciences, 19 (5), 407-409. (doi:10.1111/j.1365-2273.1994.tb01258.x).

Record type: Article

Abstract

A simple in vitro nasal mucosal culture model has been developed to measure release of the mast cell specific enzyme tryptase in response to allergen challenge. Patients who were undergoing inferior turbinectomy were skin-tested for commonly inhaled allergens. The mucosa from the inferior turbinates was kept viable using Minimal Essential Medium. Tryptase release into the medium was measured using the Pharmacia Riact Assay. There was a significant increase in tryptase release in response to allergen challenge from the mucosa harvested from skin-test positive patients. Mucosa from skin-test negative patients failed to demonstrate an increase in tryptase release. This could prove to be a useful research model for the study of nasal type I hypersensitivity and drugs that affect it.

Full text not available from this repository.

More information

Accepted/In Press date: 18 March 1994
Published date: October 1994
Keywords: Allergens, Chymases, Culture Techniques, Humans, Hypersensitivity, Mast Cells, Nasal Mucosa, Rhinitis, Allergic, Perennial, Serine Endopeptidases, Skin Tests, Time Factors, Tryptases, Turbinates, Journal Article

Identifiers

Local EPrints ID: 416955
URI: https://eprints.soton.ac.uk/id/eprint/416955
ISSN: 0307-7772
PURE UUID: 33483855-f4e6-46f4-ba05-4415b0eb78c8
ORCID for R.C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 15 Jan 2018 17:31
Last modified: 14 Mar 2019 01:36

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