The University of Southampton
University of Southampton Institutional Repository

The effects of activated eosinophils and neutrophils on guinea pig airway epithelium in vitro

The effects of activated eosinophils and neutrophils on guinea pig airway epithelium in vitro
The effects of activated eosinophils and neutrophils on guinea pig airway epithelium in vitro

Epithelial shedding is a characteristic feature of asthmatic airways and has been attributed to eosinophil products. We have examined the interaction of purified intraperitoneal guinea pig eosinophils with or without platelet-activating factor (PAF, 10(-7) M) or lyso-PAF (10(-7) M) with guinea pig tracheal epithelium in vitro. At 0, 4, 14, and 24 h, the percentage of ciliation of the tracheal circumference (CTC) was measured by light microscopy and the ciliary beat frequency (CBF) by photometry. PAF-activated eosinophils (50 x 10(6) cells/ml) disrupted the epithelium, mean CBF and CTC being reduced by 77.8 +/- 5.8% (mean +/- SEM; P less than 0.001 versus control) and 94.2 +/- 1.4% (P less than 0.001) over 24 h, respectively. PAF (10(-7) M) alone had no significant effect. Lyso-PAF with eosinophils (50 x 10(6) cells/ml) also reduced mean CBF and CTC but to a lesser extent. Eosinophils alone also led to a reduction of 36.2 +/- 11.4% in mean CBF and 53.0 +/- 15.5% in CTC, but these changes were not significant. The PAF antagonist, WEB 2086 (10(-6) M), significantly inhibited the mean CBF and CTC reduction due to PAF-activated eosinophils by 61.5 +/- 17.2% (P less than 0.01) and 20.8 +/- 6.5% (P less than 0.05), respectively. In addition, catalase (1,125 U/ml) partially inhibited the mean CBF and CTC reduction induced by PAF-activated eosinophils. Intraperitoneal neutrophils (PMN) (50 x 10(6) cells/ml) also disrupted epithelium but to a lesser extent (24-h reduction: 34.2 +/- 12.7% for mean CBF and 60.2 +/- 13.2% for CTC, respectively). Stimulation with PAF (10(-7) M) had no further effect. Marked exfoliation of the epithelial layer was observed after 14 h of incubation with activated eosinophils. We concluded the PAF-activated eosinophils are capable of grossly disrupting ciliated epithelium and may contribute to epithelial damage observed in asthma.

Animals, Azepines, Catalase, Cell Survival, Cilia, Eosinophils, Epithelium, Guinea Pigs, Male, Neutrophils, Peritoneal Cavity, Platelet Activating Factor, Trachea, Triazines, Triazoles, Journal Article, Research Support, Non-U.S. Gov't
1044-1549
341-353
Yukawa, T.
185fbfa7-b531-4c3f-8ae2-ff0ab73ded59
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Kroegel, C.
29afe82c-4e62-4063-9568-f1469d59c3d7
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Chung, K.F.
5690488b-509e-4632-ace7-2221fc5b453e
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3
Barnes, P.J.
0cd31428-a5db-4725-a1d5-0a89ddfd8b86
Yukawa, T.
185fbfa7-b531-4c3f-8ae2-ff0ab73ded59
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Kroegel, C.
29afe82c-4e62-4063-9568-f1469d59c3d7
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Chung, K.F.
5690488b-509e-4632-ace7-2221fc5b453e
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3
Barnes, P.J.
0cd31428-a5db-4725-a1d5-0a89ddfd8b86

Yukawa, T., Read, R.C., Kroegel, C., Rutman, A., Chung, K.F., Wilson, R., Cole, P.J. and Barnes, P.J. (1990) The effects of activated eosinophils and neutrophils on guinea pig airway epithelium in vitro. American Journal of Respiratory Cell and Molecular Biology, 2 (4), 341-353. (doi:10.1165/ajrcmb/2.4.341).

Record type: Article

Abstract

Epithelial shedding is a characteristic feature of asthmatic airways and has been attributed to eosinophil products. We have examined the interaction of purified intraperitoneal guinea pig eosinophils with or without platelet-activating factor (PAF, 10(-7) M) or lyso-PAF (10(-7) M) with guinea pig tracheal epithelium in vitro. At 0, 4, 14, and 24 h, the percentage of ciliation of the tracheal circumference (CTC) was measured by light microscopy and the ciliary beat frequency (CBF) by photometry. PAF-activated eosinophils (50 x 10(6) cells/ml) disrupted the epithelium, mean CBF and CTC being reduced by 77.8 +/- 5.8% (mean +/- SEM; P less than 0.001 versus control) and 94.2 +/- 1.4% (P less than 0.001) over 24 h, respectively. PAF (10(-7) M) alone had no significant effect. Lyso-PAF with eosinophils (50 x 10(6) cells/ml) also reduced mean CBF and CTC but to a lesser extent. Eosinophils alone also led to a reduction of 36.2 +/- 11.4% in mean CBF and 53.0 +/- 15.5% in CTC, but these changes were not significant. The PAF antagonist, WEB 2086 (10(-6) M), significantly inhibited the mean CBF and CTC reduction due to PAF-activated eosinophils by 61.5 +/- 17.2% (P less than 0.01) and 20.8 +/- 6.5% (P less than 0.05), respectively. In addition, catalase (1,125 U/ml) partially inhibited the mean CBF and CTC reduction induced by PAF-activated eosinophils. Intraperitoneal neutrophils (PMN) (50 x 10(6) cells/ml) also disrupted epithelium but to a lesser extent (24-h reduction: 34.2 +/- 12.7% for mean CBF and 60.2 +/- 13.2% for CTC, respectively). Stimulation with PAF (10(-7) M) had no further effect. Marked exfoliation of the epithelial layer was observed after 14 h of incubation with activated eosinophils. We concluded the PAF-activated eosinophils are capable of grossly disrupting ciliated epithelium and may contribute to epithelial damage observed in asthma.

Full text not available from this repository.

More information

Published date: April 1990
Keywords: Animals, Azepines, Catalase, Cell Survival, Cilia, Eosinophils, Epithelium, Guinea Pigs, Male, Neutrophils, Peritoneal Cavity, Platelet Activating Factor, Trachea, Triazines, Triazoles, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 416998
URI: https://eprints.soton.ac.uk/id/eprint/416998
ISSN: 1044-1549
PURE UUID: b605925e-4696-4543-a87c-36c7c58866b3
ORCID for R.C. Read: ORCID iD orcid.org/0000-0002-4297-6728

Catalogue record

Date deposited: 16 Jan 2018 17:30
Last modified: 14 Mar 2019 01:36

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×