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Interaction of nontypable Haemophilus influenzae with human respiratory mucosa in vitro

Interaction of nontypable Haemophilus influenzae with human respiratory mucosa in vitro
Interaction of nontypable Haemophilus influenzae with human respiratory mucosa in vitro

One laboratory strain (SH9) (n = 12) and five clinical isolates of unencapsulated Haemophilus influenzae replicated from 10(4) to 10(8) cfu/ml over 24 h in an organ culture of human respiratory mucosa in which only the intact mucosal surface is exposed. By transmission electron microscopy (TEM), bacteria were not seen in association with normal respiratory epithelium, even after incubation for 24 h. Histology and TEM morphometry demonstrated patchy and occasionally confluent damage to epithelia at this time, with bacteria associated only with cells that were structurally damaged. Scanning electron microscopy revealed an increased quantity of mucus in infected preparations; H. influenzae were associated with mucus by 14 h of incubation and with damaged epithelial cells by 24 h. Fimbriation of H. influenzae increased buccal cell adherence but did not facilitate association with normal respiratory epithelium and failed to increase epithelial damage or association with damaged cells. Epithelial damage may be prerequisite for association of H. influenzae with respiratory epithelium in vitro.

Bacterial Adhesion, Fimbriae, Bacterial, Haemophilus influenzae, Humans, Mucociliary Clearance, Mucous Membrane, Organ Culture Techniques, Respiratory System, Time Factors, Journal Article, Research Support, Non-U.S. Gov't
0022-1899
549-558
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Lund, V.
19b0121c-1518-4cd8-8c2b-be8557320b0f
Todd, H.C.
02707687-9f39-4a4c-ad8b-3c5476d8a71e
Brain, A.P.
6f66f21b-f604-463f-bc7c-63e9d5811112
Jeffery, P.K.
c06724b0-d87f-45e7-86f8-4dc1a12f8877
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3
Read, R.C.
b5caca7b-0063-438a-b703-7ecbb6fc2b51
Wilson, R.
94eba368-b33f-4071-beb6-dac98ef23651
Rutman, A.
b06264ba-bff8-4e7b-b3af-868cfe6369e6
Lund, V.
19b0121c-1518-4cd8-8c2b-be8557320b0f
Todd, H.C.
02707687-9f39-4a4c-ad8b-3c5476d8a71e
Brain, A.P.
6f66f21b-f604-463f-bc7c-63e9d5811112
Jeffery, P.K.
c06724b0-d87f-45e7-86f8-4dc1a12f8877
Cole, P.J.
8d93f747-e6ed-4557-89ea-e12e0925bbf3

Read, R.C., Wilson, R., Rutman, A., Lund, V., Todd, H.C., Brain, A.P., Jeffery, P.K. and Cole, P.J. (1991) Interaction of nontypable Haemophilus influenzae with human respiratory mucosa in vitro. The Journal of Infectious Diseases, 163 (3), 549-558. (doi:10.1093/infdis/163.3.549).

Record type: Article

Abstract

One laboratory strain (SH9) (n = 12) and five clinical isolates of unencapsulated Haemophilus influenzae replicated from 10(4) to 10(8) cfu/ml over 24 h in an organ culture of human respiratory mucosa in which only the intact mucosal surface is exposed. By transmission electron microscopy (TEM), bacteria were not seen in association with normal respiratory epithelium, even after incubation for 24 h. Histology and TEM morphometry demonstrated patchy and occasionally confluent damage to epithelia at this time, with bacteria associated only with cells that were structurally damaged. Scanning electron microscopy revealed an increased quantity of mucus in infected preparations; H. influenzae were associated with mucus by 14 h of incubation and with damaged epithelial cells by 24 h. Fimbriation of H. influenzae increased buccal cell adherence but did not facilitate association with normal respiratory epithelium and failed to increase epithelial damage or association with damaged cells. Epithelial damage may be prerequisite for association of H. influenzae with respiratory epithelium in vitro.

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More information

Published date: March 1991
Keywords: Bacterial Adhesion, Fimbriae, Bacterial, Haemophilus influenzae, Humans, Mucociliary Clearance, Mucous Membrane, Organ Culture Techniques, Respiratory System, Time Factors, Journal Article, Research Support, Non-U.S. Gov't

Identifiers

Local EPrints ID: 417001
URI: https://eprints.soton.ac.uk/id/eprint/417001
ISSN: 0022-1899
PURE UUID: 1c093022-c4dc-48df-967d-aa90a3fdd786
ORCID for R.C. Read: ORCID iD orcid.org/0000-0002-4297-6728

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Date deposited: 16 Jan 2018 17:30
Last modified: 14 Mar 2019 01:36

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Contributors

Author: R.C. Read ORCID iD
Author: R. Wilson
Author: A. Rutman
Author: V. Lund
Author: H.C. Todd
Author: A.P. Brain
Author: P.K. Jeffery
Author: P.J. Cole

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