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Induction of beta2 integrin-dependent neutrophil adhesion to human alveolar epithelial cells by type 1 Streptococcus pneumoniae and derived soluble factors

Induction of beta2 integrin-dependent neutrophil adhesion to human alveolar epithelial cells by type 1 Streptococcus pneumoniae and derived soluble factors
Induction of beta2 integrin-dependent neutrophil adhesion to human alveolar epithelial cells by type 1 Streptococcus pneumoniae and derived soluble factors

Pneumonia caused by Streptococcus pneumoniae is characterized by neutrophil infiltration and variable epithelial injury. Neutrophil adhesion to alveolar epithelial pneumocytes (A549) was measured and demonstrated to be dose-dependent following preincubation of these (A549) pneumocytes with type 1 S. pneumoniae. Adhesion peaked at a bacteria-to-epithelial cell ratio of 5:1 after a 4-h incubation but was absent after 2 h and without FMLP. Filtered conditioned media (CM) from pneumococci cultured with (CM+) or without (CM-) epithelial cells were tested. CM+ induced significant adhesion in the absence of FMLP (P < .001); CM- had no effect. In the presence of FMLP, adhesion induced by both media was significantly greater than by FMLP alone (P < .001) and was significantly blocked (P < .01) by antibodies to CD11b and CD18. CM+ upregulated epithelial intercellular adhesion molecule 1 but CM- did not. These data provide new information concerning the interactions of S. pneumoniae, alveolar epithelial cells, and neutrophils.

Alkaline Phosphatase, Antibodies, Blocking, Antibodies, Monoclonal, CD11 Antigens, CD18 Antigens, Cell Adhesion, Cells, Cultured, Culture Media, Conditioned, Epithelial Cells, Epithelium, Flow Cytometry, Fluorescent Antibody Technique, Indirect, Humans, Intercellular Adhesion Molecule-1, N-Formylmethionine Leucyl-Phenylalanine, Neutrophils, Pneumonia, Pneumococcal, Streptococcus pneumoniae, Up-Regulation, Journal Article, Research Support, Non-U.S. Gov't
0022-1899
977-985
Smith, J.D.
36614739-504f-48d7-9455-afef5cc4663d
Cortes, N.J.
cafdf2ff-4caf-42ad-a490-6c1fa754fbb2
Evans, G.S.
597245f5-589d-4a02-bb88-67f10b37cae0
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Finn, A.
51e100d7-bd49-442f-b50d-240841fa25f0
Smith, J.D.
36614739-504f-48d7-9455-afef5cc4663d
Cortes, N.J.
cafdf2ff-4caf-42ad-a490-6c1fa754fbb2
Evans, G.S.
597245f5-589d-4a02-bb88-67f10b37cae0
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Finn, A.
51e100d7-bd49-442f-b50d-240841fa25f0

Smith, J.D., Cortes, N.J., Evans, G.S., Read, R.C. and Finn, A. (1998) Induction of beta2 integrin-dependent neutrophil adhesion to human alveolar epithelial cells by type 1 Streptococcus pneumoniae and derived soluble factors. The Journal of Infectious Diseases, 177 (4), 977-985. (doi:10.1086/515235).

Record type: Article

Abstract

Pneumonia caused by Streptococcus pneumoniae is characterized by neutrophil infiltration and variable epithelial injury. Neutrophil adhesion to alveolar epithelial pneumocytes (A549) was measured and demonstrated to be dose-dependent following preincubation of these (A549) pneumocytes with type 1 S. pneumoniae. Adhesion peaked at a bacteria-to-epithelial cell ratio of 5:1 after a 4-h incubation but was absent after 2 h and without FMLP. Filtered conditioned media (CM) from pneumococci cultured with (CM+) or without (CM-) epithelial cells were tested. CM+ induced significant adhesion in the absence of FMLP (P < .001); CM- had no effect. In the presence of FMLP, adhesion induced by both media was significantly greater than by FMLP alone (P < .001) and was significantly blocked (P < .01) by antibodies to CD11b and CD18. CM+ upregulated epithelial intercellular adhesion molecule 1 but CM- did not. These data provide new information concerning the interactions of S. pneumoniae, alveolar epithelial cells, and neutrophils.

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More information

Published date: April 1998
Keywords: Alkaline Phosphatase, Antibodies, Blocking, Antibodies, Monoclonal, CD11 Antigens, CD18 Antigens, Cell Adhesion, Cells, Cultured, Culture Media, Conditioned, Epithelial Cells, Epithelium, Flow Cytometry, Fluorescent Antibody Technique, Indirect, Humans, Intercellular Adhesion Molecule-1, N-Formylmethionine Leucyl-Phenylalanine, Neutrophils, Pneumonia, Pneumococcal, Streptococcus pneumoniae, Up-Regulation, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416967
URI: http://eprints.soton.ac.uk/id/eprint/416967
ISSN: 0022-1899
PURE UUID: 32154efb-2e51-46b4-8b09-018b4553f0e1
ORCID for R.C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 15 Jan 2018 17:31
Last modified: 10 Nov 2021 03:30

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Contributors

Author: J.D. Smith
Author: N.J. Cortes
Author: G.S. Evans
Author: R.C. Read ORCID iD
Author: A. Finn

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