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Coinfection with influenza B virus does not affect association of Neisseria meningitidis with human nasopharyngeal mucosa in organ culture

Coinfection with influenza B virus does not affect association of Neisseria meningitidis with human nasopharyngeal mucosa in organ culture
Coinfection with influenza B virus does not affect association of Neisseria meningitidis with human nasopharyngeal mucosa in organ culture

There is an epidemiological association between influenza virus infection and meningococcal disease. Proposed mechanisms are the destruction of the normal epithelial barrier function of the upper respiratory tract by influenza virus or the expression of human or viral surface-exposed proteins that enhance bacterial adherence and/or invasion. To test these hypotheses, human nasopharyngeal mucosa specimens from a total of 19 individual donors were successfully infected with influenza B virus and then inoculated with serogroup B Neisseria meningitidis. Subsequent bacterial association with the epithelial surface was measured in three separate series of experiments by using transmission electron microscopy (n = 6), scanning electron microscopy (n = 6), and counting of viable bacteria within homogenates of explants (n = 7). Penetration of the mucosa was estimated by measuring the count of viable bacteria recovered from explants after exposure to sodium taurocholate. Bacterial association with the surface of explants was time dependent over 24 h of superinfection. Influenza virus did not positively or negatively influence bacterial attachment to or penetration of explant mucosa compared to those of uninfected controls, even when the period of preincubation with virus was extended to 7 days. When proteins were purified from mucosal epithelium and immobilized on nitrocellulose membranes, N. meningitidis attached predominantly to bands corresponding to proteins of 210 and 130 kDa. In the presence of influenza virus infection, these proteins were gradually lost over the course of 72 h. In conclusion, influenza B virus did not increase association of serogroup B N. meningitidis with human nasopharyngeal mucosa.

Antigens, Viral, Epithelium, Humans, Influenza B virus, Nasal Mucosa, Nasopharynx, Neisseria meningitidis, Organ Culture Techniques, Journal Article, Research Support, Non-U.S. Gov't
0019-9567
3082-3086
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Goodwin, L.
45c48038-ca4d-451a-9fbe-b4d6ea95048b
Parsons, M.A.
0cf09dbb-a13f-4bbe-8b4a-a1d58043f9ff
Silcocks, P.
c02fb003-fe21-4172-abc0-27ebafe6df3e
Kaczmarski, E.B.
b64d93b8-aaee-44f6-a7a3-715f8971d3b3
Parker, A.
2060d83a-15d6-444c-82f3-a0e4e549dcaa
Baldwin, T.J.
342bc2f8-3b5e-4f9b-9cd7-96920883ee21
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Goodwin, L.
45c48038-ca4d-451a-9fbe-b4d6ea95048b
Parsons, M.A.
0cf09dbb-a13f-4bbe-8b4a-a1d58043f9ff
Silcocks, P.
c02fb003-fe21-4172-abc0-27ebafe6df3e
Kaczmarski, E.B.
b64d93b8-aaee-44f6-a7a3-715f8971d3b3
Parker, A.
2060d83a-15d6-444c-82f3-a0e4e549dcaa
Baldwin, T.J.
342bc2f8-3b5e-4f9b-9cd7-96920883ee21

Read, R.C., Goodwin, L., Parsons, M.A., Silcocks, P., Kaczmarski, E.B., Parker, A. and Baldwin, T.J. (1999) Coinfection with influenza B virus does not affect association of Neisseria meningitidis with human nasopharyngeal mucosa in organ culture. Infection and Immunity, 67 (6), 3082-3086.

Record type: Article

Abstract

There is an epidemiological association between influenza virus infection and meningococcal disease. Proposed mechanisms are the destruction of the normal epithelial barrier function of the upper respiratory tract by influenza virus or the expression of human or viral surface-exposed proteins that enhance bacterial adherence and/or invasion. To test these hypotheses, human nasopharyngeal mucosa specimens from a total of 19 individual donors were successfully infected with influenza B virus and then inoculated with serogroup B Neisseria meningitidis. Subsequent bacterial association with the epithelial surface was measured in three separate series of experiments by using transmission electron microscopy (n = 6), scanning electron microscopy (n = 6), and counting of viable bacteria within homogenates of explants (n = 7). Penetration of the mucosa was estimated by measuring the count of viable bacteria recovered from explants after exposure to sodium taurocholate. Bacterial association with the surface of explants was time dependent over 24 h of superinfection. Influenza virus did not positively or negatively influence bacterial attachment to or penetration of explant mucosa compared to those of uninfected controls, even when the period of preincubation with virus was extended to 7 days. When proteins were purified from mucosal epithelium and immobilized on nitrocellulose membranes, N. meningitidis attached predominantly to bands corresponding to proteins of 210 and 130 kDa. In the presence of influenza virus infection, these proteins were gradually lost over the course of 72 h. In conclusion, influenza B virus did not increase association of serogroup B N. meningitidis with human nasopharyngeal mucosa.

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More information

Published date: June 1999
Keywords: Antigens, Viral, Epithelium, Humans, Influenza B virus, Nasal Mucosa, Nasopharynx, Neisseria meningitidis, Organ Culture Techniques, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416969
URI: http://eprints.soton.ac.uk/id/eprint/416969
ISSN: 0019-9567
PURE UUID: 892dda34-f083-436c-8a35-52d5046d5243
ORCID for R.C. Read: ORCID iD orcid.org/0000-0002-4297-6728

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Date deposited: 15 Jan 2018 17:31
Last modified: 16 Mar 2024 04:10

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Contributors

Author: R.C. Read ORCID iD
Author: L. Goodwin
Author: M.A. Parsons
Author: P. Silcocks
Author: E.B. Kaczmarski
Author: A. Parker
Author: T.J. Baldwin

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