The effects of activated eosinophils and neutrophils on guinea pig airway epithelium in vitro
The effects of activated eosinophils and neutrophils on guinea pig airway epithelium in vitro
Epithelial shedding is a characteristic feature of asthmatic airways and has been attributed to eosinophil products. We have examined the interaction of purified intraperitoneal guinea pig eosinophils with or without platelet-activating factor (PAF, 10(-7) M) or lyso-PAF (10(-7) M) with guinea pig tracheal epithelium in vitro. At 0, 4, 14, and 24 h, the percentage of ciliation of the tracheal circumference (CTC) was measured by light microscopy and the ciliary beat frequency (CBF) by photometry. PAF-activated eosinophils (50 x 10(6) cells/ml) disrupted the epithelium, mean CBF and CTC being reduced by 77.8 +/- 5.8% (mean +/- SEM; P less than 0.001 versus control) and 94.2 +/- 1.4% (P less than 0.001) over 24 h, respectively. PAF (10(-7) M) alone had no significant effect. Lyso-PAF with eosinophils (50 x 10(6) cells/ml) also reduced mean CBF and CTC but to a lesser extent. Eosinophils alone also led to a reduction of 36.2 +/- 11.4% in mean CBF and 53.0 +/- 15.5% in CTC, but these changes were not significant. The PAF antagonist, WEB 2086 (10(-6) M), significantly inhibited the mean CBF and CTC reduction due to PAF-activated eosinophils by 61.5 +/- 17.2% (P less than 0.01) and 20.8 +/- 6.5% (P less than 0.05), respectively. In addition, catalase (1,125 U/ml) partially inhibited the mean CBF and CTC reduction induced by PAF-activated eosinophils. Intraperitoneal neutrophils (PMN) (50 x 10(6) cells/ml) also disrupted epithelium but to a lesser extent (24-h reduction: 34.2 +/- 12.7% for mean CBF and 60.2 +/- 13.2% for CTC, respectively). Stimulation with PAF (10(-7) M) had no further effect. Marked exfoliation of the epithelial layer was observed after 14 h of incubation with activated eosinophils. We concluded the PAF-activated eosinophils are capable of grossly disrupting ciliated epithelium and may contribute to epithelial damage observed in asthma.
Animals, Azepines, Catalase, Cell Survival, Cilia, Eosinophils, Epithelium, Guinea Pigs, Male, Neutrophils, Peritoneal Cavity, Platelet Activating Factor, Trachea, Triazines, Triazoles, Journal Article, Research Support, Non-U.S. Gov't
341-353
Yukawa, T.
185fbfa7-b531-4c3f-8ae2-ff0ab73ded59
Read, R.C.
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Kroegel, C.
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Rutman, A.
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Chung, K.F.
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Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Cole, P.J.
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Barnes, P.J.
0cd31428-a5db-4725-a1d5-0a89ddfd8b86
April 1990
Yukawa, T.
185fbfa7-b531-4c3f-8ae2-ff0ab73ded59
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Kroegel, C.
29afe82c-4e62-4063-9568-f1469d59c3d7
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Chung, K.F.
5690488b-509e-4632-ace7-2221fc5b453e
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3
Barnes, P.J.
0cd31428-a5db-4725-a1d5-0a89ddfd8b86
Yukawa, T., Read, R.C., Kroegel, C., Rutman, A., Chung, K.F., Wilson, R., Cole, P.J. and Barnes, P.J.
(1990)
The effects of activated eosinophils and neutrophils on guinea pig airway epithelium in vitro.
American Journal of Respiratory Cell and Molecular Biology, 2 (4), .
(doi:10.1165/ajrcmb/2.4.341).
Abstract
Epithelial shedding is a characteristic feature of asthmatic airways and has been attributed to eosinophil products. We have examined the interaction of purified intraperitoneal guinea pig eosinophils with or without platelet-activating factor (PAF, 10(-7) M) or lyso-PAF (10(-7) M) with guinea pig tracheal epithelium in vitro. At 0, 4, 14, and 24 h, the percentage of ciliation of the tracheal circumference (CTC) was measured by light microscopy and the ciliary beat frequency (CBF) by photometry. PAF-activated eosinophils (50 x 10(6) cells/ml) disrupted the epithelium, mean CBF and CTC being reduced by 77.8 +/- 5.8% (mean +/- SEM; P less than 0.001 versus control) and 94.2 +/- 1.4% (P less than 0.001) over 24 h, respectively. PAF (10(-7) M) alone had no significant effect. Lyso-PAF with eosinophils (50 x 10(6) cells/ml) also reduced mean CBF and CTC but to a lesser extent. Eosinophils alone also led to a reduction of 36.2 +/- 11.4% in mean CBF and 53.0 +/- 15.5% in CTC, but these changes were not significant. The PAF antagonist, WEB 2086 (10(-6) M), significantly inhibited the mean CBF and CTC reduction due to PAF-activated eosinophils by 61.5 +/- 17.2% (P less than 0.01) and 20.8 +/- 6.5% (P less than 0.05), respectively. In addition, catalase (1,125 U/ml) partially inhibited the mean CBF and CTC reduction induced by PAF-activated eosinophils. Intraperitoneal neutrophils (PMN) (50 x 10(6) cells/ml) also disrupted epithelium but to a lesser extent (24-h reduction: 34.2 +/- 12.7% for mean CBF and 60.2 +/- 13.2% for CTC, respectively). Stimulation with PAF (10(-7) M) had no further effect. Marked exfoliation of the epithelial layer was observed after 14 h of incubation with activated eosinophils. We concluded the PAF-activated eosinophils are capable of grossly disrupting ciliated epithelium and may contribute to epithelial damage observed in asthma.
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Published date: April 1990
Keywords:
Animals, Azepines, Catalase, Cell Survival, Cilia, Eosinophils, Epithelium, Guinea Pigs, Male, Neutrophils, Peritoneal Cavity, Platelet Activating Factor, Trachea, Triazines, Triazoles, Journal Article, Research Support, Non-U.S. Gov't
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Local EPrints ID: 416998
URI: http://eprints.soton.ac.uk/id/eprint/416998
ISSN: 1044-1549
PURE UUID: b605925e-4696-4543-a87c-36c7c58866b3
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Date deposited: 16 Jan 2018 17:30
Last modified: 16 Mar 2024 04:10
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Author:
T. Yukawa
Author:
C. Kroegel
Author:
A. Rutman
Author:
K.F. Chung
Author:
R. Wilson
Author:
P.J. Cole
Author:
P.J. Barnes
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